QTL mapping for salt tolerance and domestication-related traits in Vigna marina subsp. oblonga, a halophytic species
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QTL mapping in F 2 population [ V. luteola × V. marina subsp. oblonga ] revealed that the salt tolerance in V. marina subsp. oblonga is controlled by a single major QTL.
The habitats of beach cowpea (Vigna marina) are sandy beaches in tropical and subtropical regions. As a species that grows closest to the sea, it has potential to be a gene source for breeding salt-tolerant crops. We reported here for the first time, quantitative trait loci (QTLs) mapping for salt tolerance in V. marina. A genetic linkage map was constructed from an F2 population of 120 plants derived from an interspecific cross between V. luteola and V. marina subsp. oblonga. The map comprised 150 SSR markers. The markers were clustered into 11 linkage groups spanning 777.6 cM in length with a mean distance between the adjacent markers of 5.59 cM. The F2:3 population was evaluated for salt tolerance under hydroponic conditions at the seedling and developmental stages. Segregation analysis indicated that salt tolerance in V. marina is controlled by a few genes. Multiple interval mapping consistently identified one major QTL which can explain about 50 % of phenotypic variance. The flanking markers may facilitate transfer of the salt tolerance allele from V. marina subsp. oblonga into related Vigna crops. The QTL for domestication-related traits from V. marina are also discussed.
KeywordsQuantitative Trait Locus Salt Tolerance Common Bean Quantitative Trait Locus Analysis Quantitative Trait Locus Mapping
This study was supported by JST, PRESTO to K. Naito, and by the Royal Golden Jubilee (RGJ) Ph.D. Program jointly funded by the Thailand Research Fund (TRF) and Kasetsart University to P. Srinives and S. Chankaew.
Conflict of interest
The authors declare that they have no conflict of interest.
All the experiments carried out in this study comply with the current laws of both Thailand and Japan.
- FAO (2005) Global network on integrated soil management for sustainable use of salt-affected soils. FAO land and plant nutrition management service, RomeGoogle Scholar
- Kaga A, Vaughan DA, Tomooka N (2005) Molecular markers in Vigna improvement: understanding and using gene pools. In: Lorz H, Wanzel G (eds) Biotechnology in agriculture and forestry, vol 55., Molecular marker systemsBerlin, Springer, pp 171–187Google Scholar
- Lawn RJ, Cottrell A (1988) Wild mungbean and its relatives in Australia. Biologist 35:267–273Google Scholar
- Lee SH (2012) Toward completion of genome sequence of mungbean. In: Abstract book, VI International Conference on Legume Genetics and Genomics, 2–7 October 2012, Hyderabad, IndiaGoogle Scholar
- Leonforte A, Sudheesh S, Cogan NOI, Salisbury PA, Nicolas ME, Materne M, Forster JW, Kaur S (2013) SNP marker discovery, linkage map construction and identification of QTLs for enhanced salinity tolerance in field pea (Pisum sativum L.). BMC Plant Biol 13:161. doi: 10.1186/1471-2229-13-161 PubMedCrossRefGoogle Scholar
- Lewis G, Schrire B, Mackinder B, Lock M (2005) Legumes of the world. Kew, Royal Botanic GardensGoogle Scholar
- Lobato AKS, Santos Filho BG, Costa RCL, Goncalva-Vidigal MC, Moraes EC, Oliveira Neto CF, Rodriges VLF, Cruz FJR, Ferreira AS, Pita JD, Barreto AGT (2009) Morphological, physiological and biochemical responses during germination of the cowpea (Vigna unguiculata cv. Pitiuba) seeds under salt stress. World J Agric Sci 5:590–596Google Scholar
- Maréchal R, Mascherpa JM, Stainer F (1978) Extude taxonomique d’un groupe d’espèces des genres Phaseolus et Vigna (V. unguiculata) sur la base de données morphologiques et polliniques, traitées pour l’analyse informatique. Boissiera 28:160–272Google Scholar
- Mehra KL, Ibrahim AH (1989) Genetic resources in the Maldives. FAO/IBPGR Plant Genet Resour Newsl 75(76):42–43Google Scholar
- Samineni S (2010) Physiology, genetics and QTL mapping of salt tolerance in chickpea (Cicer arietinum L.). Ph.D. Thesis, The University of Western AustraliaGoogle Scholar
- Tangphatsornruang S, Somta P, Uthaipaisanwong P, Chanprasert J, Sangsrakru D, Seehalak W, Sommanas W, Tragoonrung S, Srinives P (2009) Characterization of microsatellites and gene contents from genome shotgun sequences of mungbean (Vigna radiata (L.) Wilczek). BMC Plant Biol 9:137PubMedCentralPubMedCrossRefGoogle Scholar
- Van Ooijen JW (2006) JoinMap version 4.0, software for the calculation of genetic linkage maps. Kyazma B.V, WageningenGoogle Scholar
- Verdcourt B (1971) Phaseoleae. In: Milne-Redhead E, Polhill RM (eds) Flora of tropical East Africa, Leguminosae (part 4), Papilionideae (2). Crown Agents for Overseas Governments, London, pp 625–627Google Scholar
- Wang S, Basten CJ, Zeng ZB (2007) Windows QTL Cartographer 2.5. North Carolina State University, RaleighGoogle Scholar
- Wilson C, Liu X, Lesch SM, Suarez DL (2009) Growth response of major U.S. cowpea cultivars. I. Biomass accumulation and salt tolerance. Hortic Sci 41:225–230Google Scholar
- Win KT, Oo AZ, Hirasawa T, Ookawa T, Yutaka H (2011) Genetic analysis of Myanmar Vigna species in response to salt stress at the seedling stage. Afr J Biotechnol 10:1615–1624Google Scholar
- Zhang LP, Lin GY, Foolad MR (2003) QTL comparison of salt tolerance during seed germination and vegetative growth in a Lycopersicon esculentum × L. pimpinellifolium RIL population. Acta Hort 618:59–67Google Scholar