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Mapping and identification of a Cicer arietinum NSP2 gene involved in nodulation pathway

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For the first time the putative NSP2 gene in chickpea has been identified using pairs of NILs differing for the Rn1 / rn1 nodulation gene that was located in LG5 of chickpea genetic map.

Abstract

An intraspecific cross between the mutant non-nodulating genotype PM233, carrying the recessive gene rn1, and the wild-type CA2139 was used to develop two pairs of near-isogenic lines (NILs) for nodulation in chickpea. These pairs of NILs were characterized using sequence tagged microsatellite site (STMS) markers distributed across different linkage groups (LGs) of the chickpea genetic map leading to the detection of polymorphic markers located in LG5. Using this information, together with the genome annotation in Medicago truncatula, a candidate gene (NSP2) known to be involved in nodulation pathway was selected for mapping in chickpea. The full length sequence obtained in chickpea wild-type (CaNSP2) was 1,503 bp. Linkage analysis in an F3 population of 118 plants derived from the cross between the pair of NILS NIL7-2A (nod) × NIL7-2B (non-nod) revealed a co-localization between CaNSP2 and Rn1 gene. These data implicate the CaNSP2 gene as a candidate for identity to Rn1, and suggest that it could act in the nodulation signaling transduction pathway similarly to that in other legumes species.

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Acknowledgments

This work has been supported by the project INIA contract RTA2010-00059, co-financed by EU funds (FEDER). We are also indebted to Dr. F. Temprano (IFAPA, Spain) for his support with Rhizobium inoculations. Ali L acknowledges PhD fellowship from Syrian Ministry of High Education and ICRISAT for supporting 6 months stay at the Center of Excellence in Genomics, ICRISAT, Patancheru, India (http://www.icrisat.org/ceg).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Dpto Genética, Univ Córdoba, Campus de excelencia internacional CeiA3, Campus de Rabanales, 14071, Córdoba, Spain

    L. Ali, T. Millan & J. Gil

  2. Institute for Sustainable Agriculture, CSIC, 4084, 14080, Córdoba, Spain

    E. Madrid

  3. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, Andhra Pradesh, India

    R. K. Varshney & S. Azam

  4. Área de Mejora y Biotecnología, IFAPA Centro “Alameda del Obispo”, Apdo 3092, 14080, Córdoba, Spain

    J. Rubio

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  1. L. Ali
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Correspondence to L. Ali.

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Communicated by A. E. Melchinger.

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Ali, L., Madrid, E., Varshney, R.K. et al. Mapping and identification of a Cicer arietinum NSP2 gene involved in nodulation pathway. Theor Appl Genet 127, 481–488 (2014). https://doi.org/10.1007/s00122-013-2233-3

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  • DOI: https://doi.org/10.1007/s00122-013-2233-3

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