Mapping and identification of a Cicer arietinum NSP2 gene involved in nodulation pathway
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For the first time the putative NSP2 gene in chickpea has been identified using pairs of NILs differing for the Rn1 / rn1 nodulation gene that was located in LG5 of chickpea genetic map.
An intraspecific cross between the mutant non-nodulating genotype PM233, carrying the recessive gene rn1, and the wild-type CA2139 was used to develop two pairs of near-isogenic lines (NILs) for nodulation in chickpea. These pairs of NILs were characterized using sequence tagged microsatellite site (STMS) markers distributed across different linkage groups (LGs) of the chickpea genetic map leading to the detection of polymorphic markers located in LG5. Using this information, together with the genome annotation in Medicago truncatula, a candidate gene (NSP2) known to be involved in nodulation pathway was selected for mapping in chickpea. The full length sequence obtained in chickpea wild-type (CaNSP2) was 1,503 bp. Linkage analysis in an F3 population of 118 plants derived from the cross between the pair of NILS NIL7-2A (nod) × NIL7-2B (non-nod) revealed a co-localization between CaNSP2 and Rn1 gene. These data implicate the CaNSP2 gene as a candidate for identity to Rn1, and suggest that it could act in the nodulation signaling transduction pathway similarly to that in other legumes species.
KeywordsCicer Arietinum Nodulation Pathway Chickpea Genome Nodulin Gene GRAS Domain
This work has been supported by the project INIA contract RTA2010-00059, co-financed by EU funds (FEDER). We are also indebted to Dr. F. Temprano (IFAPA, Spain) for his support with Rhizobium inoculations. Ali L acknowledges PhD fellowship from Syrian Ministry of High Education and ICRISAT for supporting 6 months stay at the Center of Excellence in Genomics, ICRISAT, Patancheru, India (http://www.icrisat.org/ceg).
Conflict of interest
The authors declare that they have no conflict of interest.
- Arrighi JF, Barre A, Ben Amor B, Bersoult A, Soriano LC, Mirabella R, de Carvalho-Niebel F, Journet EP, Ghérardi M, Huguet T, Geurts R, Dénarié J, Rougé P, Gough C (2006) The Medicago truncatula lysine motif-receptor-like kinase gene family includes NFP and new nodule-expressed genes. J Plant Physiol 142:265–279. doi: 10.1104/pp.106.084657 CrossRefGoogle Scholar
- Davis TM (1988) Two genes that confer ineffective nodulation in chickpea (Cicer arietinum L.). J Hered 79:476–478Google Scholar
- Drummond AJ, Ashton B, Cheung M, Heled J, Kearse M, Moir R, Stones-Havas S, Thierer T, Wilson A (2010) Geneious v5.0. Available from http://www.geneious.com
- FAOSTAT (2013) http://faostat.fao.org. Last update January 2013
- Kalendar R, Lee D, Schulman AH (2009) FastPCR software for PCR primer and probe design and repeat search. Genes Genom Genomics 3:1–14Google Scholar
- Kalo P, Gleason C, Edwards A, Marsh J, Mitra RM, Hirsch S, Jakab J, Sims S, Long SR, Rogers J, Kiss GB, Downie JA, Oldroyd GED (2005) Nodulation signaling in legumes requires NSP2, a member of the GRAS family of transcriptional regulators. Science 308:1786–1789. doi: 10.1126/science.1110951 PubMedCrossRefGoogle Scholar
- Manickavelu A, Kawaura K, Oishi K, Shin IT, Kohara Y, Yahiaoui N, Keller B, Suzuki A, Yano K, Ogihara Y (2010) Comparative gene expression analysis of susceptible and resistant near-isogenic lines in common wheat infected by Puccinia triticina. DNA Res 17:211–222. doi: 10.1093/dnares/dsq009 PubMedCentralPubMedCrossRefGoogle Scholar
- Murakami Y, Miwa H, Imaizumi-Anraku H, Kouchi H, Downie JA, Kawaguchi M, Kawasaki S (2007) Positional cloning identifies Lotus japonicus NSP2, a putative transcription factor of the GRAS family, required for NIN and ENOD40 gene expression in nodule initiation. DNA Res 13:255–265. doi: 10.1093/dnares/dsl017 CrossRefGoogle Scholar
- Nayak S, Zhu H, Varghese N, Datta S, Choi H-K, Horres R, Jüngling R, Singh J, Kavi Kishor PB, Sivaramakrishnan S, Hoisington D, Kahl G, Winter P, Cook D, Varshney R (2010) Integration of novel SSR and gene-based SNP marker loci in the chickpea genetic map and establishment of new anchor points with Medicago truncatula genome. Theor Appl Genet 120:1415–1441. doi: 10.1007/s00122-010-1265-1 PubMedCentralPubMedCrossRefGoogle Scholar
- Seres A, Deák G, Tóth G, Aubert G, Burstin J, Ellis N, Kiss GB (2007) Comparative mapping. In: Mathesius U, Journet EP, Sumner LW (eds) The Medicago truncatula handbook. ISBN 0-9754303-1-9. http://www.noble.org/MedicagoHandbook/
- Van Ooijen JW (2006) MAPQIL® 5, software for the mapping of quantitative trait loci in experimental populations. Plant Research International, WagenningenGoogle Scholar
- Varshney RK, Song C, Saxena RK, Azam S et al (2013) Draft genome sequence of chickpea (Cicer arietinum) provides a resource for trait improvement. Nat Biotechnol. http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt.2491.html#supplementary-information
- Winter P, Pfaff T, Udupa SM, Hüttel B, Sharma PC, Sahi S, Arreguin-Espinoza R, Weigand F, Muehlbauer FJ, Kahl G (1999) Characterization and mapping of sequence-tagged microsatellite sites in the chickpea (Cicer arietinum L.) genome. Mol Gen Genet 262:90–101. doi: 10.1007/s004380051063 PubMedCrossRefGoogle Scholar
- Zuckercandl E, Pauling L (1965) Evolutionary divergence and convergence in proteins. In: Bryson V, Vogel HJ (eds) Evolving genes and proteins. Academic Press, USA, pp 97–165 (article-id:2345322)Google Scholar