Molecular mapping of stripe rust resistance gene Yr51 in chromosome 4AL of wheat
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This manuscript describes the chromosomal location of a new source of stripe rust resistance in wheat. DNA markers closely linked with the resistance locus were identified and validated.
A wheat landrace, AUS27858, from the Watkins collection showed high levels of resistance against Australian pathotypes of Puccinia striiformis f. sp. tritici. It was reported to carry two genes for stripe rust resistance, tentatively named YrAW1 and YrAW2. One hundred seeds of an F3 line (HSB#5515; YrAW1yrAW1) that showed monogenic segregation for stripe rust response were sown and harvested individually to generate monogenically segregating population (MSP) #5515. Stripe rust response variation in MSP#5515 conformed to segregation at a single locus. Bulked segregant analysis using high-throughput DArT markers placed YrAW1 in chromosome 4AL. MSP#5515 was advanced to F6 and phenotyped for detailed mapping. Novel wheat genomic resources including chromosome-specific sequence and genome zipper were employed to develop markers specific for the long arm of chromosome 4A. These markers were used for further saturation of the YrAW1 carrying region. YrAW1 was delimited by 3.7 cM between markers owm45F3R3 and sun104. Since there was no other stripe rust resistance gene located in chromosome 4AL, YrAW1 was formally named Yr51. Reference stock for Yr51 was lodged at the Australian Winter Cereal Collection, Tamworth, Australia and it was accessioned as AUS91456. Marker sun104 was genotyped on a set of Australian and Indian wheat cultivars and was shown to lack the resistance-linked sun104-225 bp allele. Marker sun104 is currently being used for marker-assisted backcrossing of Yr51 in Australian and Indian wheat backgrounds.
KeywordsSimple Sequence Repeat Marker Stripe Rust Recombinant Inbred Line Population DArT Marker Bulk Segregant Analysis
The first author thanks the Australian Centre for International Agricultural Research (ACIAR) for the award of John Allwright Fellowship to pursue Ph.D. study. We acknowledge financial support from Grant Agency of Czech Republic (project GACR P501/10/1740), Internal Grant Agency PrF-2012-001 and the Grains Research Development Corporation (GRDC) Australia. We are thankful to Diversity Arrays Technology Pty Ltd, Australia for sharing sequence information of clones.
- Akbari M, Wenzl P, Caig V, Carling J, Xia L, Yang S, Uszynski G, Mohler V, Lehmensiek A, Kuchel H, Hayden MJ, Howes N, Sharp P, Vaughan P, Rathmell B, Huttner E, Kilian A (2006) Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome. Theor Appl Genet 113:1409–1420PubMedCrossRefGoogle Scholar
- Bariana HS (2003) Breeding for disease resistance. In: Thomas B, Murphy DJ, Murray BG (eds) Encyclopedia of applied plant sciences. Harcourt, Academic Press, pp 244–253Google Scholar
- Berkman PJ, Skarshewski A, Manoli S, Lorenc MT, Stiller J, Smits L, Lai K, Campbell E, Kubaláková M, Simková H, Batley J, Doležel J, Hernandez P, Edwards D (2012) Sequencing wheat chromosome arm 7BS delimits the 7BS/4AL translocation and reveals homoeologous gene conservation. Theor Appl Genet 124:423–432PubMedCrossRefGoogle Scholar
- Hernandez P, Martis M, Dorado G, Pfeifer M, Galvez S, Schaaf S, Jouve N, Simkova H, Valarik M, Dolezel J, Mayer KFX (2012) Next-generation sequencing and syntenic integration of flow-sorted arms of wheat chromosome 4A exposes the chromosome structure and gene content. Plant J 69:377–386PubMedCrossRefGoogle Scholar
- Jakobson I, Reis D, Tiidema A, Peusha H, Timofejeva L, Valárik M, Kladivová M, Šimková H, Doležel J, Järve K (2012) Fine mapping, phenotypic characterization and validation of non-race-specific resistance to powdery mildew in a wheat-Triticum militinae introgression line. Theor Appl Genet 125:609–623PubMedCrossRefGoogle Scholar
- McIntosh RA, Dubcovsky J, Rogers J, Morris C, Appels R, Xia X (2011) Catalogue of gene symbols for wheat: 2011 supplementGoogle Scholar
- Miftahudin RK, Ma XF, Mahmoud AA, Layton J, Milla MA, Chikmawati T, Ramalingam J, Feril O, Pathan MS, Momirovic GS, Kim S, Chema K, Fang P, Haule L, Struxness H, Birkes J, Yaghoubian C, Skinner R, McAllister J, Nguyen V, Qi LL, Echalier B, Gill BS, Linkiewicz AM, Dubcovsky J, Akhunov ED, Dvorák J, Dilbirligi M, Gill KS, Peng JH, Lapitan NL, Bermudez-Kandianis CE, Sorrells ME, Hossain KG, Kalavacharla V, Kianian SF, Lazo GR, Chao S, Anderson OD, Gonzalez-Hernandez J, Conley EJ, Anderson JA, Choi DW, Fenton RD, Close TJ, McGuire PE, Qualset CO, Nguyen HT, Gustafson JP (2004) Analysis of expressed sequence tag loci on wheat chromosome group 4. Genetics 168:651–663PubMedCrossRefGoogle Scholar
- Sourdille P, Singh S, Cadalen T, Brown-Guedira GL, Gay G, Qi L, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genomics 4:12–25PubMedCrossRefGoogle Scholar
- Wicker T, Mayer KFX, Gundlach H, Mihaela M, Burkhard S, Uwe S, Simkova H, Kubalakova M, Choulet F, Taudien S, Platzer M, Feuillet C, Fahima T, Budak H, Dolezel Y, Keller B, Stein N (2011) Frequent genetic movement and pseudogene evolution is common to the large and complex genomes of wheat, barley, and their relatives. Plant Cell 23:1706–1718PubMedCentralPubMedCrossRefGoogle Scholar