Fine mapping of the Rrs1 resistance locus against scald in two large populations derived from Spanish barley landraces
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In two Spanish barley landraces with outstanding resistance to scald, the Rrs1 Rh4 locus was fine mapped including all known markers used in previous studies and closely linked markers were developed.
Scald, caused by Rhynchosporium commune, is one of the most prevalent barley diseases worldwide. A search for new resistance sources revealed that Spanish landrace-derived lines SBCC145 and SBCC154 showed outstanding resistance to scald. They were crossed to susceptible cultivar Beatrix to create large DH-mapping populations of 522 and 416 DH lines that were scored for disease resistance in the greenhouse using two R. commune isolates. To ascertain the pattern of resistance, parents and reference barley lines with known scald resistance were phenotyped with a panel of differential R. commune isolates. Subpopulations were genotyped with the Illumina GoldenGate 1,536 SNP Assay and a large QTL in the centromeric region of chromosome 3H, known to harbour several scald resistance genes and/or alleles, was found in both populations. Five SNP markers closest to the QTL were converted into CAPS markers. These CAPS markers, together with informative SSR markers used in other scald studies, confirmed the presence of the Rrs1 locus. The panel of differential scald isolates indicated that the allele carried by both donors was Rrs1 Rh4 . The genetic distance between Rrs1 and its flanking markers was 1.2 cM (11_0010) proximally and 0.9 cM (11_0823) distally, which corresponds to a distance of just below 9 Mbp. The number and nature of scald resistance genes on chromosome 3H are discussed. The effective Rrs1 allele found and the closely linked markers developed are already useful tools for molecular breeding programs and provide a good step towards the identification of candidate genes.
KeywordsDouble Haploid Resistance Locus Double Haploid Line Double Haploid Population Commune Isolate
Spanish barley core collection
South Australian Research and Development Institute
Barley oligonucleotid pool assay
This work was funded by the German BMELV and BLE through the project “Rhynchosporium secalis-resistance” in barley (project 28-1-41.009-06) and PLANT KBBE II “ExpResBar” AZ 0315702C and by the Spanish Ministry of Science and Innovation (projects GEN2006-28560-E, AGL2007-63625, RFP2012-00015-00-00 and PLANT KBBE “ExpResBar”, EUI2009-04075) and co-funded by the European Regional Development Fund. CS held an I3P contract from CSIC. BCM is supported by Fundación ARAID, Zaragoza, Spain. Barley-SSR markers were kindly provided by Andreas Graner and Marion Röder, IPK Gatersleben. Isolates UK7 and AU2 were kindly provided by W. Knogge, Leibnitz Institute for Plant Biochemistry, Halle. The authors would like to thank Alfred Barth and Alexandra Jestadt for the high level of commitment in the laboratory, greenhouse and field and Carlos P. Cantalapiedra for assistance in the work with the physical map of barley. The Grains Research and Development Corporation (GRDC) and SARDI funded the work in Australia.
Conflict of interest
The authors declare that they have no conflict of interest.
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