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Theoretical and Applied Genetics

, Volume 126, Issue 10, pp 2467–2475 | Cite as

The Pontin series of recombinant alien translocations in bread wheat: single translocations integrating combinations of Bdv2, Lr19 and Sr25 disease-resistance genes from Thinopyrum intermedium and Th. ponticum

  • L. I. Ayala-Navarrete
  • A. A. Mechanicos
  • J. M. Gibson
  • D. Singh
  • H. S. Bariana
  • J. Fletcher
  • S. Shorter
  • Philip J. LarkinEmail author
Original Paper

Abstract

Two bread wheat lines each with a translocation on chromosome 7DL from either Thinopyrum intermedium (TC5 and TC14) or Thinopyrum ponticum (T4m), were hybridized in a ph1b mutant background to enhance recombination between the two translocated chromosomal segments. The frequency of recombinants was high in lines derived from the larger and similar-sized translocations (TC5/T4m), but much lower when derived from different-sized translocations (TC14/T4m). Recombinant translocations contained combinations of resistance genes Bdv2, Lr19 and Sr25 conferring resistance to Barley yellow dwarf virus (BYDV), leaf rust and stem rust, respectively. Their genetic composition was identified using bioassays and molecular markers specific for the two progenitor Thinopyrum species. This set of 7DL Th. ponticum/intermedium recombinant translocations was termed the Pontin series. In addition to Thinopyrum markers, the size of the translocation was estimated with the aid of wheat markers mapped on each of the 7DL deletion bins. Bioassays for BYDV, leaf rust and stem rust were performed under greenhouse and field conditions. Once separated from ph1b background, the Pontin recombinant translocations were stable and showed normal inheritance in successive backcrosses. The reported Pontin translocations integrate important resistance genes in a single linkage block which will allow simultaneous selection of disease resistance. Combinations of Bdv2 + Lr19 or Lr19 + Sr25 in both long and short translocations, are available to date. The smaller Pontins, comprising only 20 % of the distal portion of 7DL, will be most attractive to breeders.

Keywords

Leaf Rust Stem Rust Barley Yellow Dwarf Virus Leaf Rust Resistance Gene Homoeologous Recombination 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors gratefully acknowledge the partial support from the Australian Grains Research and Development Corporation for grant CSP00090 and Dr Ian Dundas for the Angus ph1b germplasm. We thank Dr Hanif Miah for excellent technical assistance.

Conflict of interest

The authors declare that they have no other conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • L. I. Ayala-Navarrete
    • 1
    • 3
  • A. A. Mechanicos
    • 1
  • J. M. Gibson
    • 1
  • D. Singh
    • 2
    • 4
  • H. S. Bariana
    • 2
  • J. Fletcher
    • 5
  • S. Shorter
    • 5
  • Philip J. Larkin
    • 1
    Email author
  1. 1.CSIRO Plant IndustryCanberra ACTAustralia
  2. 2.Plant Breeding Institute-Cobbitty, Faculty of Agriculture and Environment, University of SydneyNarellanAustralia
  3. 3.Escuela Politécnica del Ejercito (ESPE)SangolquiEcuador
  4. 4.CIMMYT Global Wheat ProgramNairobiKenya
  5. 5.New Zealand Institute for Plant and Food ResearchLincolnNew Zealand

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