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Theoretical and Applied Genetics

, Volume 126, Issue 8, pp 2177–2185 | Cite as

Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI 567139B

  • Feng Lin
  • Meixia Zhao
  • Jieqing Ping
  • Austin Johnson
  • Biao Zhang
  • T. Scott Abney
  • Teresa J. HughesEmail author
  • Jianxin MaEmail author
Original Paper

Abstract

Phytophthora root and stem rot (PRR), caused by the soil-borne oomycete pathogen Phytophthora sojae, is one of the most destructive diseases of soybean. PRR can be effectively controlled by race-specific genes conferring resistance to P. sojae (Rps). However, the Rps genes are usually non-durable, as populations of P. sojae are highly diverse and quick to adapt, and can be overcome 8–15 years after deployment. Thus, it is important to identify novel Rps genes for development of resistant soybean cultivars. PI 567139B is a soybean landrace carrying excellent resistance to nearly all predominant P. sojae races in Indiana. A mapping population consisting of 245 F2 individuals and 403 F2:3 families was developed from a cross between PI 567139B and the susceptible cultivar ‘Williams’, and used to dissect the resistance carried by PI 567139B. We found that the resistance in PI 567139B was conferred by two independent Rps genes, designated RpsUN1 and RpsUN2. The former was mapped to a 6.5 cM region between SSR markers Satt159 and BARCSOYSSR_03_0250 that spans the Rps1 locus on chromosome 3, while the latter was mapped to a 3.0 cM region between BARCSOYSSR_16_1275 and Sat_144, approximately 3.0–3.4 cM upstream of Rps2 on chromosome 16. According to the ‘Williams 82’ reference genome sequence, both regions are highly enriched with NBS-LRR genes. Marker assisted resistance spectrum analyses of these genes with 16 isolates of P. sojae, in combination with the mapping results, suggested that RpsUN1 was likely to be a novel allele at the Rps1 locus, while RpsUN2 was more likely to be a novel Rps gene.

Keywords

Simple Sequence Repeat Marker Cetyl Trimethyl Ammonium Bromide Reference Genome Sequence Soybean Line Soybean Chromosome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was mainly supported by soybean checkoff funds from the Indiana Soybean Alliance (Grant No. 205267), and partially supported by Agricultural Alumni Seed Improvement Association and the Purdue University Agricultural Research Program. We also thank Elisa Hughes, Brittany Radke, Tomara J. Fleury and Brian Foss for their help in inoculation and preparation of inoculum.

Supplementary material

122_2013_2127_MOESM1_ESM.xlsx (61 kb)
Genotype and reaction pattern of 403 F2:3 mapping families to isolates pmg(17)-1 and pmg(25)-1 of P. sojae. Supplementary material 1 (XLSX 61 kb)
122_2013_2127_MOESM2_ESM.xlsx (47 kb)
Marker assisted resistance spectrum analysis of RpsUN1 and RpsUN2 using selected F2:3 families. Supplementary material 2 (XLSX 46 kb)
122_2013_2127_MOESM3_ESM.xlsx (15 kb)
Allelism tests of RpsUN1 and Rps1-k. Supplementary material 3 (XLSX 15 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Feng Lin
    • 1
  • Meixia Zhao
    • 1
  • Jieqing Ping
    • 1
  • Austin Johnson
    • 1
  • Biao Zhang
    • 1
  • T. Scott Abney
    • 2
    • 3
  • Teresa J. Hughes
    • 3
    Email author
  • Jianxin Ma
    • 1
    Email author
  1. 1.Department of AgronomyPurdue UniversityWest LafayetteUSA
  2. 2.Department of Botany and Plant PathologyPurdue UniversityWest LafayetteUSA
  3. 3.USDA-ARS Crop Production and Pest Control Research UnitPurdue UniversityWest LafayetteUSA

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