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Radiation hybrid QTL mapping of Tdes2 involved in the first meiotic division of wheat

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Abstract

Since the dawn of wheat cytogenetics, chromosome 3B has been known to harbor a gene(s) that, when removed, causes chromosome desynapsis and gametic sterility. The lack of natural genetic diversity for this gene(s) has prevented any attempt to fine map and further characterize it. Here, gamma radiation treatment was used to create artificial diversity for this locus. A total of 696 radiation hybrid lines were genotyped with a custom mini array of 140 DArT markers, selected to evenly span the whole 3B chromosome. The resulting map spanned 2,852 centi Ray with a calculated resolution of 0.384 Mb. Phenotyping for the occurrence of meiotic desynapsis was conducted by measuring the level of gametic sterility as seeds produced per spikelet and pollen viability at booting. Composite interval mapping revealed a single QTL with LOD of 16.2 and r 2 of 25.6 % between markers wmc326 and wPt-8983 on the long arm of chromosome 3B. By independent analysis, the location of the QTL was confirmed to be within the deletion bin 3BL7-0.63-1.00 and to correspond to a single gene located ~1.4 Mb away from wPt-8983. The meiotic behavior of lines lacking this gene was characterized cytogenetically to reveal striking similarities with mutants for the dy locus, located on the syntenic chromosome 3 of maize. This represents the first example to date of employing radiation hybrids for QTL analysis. The success achieved by this approach provides an ideal starting point for the final cloning of this interesting gene involved in meiosis of cereals.

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Abbreviations

3B-RH:

Radiation hybrids for chromosome 3B

cR:

Centi Rays

CS:

Chinese Spring

DArT:

Diversity Arrays Technology

Gy:

Gray

H 2 :

Broad sense heritability

LDN:

Langdon

LDN 3D (3B):

Langdon substitution line 3D for 3B

M1 :

Mutant generation 1

MS:

Mean sum of squares

MSg:

Mean sum of squares for the genotype

MSge:

Mean sum of squares for the genotype by environment

PV:

Pollen viability

QTL:

Quantitative trait loci

RH0 or RH1 :

Radiation hybrid generation 0 or 1

SpS:

Seeds per spikelet

SSR:

Single sequence repeat

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Acknowledgments

The authors wish to thank Professor Wojtek Pawlowski (Cornell University, NY) for in-depth revision and insightful comments on this manuscript, Justin Hegstad and Allen Peckrul for their qualified technical help. This work was supported by funding from the National Science Foundation, Plant Genome Research Program (NSF-PGRP) grant No. IOS-0822100 to SFK. F.M.B was partially supported by Program Master and Back Regione Autonoma della Sardegna and Monsanto Beachell-Borlaug International Scholarship.

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Authors and Affiliations

  1. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58102, USA

    F. M. Bassi, A. Kumar, Q. Zhang, R. Dizon & S. F. Kianian

  2. International Center for the Agricultural Research in the Dry Areas, Rabat, 10112, Morocco

    F. M. Bassi

  3. Genetics Diversity and Ecophysiology of Cereals, UMR INRA-UBP 1095, Clermont Ferrand, 63039, France

    E. Paux & C. Feuillet

  4. Australian Centre for International Agricultural Research, GPO Box 1571, Canberra, ACT, 2601, Australia

    E. Huttner

  5. Diversity Arrays Technology Pty Ltd., Yarralumla, ACT, 2600, Australia

    A. Kilian & S. S. Xu

  6. ARS Cereal Crops Research Unit, USDA, Fargo, ND, 58102, USA

    S. S. Xu

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  1. F. M. Bassi
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  2. A. Kumar
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  3. Q. Zhang
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  8. C. Feuillet
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  10. S. F. Kianian
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Correspondence to S. F. Kianian.

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Communicated by P. Heslop-Harrison.

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Bassi, F.M., Kumar, A., Zhang, Q. et al. Radiation hybrid QTL mapping of Tdes2 involved in the first meiotic division of wheat. Theor Appl Genet 126, 1977–1990 (2013). https://doi.org/10.1007/s00122-013-2111-z

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  • DOI: https://doi.org/10.1007/s00122-013-2111-z

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