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Genetic analysis and mapping of genes for resistance to multiple strains of Soybean mosaic virus in a single resistant soybean accession PI 96983

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Abstract

Soybean mosaic virus (SMV) is one of the most broadly distributed soybean (Glycine max (L.) Merr.) diseases and causes severe yield loss and seed quality deficiency. Multiple studies have proved that a single dominant gene can confer resistance to several SMV strains. Plant introduction (PI) 96983 has been reported to contain SMV resistance genes (e.g., Rsv1 and Rsc14) on chromosome 13. The objective of this study was to delineate the genetics of resistance to SMV in PI 96983 and determine whether one gene can control resistance to more than one Chinese SMV strain. In this study, PI 96983 was identified as resistant and Nannong 1138-2 was identified as susceptible to four SMV strains SC3, SC6, SC7, and SC17. Genetic maps based on 783 F2 individuals from the cross of PI 96983 × Nannong 1138-2 showed that the gene(s) conferring resistance to SC3, SC6, and SC17 were between SSR markers BARCSOYSSR_13_1114 and BARCSOYSSR_13_1136, whereas SC7 was between markers BARCSOYSSR_13_1140 and BARCSOYSSR_13_1185. The physical map based on 58 recombinant lines confirmed these results. The resistance gene for SC7 was positioned between BARCSOYSSR_13_1140 and BARCSOYSSR_13_1155, while the resistance gene(s) for SC3, SC6, and SC17 were between BARCSOYSSR_13_1128 and BARCSOYSSR_13_1136. We concluded that, there were two dominant resistance genes flanking Rsv1 or one of them at the reported genomic location of Rsv1. One of them (designated as “Rsc-pm”) conditions resistance for SC3, SC6, and SC17 and another (designated as “Rsc-ps”) confers resistance for SC7. The two tightly linked genes identified in this study would be helpful to cloning of resistance genes and breeding of multiple resistances soybean cultivars to SMV through marker-assisted selection (MAS).

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References

  1. Chen P, Choi CW (2007) Characterization of genetic interaction between soybean and soybean mosaic virus. In: Rao GP (ed) Molecular diagnosis of plant viruses. Studium Press, Houston, TX, pp 389–422

  2. Chen P, Buss GR, Roane CW, Tilin SA (1991) Allelism among genes for resistance to soybean mosaic virus in strain-differential soybean cultivars. Crop Sci 31:305–309

  3. Cho ER, Goodman RM (1979) Strains of soybean mosaic virus: classification based on virulence in resistant soybean cultivars. Phytopathology 69:467–470

  4. Cho ER, Goodman RM (1982) Evaluation of resistance in soybeans to soybean mosaic virus strains. Crop Sci 22:1133–1136

  5. Cregan PB, Jarvik T, Bush AL, Shoemaker RC, Lark KG, Kahler AL, Kaya N, VanToai TT, Lohnes DG, Chung J, Specht JE (1999) An integrated genetic linkage map of the soybean. Crop Sci 39:1464–1490

  6. Ellis JG, Lawrence GJ, Finnegan EJ, Anderson PA (1995) Contrasting complexity of two rust resistance loci in flax. Proc Natl Acad Sci USA 92:4185–4188

  7. Fu SX, Zhan Y, Zhi HJ, Gai JY, Yu DY (2006) Mapping of SMV resistance gene Rsc7 by SSR markers in soybean. Genetica 128:63–69

  8. Gore MA, Hayes AJ, Jeong SC, Yue YG, Buss GR, Saghai Maroof MA (2002) Mapping tightly linked genes controlling potyvirus infection at the Rsv1 and Rpv1 region in soybean. Genome 45:592–599

  9. Guo DQ, Zhi HJ, Wang YW, Gai JY, Zhou XA, Yang CL (2005) Identification and distribution of strains of soybean mosaic virus in middle and northern of HuangHuai Region of China. Soybean Sci 27:64–68

  10. Hatch E, Lazaraton E (1991) The research manual: Design and statistics for applied linguistics. Newbury/HarperCollins, New York

  11. Hayes AJ, Saghai Maroof MA (2000) Targeted resistance gene mapping in soybean using modified AFLPs. Theor Appl Genet 100:1279–1283

  12. Hayes AJ, Ma GR, Buss GR, Saghai Maroof MA (2000) Molecular marker mapping of Rsv4, agene conferring resistance to all known strains of soybean mosaic virus. Crop Sci 40:1434–1437

  13. Hayes AJ, Jeong SC, Gore MA, Yu YG, Buss GR, Tolin SA, Saghai Maroof MA (2004) Recombination with in a nucleotide-binding-site/leucine-rich-repeat gene cluster produces new variants conditioning resistance to soybean mosaic virus in soybeans. Genetics 166:493–503

  14. Jeong SC, Saghai Maroof MA (2004) Detection and genotyping of SNPs tightly linked to two disease resistance loci, Rsv1 and Rsv3, of soybean. Plant Breed 123:305–310

  15. Jeong SC, Kristipati S, Hayes AJ, Maughan PJ, Noffsinger SL, Gunduz I, Buss GR, Saghai Maroof MA (2002) Genetic and sequence analysis of markers tightly linked to the soybean mosaic virus resistance gene, Rsv3. Crop Sci 42:265–270

  16. Kiihl RAS, Hartwig EE (1979) Inheritance of reaction to soybean mosaic virus in soybeans. Crop Sci 19:372–375

  17. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

  18. Li HC, Zhi HJ, Gai JY, Guo DQ, Wang YW, Li K, Bai L, Yang H (2006) Inheritance and gene mapping of resistance to soybean mosaic virus strain SC14 in soybean. J Integr Plant Biol 48:1466–1472

  19. Li K, Yang QH, Zhi HJ, Gai JY, Yu DY (2010) Identification and distribution of soybean mosaic virus strains in southern China. Plant Dis 94:351–357

  20. Liao L, Chen P, Rajcan I, Buss GR, Tolin SA (2011) Genetic analysis of “8101” soybean containing three genes for resistance to soybean mosaic virus. Crop Sci 51:503–511

  21. Liu RH, Meng JL (2003) Map Draw: a Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Hered 25(3):317–321

  22. Ma Y, Li HC, Wang DG, Liu N, Zhi HJ (2010) Molecular mapping and marker assisted selection of soybean mosaic virus resistance gene RSC12 in soybean. Legum Genomics Genet 8:41–46

  23. Ma Y, Wa DG, Li HC, Zheng GJ, Yang YQ, Li HW, Zhi HJ (2011) Fine mapping of the Rsc14Q locus for resistance to soybean mosaic virus in soybean. Euphytica 181(1):127–135

  24. Meng Z, Zaykin DV, Xu CF, Wagner M, Ehm MG (2003) Selection of genetic markers for association analyses, using linkage disequilibrium and haplotypes. Am J Hum Genet 73:115–130

  25. Meyers BC, Chin DB, Shen KA, Sivaramakrishnan S, Lavelle DO, Zhang Z, Michelmore RW (1998) The major resistance gene cluster in lettuce is highly duplicated and spans several megabases. Plant Cell 10:1817–1832

  26. Mudge J, Cregan PB, Kenworthy JP, Kenworthy WJ, Orf JH, Young ND (1997) Two micro satellite markers that flank the major soybean cyst nematode resistance locus. Crop Sci 37:1611–1615

  27. Parniske M, Jones JDG (1999) Recombination between diverged clusters of the tomato Cf-9 plant disease resistance gene family. Proc Natl Acad Sci USA 96:5850–5855

  28. Saghai Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci 81:8014–8018

  29. Saghai Maroof MA, Tucker DM, Skoneczka JA, Bowman BC, Tripathy S, Tolin SA (2010) Fine mapping and candidate gene discovery of the soybean mosaic virus resistance gene, Rsv4. Plant genome 3:14–22

  30. Song WY, Pi LY, Wang GL, Gardner J, Holsten T, Ronald PC (1997) Evolution of the rice Xa21 disease resistance gene family. Plant Cell 9:1279–1287

  31. Song QJ, Marek LF, Shoemaker RC, Lark KG, Concibido VC, Delannay X, Specht JE, Cregan PB (2004) A new integrated genetic linkage map of the soybean. Theor Appl Genet 109(1):122–128

  32. Song QJ, Jia GF, Zhu YL, Grant D, Nelson RT, Hwang EY, Hyten DL, Cregan PB (2010) Abundance of SSR motifs and development of candidate polymorphic SSR markers (BARCSOYSSR_1.0) in soybean. Crop Sci 50:1950–1960

  33. Van Ooijen JW, Voorrips RE (2001) JoinMap(R) 3.0, Software for the calculation of genetic linkage maps. Plant Research International, Wageningen

  34. Wang XQ, Gai JY, Pu ZQ (2003) Classification and distribution of strains of soybean mosaic virus in middle and lower Huanghuai and Changjiang river valleys. Soybean Sci 22:102–107

  35. Wang YW, Zhi HJ, Guo DQ, Gai JY, Chen QS, Li K, Li HC (2005) Classification and distribution of strains of Soybean mosaic virus in northern China spring planting soybean region. Soybean Sci 24:263–268

  36. Wang DG, Ma Y, Yang YQ, Liu N, Li Song YP, Zhi HJ (2011a) Fine mapping and analyses of RSC8 resistance candidate genes to soybean mosaic virus in soybean. Theor Appl Genet 122:555–565

  37. Wang DG, Ma Y, Liu N, Yang ZL, Zheng GJ, Zhi HJ (2011b) Fine mapping of resistance to soybean mosaic virus strain SC4 in soybean based on genomic-SSR markers. Plant Breed 130:653–659

  38. Yang QH, Gai JY (2010) Identification, inheritance and gene mapping of resistance to avirulent soybean mosaic virus strain SC15 in soybean. Plant Breed. doi:10.1111/j.1439-0523.2010.01797.x

  39. Yates F (1934) Contingency table involving small numbers and the χ2 test. Suppl J r Stat Soc 1(2):217–235

  40. Yu YG, Saghai Maroof MA, Buss GR, Maughan PJ, Tolin SA (1994) RFLP and micro satellite mapping of a gene for soybean mosaic virus resistance. Phytopathol 84:60–64

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (GrantNo.30971815, 31171574), the National Soybean Industrial Technology System of China (No. CARS-004) and the Fund of Transgenic Breeding for Soybean Resistance to Soybean Mosaic Virus (No.2008ZX08004-004). Our sincere thanks also extended to Mr. Song Q.J. from Soybean Genomics and Improvement Laboratory, USDA-ARS for his providing the SSR markers primer sequences.

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Correspondence to Haijian Zhi.

Additional information

Y. Yang and G. Zheng contributed equally to this study.

Communicated by J. Ray.

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Yang, Y., Zheng, G., Han, L. et al. Genetic analysis and mapping of genes for resistance to multiple strains of Soybean mosaic virus in a single resistant soybean accession PI 96983. Theor Appl Genet 126, 1783–1791 (2013). https://doi.org/10.1007/s00122-013-2092-y

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Keywords

  • Simple Sequence Repeat Marker
  • Plant Introduction
  • Recombinant Line
  • Soybean Mosaic Virus
  • Pymetrozine