QTL analysis of the spring wheat “Chapio” identifies stable stripe rust resistance despite inter-continental genotype × environment interactions
- 971 Downloads
Chapio is a spring wheat developed by CIMMYT in Mexico by a breeding program that focused on multigenic resistances to leaf rust and stripe rust. A population consisting of 277 recombinant inbred lines (RILs) was developed by crossing Chapio with Avocet. The RILs were genotyped with DArT markers (137 randomly selected RILs) and bulked segregant analysis conducted to supplement the map with informative SSR markers. The final map consisted of 264 markers. Phenotyping against stripe rust was conducted for three seasons in Toluca, Mexico and at three sites over two seasons (total of four environments) in Sichuan Province, China. Significant loci across the two inter-continental regions included Lr34/Yr18 on 7DS, Sr2/Yr30 on 3BS, and a QTL on 3D. There were significant genotype × environment interactions with resistance gene Yr31 on 2BS being effective in most of the Toluca environments; however, a late incursion of a virulent pathotype in 2009 rendered this gene ineffective. This locus also had no effect in China. Conversely, a 5BL locus was only effective in the Chinese environments. There were also complex additive interactions. In the Mexican environments, Yr31 suppressed the additive effect of Yr30 and the 3D locus, but not of Lr34/Yr18, while in China, the 3D and 5BL loci were generally not additive with each other, but were additive when combined with other loci. These results indicate the importance of maintaining diverse, multi-genic resistances as Chapio had stable inter-continental resistance despite the fact that there were QTLs that were not effective in either one or the other region.
KeywordsQuantitative Trait Locus Simple Sequence Repeat Marker Leaf Rust Quantitative Trait Locus Analysis Stripe Rust
The study was supported by The Ministry of Science and Technology of China (Grant No. 2011DFG33350), Science & Technology Department of Sichuan Province (Grant No. 2010HH0018, 2011JQ0036, 2012JQ0013) and the Grains Research and Development Council of Australia (CIM00013 and CIM00015). Thanks to Ky Mathews for providing statistical support.
Conflict of interest
The authors declare that there is no conflict of interest.
- Falk CT (1989) A simple scheme for preliminary ordering of multiple loci: application to 45 CF families. In: Elston RC, Spence MA, Hodge SE, MacCluer JW (eds) Multipoint mapping and linkage based upon affected pedigree members. Genetic Workshop 6, New York, pp 17–22Google Scholar
- Hare RA, McIntosh M (1979) Genetic and cytogenetic studies of durable adult-plant resistances in Hope and related cultivars to wheat rusts. Z Planzenzücht 83:350–367Google Scholar
- Hoisington D, Khairallah M, Gonzalez-de-Leon D (1994) Laboratory protocols: CIMMYT applied molecular genetics laboratory, 2nd edn. CIMMYT, MexicoGoogle Scholar
- McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2011) Catalogue of gene symbols for wheat: 2011 supplement. http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2011.pdf
- Rosewarne GM, Singh RP, Huerta-Espino J, Herrera-Foessel SA, Forrest KL, Hayden MJ, Rebetzke GJ (2012) Analysis of leaf and stripe rust severities reveals pathotype changes and multiple minor QTLs associated with resistance in an Avocet × Pastor wheat population. Theor Appl Genet 124:1283–1294PubMedCrossRefGoogle Scholar
- Singh RP, Huerta-Espino J, Rajaram S (2000a) Achieving near-immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathol Hun 35:133–139Google Scholar
- Singh RP, William HM, Huerta-Espino J, Crosby M (2003) Identification and mapping of gene Yr31 for resistance to stripe rust in Triticum aestivum cultivar Pastor. In: Pogna NE, Romano N, Pogna EA, Galterio G (eds) Proceedings 10th international wheat genetics symposium, vol 1. Instituto Sperimentale per la Cerealcoltura, Rome, pp 411–413Google Scholar
- Yang J, Hu C-C, Ye X-Z, Zhu J (2005) QTLNetwork 2.0. Institute of Bioinformatics, Zhejiang University, Hangzhou, China. (http://ibi.zju.edu.cn/software/qtlnetwork)