Fine-mapping and molecular marker development for Pi56(t), a NBS-LRR gene conferring broad-spectrum resistance to Magnaporthe oryzae in rice
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The major quantitative trait locus qBR9.1 confers broad-spectrum resistance to rice blast, and was mapped to a ~69.1 kb region on chromosome 9 that was inherited from resistant variety Sanhuangzhan No 2 (SHZ-2). Within this region, only one predicted disease resistance gene with nucleotide binding site and leucine-rich repeat (NBS-LRR) domains was found. Specific markers corresponding to this gene cosegregated with blast resistance in F2 and F3 populations derived from crosses of susceptible variety Texianzhan 13 (TXZ-13) to SHZ-2 and the resistant backcross line BC-10. We tentatively designate the gene as Pi56(t). Sequence analysis revealed that Pi56(t) encodes an NBS-LRR protein composed of 743 amino acids. Pi56(t) was highly induced by blast infection in resistant lines SHZ-2 and BC-10. The corresponding allele of Pi56(t) in the susceptible line TXZ-13 encodes a protein with an NBS domain but without LRR domain, and it was not induced by Magnaporthe oryzae infection. Three new cosegregating gene-specific markers, CRG4-1, CRG4-2 and CRG4-3, were developed. In addition, we evaluated polymorphism of the gene-based markers among popular varieties from national breeding programs in Asia and Africa. The presence of the CRG4-2 SHZ-2 allele cosegregated with a blast-resistant phenotype in two BC2F1 families of SHZ-2 crossed to recurrent parents IR64-Sub1 and Swarna-Sub1. CRG4-1 and CRG4-3 showed clear polymorphism among 19 varieties, suggesting that they can be used in marker-assisted breeding to combine Pi56(t) with other target genes in breeding lines.
KeywordsQuantitative Trait Locus Rice Blast Blast Resistance Disease Reaction BC2F1 Population
This work was supported in part by grants from the Generation Challenge Program (HL), the MOST Key International Collaboration Project (2006DFB33320, BL, HL),NSFC-IRRI Project (30821140350, BL, HL), and the Guangdong International Collaboration Project (2007A050100037, BL).
- Hayashi N, Inoue H, Kato T, Funao T, Shirota M, Shimizu T, Kanamori H, Yamane H, Hayano- Saito Y, Matsumoto T, Yano M, Takatsuji H (2010) Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication. Plant J 64(3):498–510PubMedCrossRefGoogle Scholar
- Hittalmani S, Foolad MR, Mew T, Rodriguez RL, Huang N (1995) Development of a PCR-based marker to identify rice blast resistance gene, Pi-2(t), in a segregating population. Theor Appl Genet 109:978–985Google Scholar
- Huang H, Huang L, Feng G, Wang S, Wang Y, Liu J, Jiang N, Yan W, Xu L, Sun P, Liu Z, Pan S, Liu X, Xiao Y, Liu E, Dai L, Wang G (2010) Molecular mapping of the new blast resistance genes Pi47 and Pi48 in the durably resistant local rice cultivar Xiangzi 3150. Phytopathology 101(5):620–626CrossRefGoogle Scholar
- Okuyama Y, Kanzaki H, Abe A, Yoshida K, Tamiru M, Saitoh H, Fujibe T, Matsumura H, Shenton M, Galam DC, Undan J, Ito A, Sone T, Terauchi R (2011) A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes. Plant J 66(3):467–479PubMedCrossRefGoogle Scholar
- Roh JH, Kim BR, Lee SW, Cho YC, Ra DS, Oh IS, Han SS (2009) Sequential planting as a method of screening of durable resistance to rice blast in Korea. In: Wang GL, Valent B (eds) Advances in genetics, genomics and control of rice blast disease. Springer, Netherlands, pp 337–346CrossRefGoogle Scholar
- Sharma TR, Rai AK, Gupta GK, Singh NK (2010) Broad spectrum blast resistance gene Pikh cloned from the rice line Tetep designated as Pi54. J Plant Biochem Biotechnol 19(1):87–89Google Scholar