Multi-environment analysis and improved mapping of a yield-related QTL on chromosome 3B of wheat
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Improved mapping, multi-environment quantitative trait loci (QTL) analysis and dissection of allelic effects were used to define a QTL associated with grain yield, thousand grain weight and early vigour on chromosome 3BL of bread wheat (Triticum aestivum L.) under abiotic stresses. The QTL had pleiotropic effects and showed QTL x environment interactions across 21 diverse environments in Australia and Mexico. The occurrence and the severity of water deficit combined with high temperatures during the growing season affected the responsiveness of this QTL, resulting in a reversal in the direction of allelic effects. The influence of this QTL can be substantial, with the allele from one parent (RAC875) increasing grain yield by up to 12.5 % (particularly in environments where both heat and drought stress occurred) and the allele from the other parent (Kukri) increasing grain yield by up to 9 % in favourable environments. With the application of additional markers and the genotyping of additional recombinant inbred lines, the genetic map in the QTL region was refined to provide a basis for future positional cloning.
The authors thank Ali Izanloo for field data; Eugenio Perez, Araceli Torres and other members of the CIMMYT physiology group for data collection; Delphine Fleury and other members of the ACPFG for advice and technical support; and Pierre Sourdille (INRA Clermont-Ferrand) for marker sequences. The work was supported through funding from the Grain Research and Development Corporation, the Australian Research Council, the Government of South Australia and the University of Adelaide.
- Bennett D, Izanloo A, Edwards J, Kuchel H, Chalmers K, Tester M, Reynolds M, Schnurbusch T, Langridge P (2012c) Identification of novel quantitative trait loci for days to ear emergence and flag leaf glaucousness in a bread wheat (Triticum aestivum L.) population adapted to southern Australian conditions. Theor Appl Genet 124:697–711PubMedCrossRefGoogle Scholar
- Broman K, Wu H with ideas from Gary Churchill SS; Contributions from Brian Yandell (2010) qtl: tools for analysing QTL experiments. R package version 1.15-15Google Scholar
- Butler D, Cullis B, Gilmour A, Gogel B (2009) ASReml-R, reference manual. Technical report, Queensland Department of Primary IndustriesGoogle Scholar
- Cone KC, McMullen MD, Bi IV, Davis GL, Yim Y-S, Gardiner JM, Polacco ML, Sanchez-Villeda H, Fang Z, Schroeder SG, Havermann SA, Bowers JE, Paterson AH, Soderlund CA, Engler FW, Wing RA, Coe EH (2002) Genetic, physical, and informatics resources for maize. On the road to an integrated map. Plant Physiol 130:1598–1605PubMedCrossRefGoogle Scholar
- Deckers J, Verhulst N, Govaerts B (2009) Classification of the soil at CIMMYT’s experimental station in the Yaqui Valley near Ciudad Obregon, Sonora, Mexico. CIMMYT, Mexico, DFGoogle Scholar
- Diab AA, Kantety RV, Ozturk NZ, Benscher D, Nachit MM, Sorrells ME (2008) Drought-inducible genes and differentially expressed sequence tags associated with components of drought tolerance in durum wheat. Sci Res Essays 3:9–26Google Scholar
- Krattinger S, Wicker T, Keller B (2009) Map-based cloning of genes in Triticeae (wheat and barley). In: Muehlbauer GJ, Feuillet C (eds) Genetics and genomics of the Triticeae. Springer US, pp 337–357Google Scholar
- Maccaferri M, Sanguineti MC, Corneti S, Ortega JL, Salem MB, Bort J, DeAmbrogio E, del Moral LF, Demontis A, El-Ahmed A, Maalouf F, Machlab H, Martos V, Moragues M, Motawaj J, Nachit M, Nserallah N, Ouabbou H, Royo C, Slama A, Tuberosa R (2008) Quantitative trait loci for grain yield and adaptation of durum wheat (Triticum durum Desf.) across a wide range of water availability. Genetics 178:489–511PubMedCrossRefGoogle Scholar
- McCord AK, Payne RA (2004) Report on the condition of agricultural land in South Australia. Department of Water Land and Biodiversity Conservation, AdelaideGoogle Scholar
- McIntyre C, Mathews K, Rattey A, Chapman S, Drenth J, Ghaderi M, Reynolds M, Shorter R (2010) Molecular detection of genomic regions associated with grain yield and yield-related components in an elite bread wheat cross evaluated under irrigated and rainfed conditions. Theor Appl Genet 120:527–541PubMedCrossRefGoogle Scholar
- Paux E, Sourdille P, Salse J, Saintenac C, Choulet F, Leroy P, Korol A, Michalak M, Kianian S, Spielmeyer W, Lagudah E, Somers D, Kilian A, Alaux M, Vautrin S, Berges H, Eversole K, Appels R, Safar J, Simkova H, Dolezel J, Bernard M, Feuillet C (2008) A physical map of the 1-gigabase bread wheat chromosome 3B. Science 322:101–104PubMedCrossRefGoogle Scholar
- Paux E, Faure S, Choulet F, Roger D, Gauthier V, Martinant JP, Sourdille P, Balfourier F, Le Paslier MC, Chauveau A, Cakir M, Gandon B, Feuillet C (2010) Insertion site-based polymorphism markers open new perspectives for genome saturation and marker-assisted selection in wheat. Plant Biotechnol J 8:196–210PubMedCrossRefGoogle Scholar