Fine genetic mapping of RXopJ4, a bacterial spot disease resistance locus from Solanum pennellii LA716
- 1.1k Downloads
The RXopJ4 resistance locus from the wild accession Solanum pennellii (Sp) LA716 confers resistance to bacterial spot disease of tomato (S. lycopersicum, Sl) caused by Xanthomonas perforans (Xp). RXopJ4 resistance depends on recognition of the pathogen type III effector protein XopJ4. We used a collection of Sp introgression lines (ILs) to narrow the RXopJ4 locus to a 4.2-Mb segment on the long arm of chromosome 6, encompassed by the ILs 6-2 and 6-2-2. We then adapted or developed a collection of 14 molecular markers to map on a segregating F2 population from a cross between the susceptible parent Sl FL8000 and the resistant parent RXopJ4 8000 OC7. In the F2 population, a 190-kb segment between the markers J350 and J352 cosegregated with resistance. This fine mapping will enable both the identification of candidate genes and the detection of resistant plants using cosegregating markers. The RXopJ4 resistance gene(s), in combination with other recently characterized genes and a quantitative trait locus (QTL) for bacterial spot disease resistance, will likely be an effective tool for the development of durable resistance in cultivated tomato.
We thank R. Bart and S. Goritschnig for critical reading of the manuscript. This research was supported by the Two Blades Foundation and a National Science Foundation Graduate Research Fellowship (Awarded to M.S.).
- Astua-Monge G, Minsavage GV, Stall RE, Vallejos CE, Davis MJ, Jones JB (2000) Xv4-vrxv4: a new gene-for-gene interaction identified between Xanthomonas campestris pv. vesicatoria race T3 and wild tomato relative Lycopersicon pennellii. Mol Plant Microbe Interact 13:1346–1355PubMedCrossRefGoogle Scholar
- Bart R, Cohn M, Kassen A, McCallum EJ, Shybut M, Petriello A, Krasileva K, Dahlbeck D, Medina C, Alicai T et al (2012) PNAS Plus: High-throughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance. Proc Natl Acad Sci USA. doi: 10.1073/pnas.1208003109 Google Scholar
- Glades Crop Care (1999) Crop profiles for south Florida tomatoes. http://www.gladescropcare.com/CP_tomatoes.pdf:1-24. Accessed 11 June 2012
- Jones JB, Lacy G, Bouzar H, Minsavage G, Stall R, Schaad N (2005) Bacterial spot: worldwide distribution, importance and review. Acta Hort 695:27–33Google Scholar
- Obradovic A, Jones JB, Balogh B, Momol MT (2008) Integrated management of tomato bacterial spot. In: Ciancio A, Mukerji KG (eds) Integrated management of diseases caused by fungi, phytoplasma and bacteria. Springer Science+Business Media, Dordrecht, pp 211–223Google Scholar
- Tai T, Dahlbeck D, Clark ET, Gajiwala P, Pasion R, Whalen MC, Stall RE, Staskawicz BJ (1999) Expression of the Bs2 pepper gene confers resistance to bacterial spot disease in tomato. Proc Natl Acad Sci USA 96:14153–14158Google Scholar