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Theoretical and Applied Genetics

, Volume 126, Issue 2, pp 523–533 | Cite as

Molecular mapping of Yr53, a new gene for stripe rust resistance in durum wheat accession PI 480148 and its transfer to common wheat

  • L. S. Xu
  • M. N. Wang
  • P. Cheng
  • Z. S. Kang
  • S. H. Hulbert
  • X. M. Chen
Original Paper

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most damaging diseases of wheat worldwide. It is essential to identify new genes for effective resistance against the disease. Durum wheat PI 480148, originally from Ethiopia, was resistant in all seedling tests with several predominant Pst races in the US under controlled greenhouse conditions and at multiple locations subject to natural infection for several years. To map the resistance gene(s) and to transfer it to common wheat, a cross was made between PI 480148 and susceptible common wheat genotype Avocet S (AvS). Resistant F3 plants with 42 chromosomes were selected cytologically and by testing with Pst race PST-100. A total of 157 F4 plants from a single F3 plant with 2n = 42 tested with PST-100 segregated in a 3 resistant: 1 susceptible ratio, indicating that a single dominant gene from PI 480148 conferred resistance. Using the F3:4 population and the resistance gene-analog polymorphism (RGAP) and simple sequence repeat (SSR) markers, the gene was mapped to the long arm of chromosome 2B. SSR marker Xwmc441 and RGAP marker XLRRrev/NLRRrev 350 flanked the resistance gene by 5.6 and 2.7 cM, respectively. The effective resistance of the gene to an Australian Pst isolate virulent to Yr5, which is also located on 2BL and confers resistance to all US Pst races, together with an allelism test of the two genes, indicated that the gene from PI 480148 is different from Yr5 and should be a new and useful gene for resistance to stripe rust. Resistant common wheat lines with plant types similar to AvS were selected for use in breeding programs.

Keywords

Simple Sequence Repeat Marker Durum Wheat Common Wheat Stripe Rust Wheat Genotype 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was supported by the US Department of Agriculture, Agricultural Research Service (Project No. 5348-22000-014-00D), Vogel Foundation (Project No. 13C-3061-3824) and Washington Wheat Commission (Project No. 13C-3061-3925). PPNS No. 0603, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center, Project Number WNP00663, Washington State University, Pullman, WA 99164-6430, USA. A scholarship from the China Scholarship Council to Liangsheng Xu is gratefully acknowledged. The research was also a part of the Northwest A&F University 111 Project supported by the Ministry of Education of China (Project No. B07049). We are grateful to Dr. Colin Wellings for providing the Australian stripe rust isolate. We thank Drs. Robert McIntosh and Michael Pumphrey for critical reviews of the manuscript.

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Copyright information

© Springer-Verlag Berlin Heidelberg (outside the USA) 2012

Authors and Affiliations

  • L. S. Xu
    • 1
    • 2
  • M. N. Wang
    • 2
  • P. Cheng
    • 2
  • Z. S. Kang
    • 1
  • S. H. Hulbert
    • 2
  • X. M. Chen
    • 2
    • 3
  1. 1.State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for AgricultureNorthwest A&F UniversityYanglingPeople’s Republic of China
  2. 2.Department of Plant PathologyWashington State UniversityPullmanUSA
  3. 3.Wheat Genetics, Quality, Physiology and Disease Research UnitUS Department of Agriculture-Agricultural Research Service (USDA-ARS)PullmanUSA

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