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Theoretical and Applied Genetics

, Volume 126, Issue 1, pp 265–274 | Cite as

Putative Thinopyrum intermedium-derived stripe rust resistance gene Yr50 maps on wheat chromosome arm 4BL

  • Jie Liu
  • Zhijian ChangEmail author
  • Xiaojun Zhang
  • Zujun Yang
  • Xin Li
  • Juqing Jia
  • Haixian Zhan
  • Huijuan Guo
  • Jianming Wang
Original Paper

Abstract

Stripe rust-resistant wheat introgression line CH223 was developed by crossing the resistant partial amphiploid TAI7047 derived from Thinopyrum intermedium with susceptible cultivars. The resistance is effective against all the existing Chinese stripe rust races, including the most widely virulent and predominant pathotypes CYR32 and CYR33. Cytological analyses using GISH detected no chromosomal segments from Th. intermedium. It was presumed that the segment was too small to be detected. Normal bivalent pairing at meiosis in CH223 and its hybrids confirmed its stability. Genetic analysis of the F1, F2, F3 and BC1 populations from crosses of CH223 with susceptible lines indicated that resistance was controlled by a single dominant gene. The resistance gene was mapped using an F2:3 population from Taichung 29/CH223. The gene was linked to five co-dominant genomic SSR markers, Xgwm540, Xbarc1096, Xwmc47, Xwmc310 and Xgpw7272, and flanked by Xbarc1096 and Xwmc47 at 8.0 and 7.2 cM, respectively. Using the Chinese Spring nulli-tetrasomic and ditelosomic lines, the polymorphic markers and the resistance gene were assigned to chromosome arm 4BL. As no permanently named stripe rust resistance genes had been assigned to chromosome 4BL, this new resistance gene is designated Yr50. The gene, together with the identified closely linked markers, could be used in marker-assisted selection to combine two or more resistance genes in a single genotype.

Keywords

Stripe Rust Chinese Spring Infection Type Stripe Rust Resistance Barley Yellow Dwarf Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors are grateful to Drs. Robert McIntosh and Peng Zhang (Plant Breeding Institute, University of Sydney) and Dr. Xianming Chen (Washington State University) for critical reviews of this manuscript, to Dr. Fangpu Han for technical guidance in GISH analysis and to Prof. Zhensheng Kang (Northwest A&F University, China) for seedling resistance testing. This study was funded by the National Natural Science Foundation (31171839 and 30671299), Shanxi Provincial Program of International S & T Cooperation (2012081006-2) and Research Project Supported by Shanxi Scholarship Council (2012-102) of China and the Opening Project of State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Science.

References

  1. Autrique E, Singh R, Tanksley S, Sorrells M (1995) Molecular markers for four leaf rust resistance genes introgressed into wheat from wild relatives. Genome 38:75–83PubMedCrossRefGoogle Scholar
  2. Bai Y, Li X, Zhang C, Zhang X, Zhan H, Chang Z (2011) Inheritance of stripe rust resistance gene in wheat line CH7103 introgressed from Thinopyrum in wheat and its allelism with known genes. J Triticeae Crops 31:364–369Google Scholar
  3. Bansal U, Forrest K, Hayden M, Miah H, Singh D, Bariana H (2011) Characterisation of a new stripe rust resistance gene Yr47 and its genetic association with the leaf rust resistance gene Lr52. Theor Appl Genet 122:1461–1466PubMedCrossRefGoogle Scholar
  4. Chang Z, Zhang X, Yang Z, Zhan H, Li X, Liu C, Zhang C (2010) Characterization of a partial wheat–Thinopyrum intermedium amphiploid and its reaction to fungal diseases of wheat. Hereditas 147:304–312PubMedCrossRefGoogle Scholar
  5. Chen X (2005) Epidemiology and control of stripe rust [Puccinia striiformis f. sp. tritici] on wheat. Can J Plant Pathol 27:314–337CrossRefGoogle Scholar
  6. Chen W, Wu L, Liu T, Xu S, Jin S, Peng Y, Wang B (2009) Race dynamics, diversity, and virulence evolution in Puccinia stiiformis f. sp. tritici, the causal agent of wheat stripe rust in China from 2003 to 2007. Plant Dis 93:1093–1101CrossRefGoogle Scholar
  7. Cheng P, Chen X (2010) Molecular mapping of a gene for stripe rust resistance in spring wheat cultivar IDO377s. Theor Appl Genet 121:195–204PubMedCrossRefGoogle Scholar
  8. Dong Y, Bu X, Luan Y, He M, Liu B (2004) Molecular characterization of a cryptic wheat–Thinopyrum intermedium translocation line: evidence for genomic instability in nascent allopolyploid and aneuploid lines. Genet Mol Biol 27:237–241CrossRefGoogle Scholar
  9. Fedak G (1999) Molecular aids for integration of alien chromatin through wide crosses. Genome 42:584–591CrossRefGoogle Scholar
  10. Fedak G, Han F (2005) Characterization of derivatives from wheat–Thinopyrum wide crosses. Cytogenet Genome Res 109:350–359CrossRefGoogle Scholar
  11. Friebe B, Jiang J, Raupp WJ, McIntosh RA, Gill BS (1996) Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87CrossRefGoogle Scholar
  12. Fu D, Uauy C, Distelfeld A, Blechl A, Epstein L, Chen X, Sela H, Fahima T, Dubcovsky J (2009) A kinase-START gene confers temperature-dependent resistance to wheat stripe rust. Science 323:1357–1360PubMedCrossRefGoogle Scholar
  13. Han F, Fedak G, Benabdelmouna A, Armstrong K, Ouellet T (2003) Characterization of six wheat × Thinopyrum intermedium derivatives by GISH, RFLP and multicolor GISH. Genome 46:490–495PubMedCrossRefGoogle Scholar
  14. Han F, Lamb JC, Birchler JA (2006) High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize. Proc Natl Acad Sci USA 103:3238–3243PubMedCrossRefGoogle Scholar
  15. He R, Chang Z, Yang Z, Yuan Z, Zhan H, Zhang X, Liu J (2009) Inheritance and mapping of powdery mildew resistance gene Pm43 introgressed from Thinopyrum intermedium into wheat. Theor Appl Genet 118:1173–1180PubMedCrossRefGoogle Scholar
  16. Herrera-Foessel SA, Lagudah ES, Huerta-Epino J, Hayden M, Bariana H, Singh D, Singh RP (2011) New slow-rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 in wheat are pleiotropic or closely linked. Theor Appl Genet 122:239–249PubMedCrossRefGoogle Scholar
  17. Hu L, Li G, Zeng Z, Chang Z, Liu C, Zhou J, Yang Z (2011) Molecular cytogenetic identification of a new wheat-Thinopyrum substitution line with stripe rust resistance. Euphytica 177:169–177CrossRefGoogle Scholar
  18. Jia J, Li G, Liu C, Lei M, Yang Z (2011) Characterization of wheat yellow rust resistance gene Yr17 using EST-SSR and rice syntenic region. Cereal Res Commun 39:88–99CrossRefGoogle Scholar
  19. Jiang J, Friebe B, Gill BS (1994) Recent advances in alien gene transfer in wheat. Euphytica 73:199–212CrossRefGoogle Scholar
  20. Kang Z, Zhao J, Han D, Zhang H, Wang X, Wang C, Han Q, Guo J, Huang L (2010) Status of wheat rust research and control in China. BGRI 2010, Technical Workshop, St Petersburg, 30–31 May 2010Google Scholar
  21. Kato A, Lamb JC, Birchler JA (2004) Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize. Proc Natl Acad Sci USA 101:13554–13559PubMedCrossRefGoogle Scholar
  22. Kuraparthy V, Sood S, Chhuneja P, Dhaliwal H, Kaur S, Bowden R, Gill BS (2007) A cryptic wheat–Aegilops triuncialis translocation with leaf rust resistance gene Lr58. Crop Sci 47:1995–2003CrossRefGoogle Scholar
  23. Li Z, Zeng S (2002) Wheat rust in China (In Chinese). China Agricultural Press, BeijingGoogle Scholar
  24. Li G, Li Z, Yang W, Zhang Y, He Z, Xu S, Singh R, Qu T, Xia X (2006a) Molecular mapping of stripe rust resistance gene YrCH42 in Chinese wheat cultivar Chuanmai 42 and its allelism with Yr24 and Yr26. Theor Appl Genet 112:1434–1440PubMedCrossRefGoogle Scholar
  25. Li Z, Xia X, Zhou X, Niu Y, He Z, Zhang Y, Li G, Wan A, Wang D, Chen X, Lu Q, Singh R (2006b) Seedling and slow rusting resistance to stripe rust in Chinese common wheats. Plant Dis 90:1302–1312CrossRefGoogle Scholar
  26. Li Q, Chen X, Wang M, Jing J (2011) Yr45, a new wheat gene for stripe rust resistance on the long arm of chromosome 3D. Theor Appl Genet 122:189–197PubMedCrossRefGoogle Scholar
  27. Lillemo M, Asalf B, Singh R, Huerta-Espino J, Chen X, He Z, Bjørnstad Å (2008) The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theor Appl Genet 116:1155–1166PubMedCrossRefGoogle Scholar
  28. Lincoln SE, Daly MJ, Lander ES (1993) Constructing linkage maps with MAPMAKER/Exp Version 3.0. A tutorial reference manual, 3rd edn. Whitehead Institute for Medical Res, CambridgeGoogle Scholar
  29. Liu TG, Peng YL, Zhang ZY (2010) First detection of virulence in Puccinia striiformis f. sp. tritici in China to resistance genes Yr24 (=Yr26) present in wheat cultivar Chuanmai 42. Plant Dis 94:1163CrossRefGoogle Scholar
  30. Lowe I, Jankuloski L, Chao S, Chen X, See D, Dubcovsky J (2011) Mapping and validation of Yr48 and other QTL conferring partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat. Theor Appl Genet 123:143–157PubMedCrossRefGoogle Scholar
  31. Luo P, Ren Z, Zhang H, Zhang H (2005) Identification, chromosome location, and diagnostic markers for a new gene (YrCN19) for resistance to wheat stripe rust. Phytopathology 95:1266–1270PubMedCrossRefGoogle Scholar
  32. Luo P, Luo H, Chang Z, Zhang H, Zhang M, Ren Z (2009) Characterization and chromosomal location of Pm40 in common wheat: a new gene for resistance to powdery mildew derived from Elytrigia intermedium. Theor Appl Genet 118:1059–1064PubMedCrossRefGoogle Scholar
  33. Marais F, Marais A, McCallum B, Pretorius Z (2009) Transfer of leaf rust and stripe rust resistance genes Lr62 and Yr42 from Aegilops neglecta Req. ex Bertol. to common wheat. Crop Sci 49:871–879CrossRefGoogle Scholar
  34. McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts, an atlas of resistance genes. Kluwer Academic Publishers, Dordrecht, p 174Google Scholar
  35. McIntosh R, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Somers D, Appels R, Devos K (2008) Catalogue of gene symbols for wheat. In: Proc 11th Int Wheat Genet Symp, University of Sydney Press, Australia. http://wheat.pw.usda.gov/GG2/Triticum/wgc/2008/
  36. McIntosh R, Dubcovsky J, Rogers J, Morris C, Appels R, Xia X (2011) Catalogue of gene symbols for wheat: 2011 supplement. Annu Wheat Newslett 56:273–282Google Scholar
  37. Murphy L, Santra D, Kidwell K, Yan G, Chen X, Campbell K (2009) Linkage maps of wheat stripe rust resistance genes Yr5 and Yr15 for use in marker-assisted selection. Crop Sci 49:1786–1790CrossRefGoogle Scholar
  38. Paillard S, Schnurbusch T, Winzeler M, Messmer M, Sourdille P, Abderhalden O, Keller B, Schachermayr G (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.). Theor Appl Genet 107:1235–1242PubMedCrossRefGoogle Scholar
  39. Plaschke J, Börner A, Wendehake K, Ganal MW, Röder MS (1996) The use of aneuploids for the chromosomal assignment of microsatellite loci. Euphytica 89:33–40CrossRefGoogle Scholar
  40. Qi L, Friebe B, Zhang P, Gill BS (2007) Homoeologous recombination, chromosome engineering and crop improvement. Chrom Res 15:3–19PubMedCrossRefGoogle Scholar
  41. Somers DJ, Isaac P, Edwards K (2004) A high density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114PubMedCrossRefGoogle Scholar
  42. Stein N, Herren G, Keller B (2001) A new DNA extraction method for high-throughout marker analysis in a large-genome species such as Triticum aestivum. Plant Breed 120:354–356CrossRefGoogle Scholar
  43. Sun Q, Wei Y, Ni C, Xie C, Yang T (2002) Microsatellite marker for yellow rust resistance gene Yr5 introgressed from spelt wheat. Plant Breed 121:539–541CrossRefGoogle Scholar
  44. Uauy C, Brevis J, Chen X, Khan I, Jackson L, Chicaiza O, Distenfeld A, Fahima T, Dubcovsky J (2005) High-temperature adult-plant stripe rust resistance gene Yr36 from Triticum turgidum ssp. dicoccoides is closely linked to the grain protein content locus Gpc-B1. Theor Appl Genet 112:97–105PubMedCrossRefGoogle Scholar
  45. Wan A, Zhao Z, Chen X, He Z, Jin S, Jia Q, Yao G, Yang J, Wang B, Li G, Bi Y, Yuan Z (2004) Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici in China in 2002. Plant Dis 88:896–904CrossRefGoogle Scholar
  46. Wan A, Chen X, He Z (2007) Wheat stripe rust in China. Aust J Agri Res 58:605–619CrossRefGoogle Scholar
  47. Wang K, Xie S, Liu X, Wu L, Wang J, Chen Y (1988) Progress in studies on wheat stripe rust in China. (In Chinese with English abstr) Sci Agric Sin 16:80–85Google Scholar
  48. Yan J, Chang Z, Sun M, Zhang X, Zhan H, Li X (2010) Inheritance of wheat stripe rust resistance of alien introgression CH223 from Thinopyrum intermedium and its cytological characterization. Acta Phytophylacica Sinica 37:419–424Google Scholar
  49. Yang Z, Xie C, Sun Q (2003) Situation of the sources of stripe rust resistance of wheat in the post-CY32 era in China. Acta Agron Sin 29:161–168Google Scholar
  50. Yang Z, Li G, Chang Z, Zhou J, Ren Z (2006) Characterization of a partial amphiploid between Triticum aestivum cv. Chinese Spring and Thinopyrum intermedium ssp. Trichophorum. Euphytica 149:11–17CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Jie Liu
    • 1
  • Zhijian Chang
    • 2
    • 3
    Email author
  • Xiaojun Zhang
    • 2
    • 3
  • Zujun Yang
    • 4
  • Xin Li
    • 2
    • 3
  • Juqing Jia
    • 5
  • Haixian Zhan
    • 2
    • 3
  • Huijuan Guo
    • 2
    • 3
  • Jianming Wang
    • 5
  1. 1.College of Life ScienceShanxi UniversityTaiyuanChina
  2. 2.Institute of Crop ScienceShanxi Academy of Agricultural SciencesTaiyuanChina
  3. 3.Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess PlateauMinistry of AgricultureTaiyuanChina
  4. 4.School of Life Science and TechnologyUniversity of Electronic Science and Technology of ChinaChengduChina
  5. 5.College of AgronomyShanxi Agricultural UniversityTaiguChina

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