Advertisement

Theoretical and Applied Genetics

, Volume 125, Issue 7, pp 1403–1411 | Cite as

Inheritance and molecular mapping of a gene conferring seedling resistance against Puccinia hordei in the barley cultivar Ricardo

  • K. S. Sandhu
  • K. L. Forrest
  • S. Kong
  • U. K. Bansal
  • D. Singh
  • M. J. Hayden
  • R. F. ParkEmail author
Original Paper

Abstract

Genetic studies were undertaken to determine the inheritance and genomic location of uncharacterised seedling resistance to leaf rust, caused by Puccinia hordei, in the barley cultivar Ricardo. The resistance was shown to be conferred by a single dominant gene, which was tentatively designated RphRic. Bulk segregant analysis (BSA) and genetic mapping of an F3 mapping population using multiplex-ready SSR genotyping and Illumina GoldenGate SNP assay located RphRic in chromosome 4H. Given that this is the first gene for leaf rust resistance mapped on chromosome 4H, it was designated Rph21. The presence of an additional gene, Rph2, in Ricardo, was confirmed by the test of allelism. The seedling gene Rph21 has shown effectiveness against all Australian pathotypes of P. hordei tested since at least 1992 and hence represents a new and useful source of resistance to this pathogen.

Keywords

Leaf Rust Rust Resistance Rust Resistance Gene Adult Plant Resistance Bulk Segregant Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

K. S. Sandhu would like to sincerely thank and acknowledge the Australian Grains Research and Development Corporation for the provision of a Postgraduate Research Scholarship and the support provided by the University of Sydney that enabled these studies to be initiated and completed. Valuable technical support provided by Mr Matthew Williams is also acknowledged.

References

  1. Arnst BJ, Martens JW, Wright GM, Burnett PA, Sanderson FR (1979) Incidence, importance and virulence of Puccinia hordei on barley in New Zealand. Ann Appl Biol 92:185–190CrossRefGoogle Scholar
  2. Bansal UK, Hayden MJ, Gill EMB, Bariana HS (2010) Chromosomal location of an uncharacterised stripe rust resistance gene in wheat. Euphytica 171:121–127CrossRefGoogle Scholar
  3. Borovkova IG, Jin Y, Steffenson BJ, Kilian A, Blake TK, Kleinhofs A (1997) Identification and mapping of a leaf rust resistance gene in barley line Q21861. Genome 40:236–241PubMedCrossRefGoogle Scholar
  4. Borovkova IG, Jin Y, Steffenson BJ (1998) Chromosomal location and genetic relationship of leaf rust resistance genes Rph9 and Rph12 in barley. Phytopathol 88:76–80CrossRefGoogle Scholar
  5. Brunner S, Keller B, Feuillet C (2000) Molecular mapping of the Rph7.g leaf rust resistance gene in barley (Hordeum vulgare L.). Theor Appl Genet 101:783–788CrossRefGoogle Scholar
  6. Clifford BC (1985) Barley leaf rust. In: Roelfs AP, Bushnell WR (eds) The cereal rusts. Academic, Orlando, pp 173–205Google Scholar
  7. Close TJ, Bhat PR, Lonardi S et al (2009) Development and implementation of high–throughput SNP genotyping in barley. BMC Genomics 10:582PubMedCrossRefGoogle Scholar
  8. Cotterill PJ, Rees RG, Platz GJ, Dill-Macky R (1992) Effects of leaf rust on selected Australian barleys. Aust J Exp Agric 32:747–751CrossRefGoogle Scholar
  9. Fan JB, Gunderson KL, Bibikova M, Yeakley JM, Chen J, Wickham Garcia E, Lebruska LL, Laurent M, Shen R, Barker D (2006) Illumina universal bead arrays. Methods Enzymol 410:57–73PubMedCrossRefGoogle Scholar
  10. Franckowiak JD, Jin Y, Steffenson BJ (1997) Recommended allele symbols for leaf rust resistance genes in barley. Barley Genet Newslett 27:36–44Google Scholar
  11. Ganal MW, Altmann T, Röder MS (2009) SNP identification in crop plants. Curr Opin Plant Biol 12:211–217PubMedCrossRefGoogle Scholar
  12. Golegaonkar PG (2007) Genetic and molecular analyses of resistance to rust diseases in barley. PhD thesis, The University of Sydney, pp 58Google Scholar
  13. Golegaonkar PG, Singh D, Park RF (2009) Evaluation of seedling and adult plant resistance to Puccinia hordei in barley. Euphytica 166:183–197CrossRefGoogle Scholar
  14. Gousseau HDM, Deverall BJ, Mcintosh RA (1985) Temperature-sensitivity of the expression of resistance to Puccinia graminis conferred by the Sr15, Sr9b and Sr14 genes in wheat. Physiol Plant Path 27:335–343CrossRefGoogle Scholar
  15. Graner A, Streng S, Drescher A, Jin Y, Borovkova I, Steffenson BJ (2000) Molecular mapping of the leaf rust resistance gene Rph7 in barley. Plant Breed 119:389–392CrossRefGoogle Scholar
  16. Griffey CA, Das MK, Baldwin RE, Waldenmaier CM (1994) Yield losses in winter barley resulting from a new race of Puccinia hordei in North America. Plant Dis 78:256–260CrossRefGoogle Scholar
  17. Gupta PK, Varshney RK (2000) The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica 113:163–185CrossRefGoogle Scholar
  18. Hayden MJ, Nguyen TM, Waterman A, Chalmers KJ (2008) Multiplex-Ready PCR: a new method for multiplexed SSR and SNP genotyping. BMC Genomics 9:80. doi: 10.1186/1471-2164-9-80 PubMedCrossRefGoogle Scholar
  19. Henderson MT (1945) Studies of sources of resistance and inheritance of reaction to leaf rust Puccinia anomala Rostr. in barley. PhD thesis, University of MinnesotaGoogle Scholar
  20. Hickey LH, Lawson W, Platz GJ, Dieters M, Arief VN, Germán S, Fletcher S, Park RF, Singh D, Pereyra S, Franckowiak J (2011) Mapping Rph20: a gene conferring adult plant resistance to Puccinia hordei in barley. Theor Appl Genet 123:55–68PubMedCrossRefGoogle Scholar
  21. König J, Kopahnke D, Steffenson BJ, Przulj N, Romeis T, Röder MS, Ordon F, Perovic D (2012) Genetic mapping of a leaf rust resistance gene in the former Yugoslavian barley landrace MBR1012. Mol Breed. doi: 10.1007/s11032-012-9712-0 Google Scholar
  22. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175Google Scholar
  23. Langridge P, Barr AR (2003) Better barley faster: the role of marker assisted selection-preface. Aust J Agric Res 54:1–5CrossRefGoogle Scholar
  24. Levine MN, Cherewick WJ (1952) Studies on dwarf leaf rust of barley. USDA Tech Bull 1056:1–17Google Scholar
  25. Mammadov JA, Zwonitzer JC, Biyashev RM, Griffey CA, Jin Y, Steffenson BJ, Maroof MAS (2003) Molecular mapping of leaf rust resistance gene Rph5 in barley. Crop Sci 43:388–393CrossRefGoogle Scholar
  26. Manly KF, Cudmore RHJ, Meer JM (2001) Map manager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932PubMedCrossRefGoogle Scholar
  27. Mayer KFX, Martis M, Hedley PE et al (2011) Unlocking the barley genome by chromosomal and comparative genomics. Plant Cell 23:1249–1263PubMedCrossRefGoogle Scholar
  28. Melville SC, Griffin GW, Jemmett JL (1976) Effects of fungicide spraying on brown rust and yield in spring barley. Plant Pathol 25:99–107CrossRefGoogle Scholar
  29. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832PubMedCrossRefGoogle Scholar
  30. Moseman JG, Roan CW (1959) Physiologic races of barley leaf rust (Puccinia hordei) isolated in the United States from 1956 through 1958. Plant Dis 43:1000–1003Google Scholar
  31. Muñoz-Amatriaín M, Moscou MJ, Bhat PR et al (2011) An improved consensus linkage map of barley based on flow-sorted chromosomes and single nucleotide polymorphism markers. Plant Genome 4:238–249CrossRefGoogle Scholar
  32. Murray GM, Brennan JP (2010) Estimating disease losses to the Australian barley industry. Aust Plant Pathol 39:85–96CrossRefGoogle Scholar
  33. Park RF (2003) Pathogenic specialization and pathotype distribution of Puccinia hordei in Australia, 1992 to 2001. Plant Dis 87:1311–1316CrossRefGoogle Scholar
  34. Park RF (2010) Annual report: cereal rust survey 2009–2010. The University of Sydney, NSW, pp 1–12Google Scholar
  35. Park RF, Karakousis A (2002) Characterization and mapping of gene Rph19 conferring resistance to Puccinia hordei in the cultivar ‘Reka 1’ and several Australian barleys. Plant Breed 121:232–236CrossRefGoogle Scholar
  36. Perovic D, Stein N, Zhang H, Drescher A, Prasad M, Kota R, Kopahnke D, Graner A (2004) An integrated approach for comparative mapping in rice and barley with special reference to the Rph16 resistance locus. Funct Integr Genomics 4:74–83. doi: 10.1007/s10142-003-0100-z PubMedCrossRefGoogle Scholar
  37. Qamar M, Ahamad S, Shah A, Wellings C, Batool F (2008) Postulation of stripe rust resistant genes in some Australian bread wheat cultivars and their response to temperature. Pak J Bot 40:2573–2585Google Scholar
  38. Ramsay L, Macaulay M, Ivanissevich DS, MacLean K, Cardle L, Fuller J, Edwards KJ, Tuvesson S, Morgante M, Masssari A, Maestri E, Marmiroli N, Sjakste T, Ganal M, Powell W, Waugh R (2000) A simple sequence repeat-based linkage map of barley. Genetics 156:1997–2005PubMedGoogle Scholar
  39. Roane CW, Starling TM (1967) Inheritance of reaction to Puccinia hordei in barley. II. Gene symbols for loci in differential cultivars. Phytopathology 57:66–68Google Scholar
  40. Singh B, Bansal UK, Forrest KL, Hayden MJ, Hare RA, Bariana HS (2010) Inheritance and chromosome location of leaf rust resistance in durum wheat cultivar Wollaroi. Euphytica 175:351–355CrossRefGoogle Scholar
  41. Starling TM (1956) Sources, inheritance, and linkage relationships of resistance to race 4 of leaf rust (Puccinia hordei Otth.) race 9 of powdery mildew (Erysiphe graminia hordei El. Marchal.), and certain agronomic characters in barley. J Sci 30:438–439Google Scholar
  42. Steffenson BJ, Jin Y (1997) A multi-allelic series at the Rph2 locus for leaf rust resistance in barley. Cereal Rusts PM Bull 24:74–75Google Scholar
  43. Stöcker GG (1983) Development of a differential set for the race analysis of Puccinia hordei Otth. J Phytopathol 107:309–317CrossRefGoogle Scholar
  44. Sun Y, Steffenson B (1997) Effect of incubation time and temperature on the phenotypic expression of rpg4 to Puccunia graminis f. sp. tritici in barley. Can J Plant Pathol 19:25–29CrossRefGoogle Scholar
  45. Tan BH (1977) Evaluating host differentials of Puccinia hordei. Cereal Rusts Bull 5:17–23Google Scholar
  46. Ulrich SE (2011) Barley: production, improvement and uses. Blackwell, OxfordGoogle Scholar
  47. Varshney RK, Marcel TA, Ramsay L, Russel J, Roder MS, Stein N, Waugh R, Langridge P, Niks RE, Graner A (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theor Appl Genet 114:1091–1103PubMedCrossRefGoogle Scholar
  48. Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Heredity 93:77–78CrossRefGoogle Scholar
  49. Wallwork H, Preece P, Cotterill PJ (1992) Puccinia hordei on barley and Ornithogalum umbellatum in South Australia. Aust Plant Pathol 21:95–97CrossRefGoogle Scholar
  50. Weerasena JS, Steffenson BJ, Falk AB (2004) Conversion of an amplified fragment length polymorphism marker into a co-dominant marker in the mapping of the Rph15 gene conferring resistance to barley leaf rust, Puccinia hordei Otth. Theor Appl Genet 108:712–719PubMedCrossRefGoogle Scholar
  51. Yahyaoui AH, Sharp EL, Reinhold M (1988) New sources of resistance to Puccinia hordei in barley land race cultivars. Phytopathology 78:905–908CrossRefGoogle Scholar
  52. Zhong SB, Effertz RJ, Jin Y, Franckowiak JD, Steffenson BJ (2003) Molecular mapping of the leaf rust resistance gene Rph6 in barley and its linkage relationships with Rph5 and Rph7. Phytopathology 93:604–609PubMedCrossRefGoogle Scholar
  53. Zloten RR (1952) Inheritance of reaction of leaf rust in barley. MSc thesis, University of ManitobaGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • K. S. Sandhu
    • 1
    • 2
  • K. L. Forrest
    • 3
  • S. Kong
    • 3
  • U. K. Bansal
    • 1
  • D. Singh
    • 1
  • M. J. Hayden
    • 3
  • R. F. Park
    • 1
    Email author
  1. 1.Plant Breeding InstituteThe University of SydneyNarellanAustralia
  2. 2.Department of Agriculture, Fisheries and Forestry, Crop and Food Science, Agri-Science QueenslandLeslie Research CentreToowoombaAustralia
  3. 3.Department of Primary IndustriesVictorian AgriBioscience CentreBundooraAustralia

Personalised recommendations