Theoretical and Applied Genetics

, Volume 125, Issue 5, pp 1057–1068 | Cite as

SNP identification and allelic-specific PCR markers development for TaGW2, a gene linked to wheat kernel weight

  • Zibo Yang
  • Zhiyuan Bai
  • Xiaolin Li
  • Pei Wang
  • Qingxia Wu
  • Lin Yang
  • Liqun Li
  • Xuejun Li
Original Paper

Abstract

TaGW2, an orthologous gene of rice OsGW2, has been associated with kernel width and weight of bread wheat (Triticum aestivum). Difference in TaGW2 coding sequence was not found among different wheat varieties in previous researches. In this study, we found eight exons and seven introns in TaGW2 with a full-length cDNA sequence of 1,275 bp, which contains a conserved function domain and seven splice sites that shared homology with rice OsGW2. A single T-base insertion in the eighth exon of TaGW2 on chromosome 6A was detected in a large-kernel wheat variety, Lankaodali. This insertion mutation reduces the coding protein sequence from normal 424 amino acids (~47.2 kDa) to 328 amino acids (~37.1 kDa) by truncating 96 amino acids. The result was validated by identifying histidine-tagged TaGW2 proteins encoded by both alleles of the mutant and the wild types in SDS-PAGE. Allele-specific PCR markers were developed based on the single nucleotide polymorphism (SNP) site. The SNP markers were genotyped for an F2 segregation population from the cross of Lankaodali × Chinese Spring. Seed traits of F2:3 families were evaluated in three different environments. The association analysis indicated that F2:3 families with the mutated TaGW2 allele significantly increased kernel width (KW) and thousand-kernel weight (TKW), and slightly improved kernel length (KL). Using the SNP markers, another two varieties harbored the mutated TaGW2 allele were successfully identified from 22 additional wheat varieties, and they both have large KW and TKW. Cloning and sequencing of the gene further confirmed the functions of the mutated allele of TaGW2 in the two large kernel varieties. The results suggested that TaGW2 may negatively regulate kernel size variation, which shares the same function as OsGW2 in rice. The successful development of SNP markers provides a useful tool for improving kernel yield in wheat.

Supplementary material

122_2012_1895_MOESM1_ESM.doc (5.6 mb)
Supplementary material 1 (DOC 5756 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Zibo Yang
    • 1
  • Zhiyuan Bai
    • 1
  • Xiaolin Li
    • 1
  • Pei Wang
    • 1
  • Qingxia Wu
    • 1
  • Lin Yang
    • 1
  • Liqun Li
    • 1
  • Xuejun Li
    • 1
  1. 1.State Key Laboratory of Crop Stress Biology in Arid Areas and College of AgronomyNorthwest A&F UniversityYanglingPeople’s Republic of China

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