Theoretical and Applied Genetics

, Volume 125, Issue 4, pp 781–791 | Cite as

Mapping quantitative trait loci conferring resistance to rice black-streaked virus in maize (Zea mays L.)

  • Junwen Luan
  • Fei Wang
  • Yujie Li
  • Bin Zhang
  • Juren ZhangEmail author
Original Paper


Maize rough dwarf disease (MRDD) is one of the most serious virus diseases of maize worldwide, and it causes great reduction of maize production. In China, the pathogen was shown to be rice black-streaked virus (RBSDV). Currently, MRDD has spread broadly and leads to significant loss in China. However, there has been little research devoted to this disease. Our aims were to identify the markers and loci underlying resistance to this virus disease. In this study, segregation populations were constructed from two maize elite lines ‘90110’, which is highly resistant to MRDD and ‘Ye478’, which is highly susceptible to MRDD. The F2 and BC1 populations were used for bulk sergeant analysis (BSA) to identify resistance-related markers. One hundred and twenty F7:9 RILs were used for quantitative trait loci (QTL) mapping through the experiment of multiple environments over 3 years. Natural occurrence and artificial inoculation were both used and combined to determine the phenotype of plants. Five QTL, qMRD2, qMRD6, qMRD7, qMRD8 and qMRD10 were measured in the experiments. The qMRD8 on chromosome 8 was proved to be one major QTL conferring resistance to RBSDV disease in almost all traits and environments, which explained 12.0–28.9 % of the phenotypic variance for disease severity in this present study.


Quantitative Trait Locus Quantitative Trait Locus Mapping RILs Population Resistance Quantitative Trait Locus Disease Severity Index 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the National Basic Research Program of China (973 Program 2009CB118400) and National High Technology Research and Development Program of China (863 Program) (No. 2012AA10A306). We thank Professor Deng-hai Li (Laizhou Academy of Agricultural Sciences) for donating maize inbred line ‘Ye478’ and supporting to field tests. We thank Professor Cheng-he Zhang (Hebei Academy of Agricultural Sciences) for donating maize inbred line ‘90110’. We are grateful to Doctor Roberta Greenwood for help with editing the English of the paper.


  1. Bai FW, Yan J, Qu ZC, Zhang HW, Xu J, Ye MM, Shen DL (2002) Phylogenetic analysis reveals that a dwarfing disease on different cereal crops in China is due to rice black-streaked dwarf virus (RBSDV). Virus Genes 25:201–206PubMedCrossRefGoogle Scholar
  2. Bar-Tsur A, Saadi H, Antignus Y (1998) Resistance of corn genotypes to maize rough dwarf virus. Maydica 33:189–200Google Scholar
  3. Bassam BJ, Anollés GC, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80–83PubMedCrossRefGoogle Scholar
  4. Boccardo G, Milne RG (1984) Plant reovirus group. CMI/AAB Descriptions of plant viruses, no. 294. Commonwealth Mycology Institute and Association of Applied Biology, Kew, United KingdomGoogle Scholar
  5. Bonamico NC, Di Renzo MA, Ibañez MA, Borghi ML, Díaz DG, Salerno JC, Balzarini MG (2012) QTL analysis of resistance to Mal de Río Cuarto disease in maize using recombinant inbred lines. J Agric Sci. doi: 10.1017/S0021859611000943 Google Scholar
  6. Chaguè V, Mercier JC, Guènard M, Courcel A, Vedel F (1997) Identification of RAPD markers linked to a locus involved in quantitative resistance to TYLCV in tomato by bulked segregant analysis. Theor Appl Genet 95:671–677CrossRefGoogle Scholar
  7. Chen SX, Zhang QY (2005) Advance in research on rice black-streaked dwarf disease and maize rough dwarf disease in China. J Plant Prot 32:97–103Google Scholar
  8. Chen CX, Wang ZL, Yang DE, Ye CJ, Zhao YB, Jin DM, Weng ML, Wang B (2004) Molecular tagging and genetic mapping of the disease resistance gene RppQ to southern corn rust. Theor Appl Genet 108:945–950PubMedCrossRefGoogle Scholar
  9. Chen YP, Meng QC, Yuan JH (2008) Identification of the molecular markers linked to the MRDV-resistance locus in maize using SSR-BSA technique. J Jiangsu Agric Sci 24(5):590–594 (in Chinese with English abstract)Google Scholar
  10. Delano J (1999) A simple and reliable protocol for the detection of apple stem grooving virus by RT-PCR and in a multiplex PCR assay. J Virol Methods 83:1–9CrossRefGoogle Scholar
  11. Di Renzo MA, Bonamico NC, Díaz DD, Salerno JC, Ibañez MA, Gesumaria   (2002) Inheritance of resistance to Mal de Río Cuarto (MRC) disease in Zea mays L. J Agric Sci 139:47–53Google Scholar
  12. Di Renzo MA, Bonamico NC, Díaz DD, Ibañez MA, Faricell ME, Balzarini MGAND, Salerno JC (2004) Microsatellite markers linked to QTL for resistance to Mal de Río Cuarto disease in Zea mays L. J Agric Sci 142:289–295CrossRefGoogle Scholar
  13. Dintinger J, Verger D, Caiveau S, Risterucci AM, Gilles J, Chiroleu F, Courtois B, Reynaud B, Hamon P (2005) Genetic mapping of maize stripe disease resistance from the Mascarene source. Theor Appl Genet 111:347–359PubMedCrossRefGoogle Scholar
  14. Dovas CI, Eythymious K, Katis NI (2004) First report of maize rough dwarf virus (MRDV) on maize crops in Greece. Plant Pathol 53(2):238CrossRefGoogle Scholar
  15. Fang S, Yu J, Feng J, Han C, Li D, Liu Y (2001) Identification of rice black-streaked dwarf fijivirus in maize with rough dwarf disease in China. Arch Virol 146:167–170PubMedCrossRefGoogle Scholar
  16. Farinhó M, Coelho P, Carlier J, Svetleva D, Monteiro A, Leitão J (2004) Mapping of a locus for adult plant resistance to downy mildew in broccoli (Brassica oleracea convar. italica). Theor Appl Genet 109(7):1392–1398PubMedCrossRefGoogle Scholar
  17. Grau CR, Radke VL, Gillespie FL (1982) Resistance of soybean cultivars to Sclerotinia sclerotiorum. Plant Dis 66:506–508CrossRefGoogle Scholar
  18. Hallauer AR, Miranda JB (1988) Quantitative genetics in maize breeding, 2nd edn. Iowa State University Press, AmesGoogle Scholar
  19. Harpaz I, Minz G, Nitzani F (1958) Dwarf disease of maize. FAO Plant Protect Bull 7:43Google Scholar
  20. Hu KM, Qiu DY, Shen XL, Li XH, Wang SP (2008) Isolation and manipulation of quantitative trait loci for disease resistance in rice using a candidate gene approach. Mol Plant 1(5):786–793PubMedCrossRefGoogle Scholar
  21. Isogai M, Uyeda I, Choi JK (2001) Molecular diagnosis of rice black-streaked dwarf virus in Japan and Korea. J Plant Pathol 17(3):164–168Google Scholar
  22. Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175CrossRefGoogle Scholar
  23. Kreff ED, Pacheco MG, Díaz DG, Robredo CG, Puecher D, Céliz A, Salerno JC (2006) Resistance to Mal de Río Cuarto Virus in maize: a QTL analysis. J Basic Appl Genet 17:41–50Google Scholar
  24. Lehmensiek A, Esterhuizen AM, Van Staden D, Nelson SW, Retief AE (2001) Genetic mapping of gray leaf spot (GLS) resistance genes in maize. Theor Appl Genet 103:797–803CrossRefGoogle Scholar
  25. Li H, Ye G, Wang J (2007) A modified algorithm for the improvement of composite interval mapping. Genetics 175:361–374PubMedCrossRefGoogle Scholar
  26. Li H, Li Z, Wang J (2008) Inclusive composite interval mapping (ICIM) for digenic epistasis of quantitative traits in biparental populations. Theor Appl Genet 116:243–260PubMedCrossRefGoogle Scholar
  27. Lincoln S, Daly M, Lander E (1992) Constructing genetics maps with MAPMAKER/EXP 3.0. Whitehead Institute Technical Report, Whitehead Institute Cambridge, USAGoogle Scholar
  28. Liu ZZ, Chi SM, Song ZQ, Chen JT, Meng YJ (1996) Identification and analysis for the resistance of maize inbred lines and hybrids. J Maize Sci 4:68–70 (in Chinese with English abstract)Google Scholar
  29. Liu F, Niu Y, Deng H, Tan G (2007) Mapping of a major stripe rust resistance gene in Chinese native wheat variety Chike using microsatellite markers. J Genet Genomics 34(12):1123–1130PubMedCrossRefGoogle Scholar
  30. Louie R, Abt JJ (2004) Mechanical transmission of maize rough dwarf virus. Maydica 49:231–240Google Scholar
  31. Lu GY, Zhang LY, Tian ZL, Di DP, Miao HG (2009) Effect of maize resistance to rice black-streaked dwarf on tropism and survival of Laodelphax striatellus. J Hebei Agric Univ 32(5):73–76 (in Chinese with English abstract)Google Scholar
  32. Lübberstedt T, Ingvardsen C, Melchinger AE, Xing Y, Salomon R, Redinbaugh MG (2006) Two chromosome segments confer multiple potyvirus resistance in maize. Plant Breed 125:352–356CrossRefGoogle Scholar
  33. Maguire TL, Collins GG, Sedgley M (1994) A modified CTAB DNA extraction procedure for plants belonging to the family proteaceae. Plant Mol Biol Rep 12:106–109CrossRefGoogle Scholar
  34. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci 88(21):9828–9832PubMedCrossRefGoogle Scholar
  35. Ornaghi J, Boito G, Sanchez G, March G, Beviacqua J (1993) Studies on the populations of Delphacodes kuscheli Fennah in different years and agricultural areas. J Genet Breed 47:277–282Google Scholar
  36. Quarrie SA, Lazic-Lancic V, Kovacevic D, Steed A, Pekic S (1999) Bulk segregant analysis with molecular markers and its use for improving drought resistance in maize. J Exp Bot 50:1299–1306Google Scholar
  37. Redinbaugh MG, Pratt RC (2009) Virus resistance. In: Bennetzen J, Hake S (eds) Handbook of Maize: Its biology. Springer, New York, pp 251–270CrossRefGoogle Scholar
  38. Redinbaugh MG, Jones MW, Gingery RE (2004) The genetics of virus resistance in maize (Zea mays L.). Maydica 49:183–190Google Scholar
  39. Ruan YL, Jing DH, Xu RY, Jiang WL, Jin DD (1984) Research on rice black-streaked dwarf disease. J Zhejiang Agric Sci 4:185–192 (In Chinese with English abstract)Google Scholar
  40. Shikata E, Kitagawa Y (1977) Rice black-streaked dwarf virus: Its properties, morphology and intracellular localization. Virology 77(2):826–842PubMedCrossRefGoogle Scholar
  41. Tullu A, Buchwaldt L, Warkentin T, Taran B, Vandenberg A (2003) Genetics of resistance to anthracnose and identification of AFLP and RAPD markers linked to the resistance gene in PI 320937 germplasm of lentil (Lens culinaris Medikus). Theor Appl Genet 106(3):428–434PubMedGoogle Scholar
  42. Wang AL, Wang JJ, Chen ZH et al (1998) Identifying Method of Greyfly Spreading Virus under the Control of Net Shed. J Shanxi Agric Sci 26(2):68–69 (in Chinese with English abstract)Google Scholar
  43. Wang AL, Zhao DF, Chen ZH, Wang JJ, Shao XS, Wei GY (2000) Studies on genetic basis and recurrent selection effect of inbred line maize resistance to MRDV. J Maize Sci 8:80–82 (in Chinese with English abstract)Google Scholar
  44. Wang F, Qin F, Sui ZH et al (2006) Improved method for assaying maize plant resistance to maize rough dwarf disease by artificial inoculation and real-time RT-PCR. Eur J Plant Pathol 116:289–300CrossRefGoogle Scholar
  45. Wang F, Zhang YS, Zhuang YL, Qin GZ, Zhang JR (2007) Molecular mapping of three loci conferring resistance to maize (Zea mays L.) rough dwarf disease. Mol Plant Breed 5:178–179Google Scholar
  46. William HM, Hoisington D, Singh RP, González-de-León D (1997) Detection of quantitative trait loci associated with leaf rust resistance in bread wheat. Genome 40:253–260PubMedCrossRefGoogle Scholar
  47. Xu ML, Melchinger AE, Xia XC, Lübberstedt T (1999) High-resolution mapping of loci conferring resistance to sugarcane mosaic virus in maize using RFLP, SSR, and AFLP markers. Mol Gen Genet 261:574–581PubMedCrossRefGoogle Scholar
  48. Yang DE, Zhang CL, Zhang DS, Jin DM, Weng ML, Chen SJ, Nguyen H, Wang B (2004) Genetic analysis and molecular mapping of maize (Zea mays L.) stalk rot resistant gene Rfg1. Theor Appl Genet 108:706–711PubMedCrossRefGoogle Scholar
  49. Zhou T, Wu LJ et al (2011) Transmission of rice black-steaked dwarf virus from frozen infected leaves to healthy rice plants by small brown planthopper (Laodelphax striatellus). J Rice Sci 18(2):152–156CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Junwen Luan
    • 1
  • Fei Wang
    • 1
    • 2
  • Yujie Li
    • 1
  • Bin Zhang
    • 1
  • Juren Zhang
    • 1
    Email author
  1. 1.School of Life SciencesShandong UniversityJinanChina
  2. 2.School of Life SciencesShanghai UniversityShanghaiChina

Personalised recommendations