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Theoretical and Applied Genetics

, Volume 125, Issue 4, pp 781–791 | Cite as

Mapping quantitative trait loci conferring resistance to rice black-streaked virus in maize (Zea mays L.)

  • Junwen Luan
  • Fei Wang
  • Yujie Li
  • Bin Zhang
  • Juren ZhangEmail author
Original Paper

Abstract

Maize rough dwarf disease (MRDD) is one of the most serious virus diseases of maize worldwide, and it causes great reduction of maize production. In China, the pathogen was shown to be rice black-streaked virus (RBSDV). Currently, MRDD has spread broadly and leads to significant loss in China. However, there has been little research devoted to this disease. Our aims were to identify the markers and loci underlying resistance to this virus disease. In this study, segregation populations were constructed from two maize elite lines ‘90110’, which is highly resistant to MRDD and ‘Ye478’, which is highly susceptible to MRDD. The F2 and BC1 populations were used for bulk sergeant analysis (BSA) to identify resistance-related markers. One hundred and twenty F7:9 RILs were used for quantitative trait loci (QTL) mapping through the experiment of multiple environments over 3 years. Natural occurrence and artificial inoculation were both used and combined to determine the phenotype of plants. Five QTL, qMRD2, qMRD6, qMRD7, qMRD8 and qMRD10 were measured in the experiments. The qMRD8 on chromosome 8 was proved to be one major QTL conferring resistance to RBSDV disease in almost all traits and environments, which explained 12.0–28.9 % of the phenotypic variance for disease severity in this present study.

Keywords

Quantitative Trait Locus Quantitative Trait Locus Mapping RILs Population Resistance Quantitative Trait Locus Disease Severity Index 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by the National Basic Research Program of China (973 Program 2009CB118400) and National High Technology Research and Development Program of China (863 Program) (No. 2012AA10A306). We thank Professor Deng-hai Li (Laizhou Academy of Agricultural Sciences) for donating maize inbred line ‘Ye478’ and supporting to field tests. We thank Professor Cheng-he Zhang (Hebei Academy of Agricultural Sciences) for donating maize inbred line ‘90110’. We are grateful to Doctor Roberta Greenwood for help with editing the English of the paper.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Junwen Luan
    • 1
  • Fei Wang
    • 1
    • 2
  • Yujie Li
    • 1
  • Bin Zhang
    • 1
  • Juren Zhang
    • 1
    Email author
  1. 1.School of Life SciencesShandong UniversityJinanChina
  2. 2.School of Life SciencesShanghai UniversityShanghaiChina

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