Theoretical and Applied Genetics

, Volume 125, Issue 3, pp 503–515 | Cite as

Combinations of mutant FAD2 and FAD3 genes to produce high oleic acid and low linolenic acid soybean oil

  • Anh-Tung Pham
  • J. Grover  Shannon
  • Kristin D. Bilyeu
Original Paper


High oleic acid soybeans were produced by combining mutant FAD2-1A and FAD2-1B genes. Despite having a high oleic acid content, the linolenic acid content of these soybeans was in the range of 4–6 %, which may be high enough to cause oxidative instability of the oil. Therefore, a study was conducted to incorporate one or two mutant FAD3 genes into the high oleic acid background to further reduce the linolenic acid content. As a result, soybean lines with high oleic acid and low linolenic acid (HOLL) content were produced using different sources of mutant FAD2-1A genes. While oleic acid content of these HOLL lines was stable across two testing environments, the reduction of linolenic acid content varied depending on the number of mutant FAD3 genes combined with mutant FAD2-1 genes, on the severity of mutation in the FAD2-1A gene, and on the testing environment. Combination of two mutant FAD2-1 genes and one mutant FAD3 gene resulted in less than 2 % linolenic acid content in Portageville, Missouri (MO) while four mutant genes were needed to achieve the same linolenic acid in Columbia, MO. This study generated non-transgenic soybeans with the highest oleic acid content and lowest linolenic acid content reported to date, offering a unique alternative to produce a fatty acid profile similar to olive oil.


Linolenic Acid Content Oleic Acid Content Soybean Line High Oleic Acid Palmitic Acid Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors wish to acknowledge excellent technical assistance provided by Paul Little, Christine Cole and Stewart Selves. Dr. Jeong-Dong Lee provided F1 seeds for Cross 1. Mention of a trademark, vendor, or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products or vendors that may also be suitable.


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Copyright information

© Springer-Verlag (outside the USA) 2012

Authors and Affiliations

  • Anh-Tung Pham
    • 1
  • J. Grover  Shannon
    • 2
  • Kristin D. Bilyeu
    • 3
  1. 1.Division of Plant SciencesUniversity of MissouriColumbiaUSA
  2. 2.Division of Plant Sciences, University of Missouri-Delta Research CenterUniversity of MissouriPortagevilleUSA
  3. 3.USDA/ARS, Plant Genetics Research UnitUniversity of MissouriColumbiaUSA

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