Theoretical and Applied Genetics

, Volume 125, Issue 3, pp 495–502 | Cite as

Quantitative trait loci responsible for Fusarium head blight resistance in Chinese landrace Baishanyuehuang

Original Paper
First Online:
Received:
Accepted:

Abstract

Fusarium head blight (FHB), mainly caused by Fusarium graminearum, is a destructive disease that can significantly reduce grain yield and quality. Deployment of quantitative trait loci (QTLs) for FHB resistance in commercial cultivars has been the most effective approach for minimizing the disease losses. ‘Baishanyuehuang’ is a highly FHB-resistant landrace from China. Recombinant inbred lines (RILs) developed from a cross of ‘Baishanyuehuang’ and ‘Jagger’ were evaluated for FHB resistance in three greenhouse experiments in 2010 and 2011 by single-floret inoculation. Percentage of symptomatic spikelets in an inoculated spike was recorded 18 days post-inoculation. The RIL population was screened with 251 polymorphic simple sequence repeats. Four QTLs were associated with FHB resistance and mapped on three chromosomes. Two QTLs were located on the short arm of chromosome 3B (3BS) with one in distal of 3BS and another near centromere (3BSc), designated as Qfhb.hwwg-3BSc. The QTL in the distal of 3BS is flanked by Xgwm533 and Xgwm493, thus corresponds to Fhb1. This QTL explained up to 15.7 % of phenotypic variation. Qfhb.hwwg-3BSc flanked by Xwmc307 and Xgwwm566 showed a smaller effect than Fhb1 and explained up to 8.5 % of phenotypic variation. The other two QTLs were located on 3A, designated as Qfhb.hwwg-3A, and 5A, designated as Qfhb.hwwg-5A. Qfhb.hwwg-3A was flanked by Xwmc651 and Xbarc356 and explained 4.8–7.5 % phenotypic variation, and Qfhb.hwwg-5A was flanked by markers Xgwm186 and Xbarc141, detected in only one experiment, and explained 4.5 % phenotypic variation for FHB resistance. ‘Baishanyuehuang’ carried all resistance alleles of the four QTL. Qfhb.hwwg-3BSc and Qfhb.hwwg-3A were new QTLs in ‘Baishanyuehuang’. ‘Baishanyuehuang’ carries a combination of QTLs from different sources and can be a new source of parent to pyramid FHB-resistant QTLs for improving FHB resistance in wheat.

Keywords

Simple Sequence Repeat Marker Fusarium Head Blight Composite Interval Mapping Fusarium Head Blight Resistance Simple Sequence Repeat Primer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

Notes

Acknowledgments

This project was partially supported by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture and USDA Wheat and Barley Scab Initiative. USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. This is contribution No. 12-093-J from the Kansas Agricultural Experiment Station, Manhattan, KS. X. Zhang was partially supported by China Scholarship Council.

Supplementary material

122_2012_1848_MOESM1_ESM.docx (323 kb)
Supplemental Fig. 1 A linkage map developed from the recombinant inbred line (RIL) population from ‘Baishanyuehuang’/‘Jagger’ (DOCX 323 kb)

References

  1. Anderson JA, Liu S (2007) Molecular breeding using a major QTL for Fusarium head blight resistance in wheat. Crop Sci 47(Suppl 3):S112–S119Google Scholar
  2. Anderson JA, Stack RW, Liu S, Waldron BL, Field AD, Coyne C, Moreno-Sevilla B, Mitchell FJ, Song QJ, Cregan PB, Frohberg RC (2001) DNA markers for Fusarium head blight resistance QTL in two wheat populations. Theor Appl Genet 102:1164–1168CrossRefGoogle Scholar
  3. Bai GH, Shaner G (1994) Scab of wheat: prospects for control. Plant Dis 78:760–766CrossRefGoogle Scholar
  4. Bai GH, Shaner G (2004) Management and resistance in wheat and barley to Fusarium head blight. Annu Rev Phytopathol 42:135–161PubMedCrossRefGoogle Scholar
  5. Bai GH, Kolb FL, Shaner G, Domier LL (1999) Amplified fragment length polymorphism markers linked to a major quantitative trait locus controlling scab resistance in wheat. Phytopathology 89:343–348PubMedCrossRefGoogle Scholar
  6. Bai GH, Shaner G, Ohm H (2000) Inheritance of resistance to Fusarium graminearum in wheat. Theor Appl Genet 100:1–8CrossRefGoogle Scholar
  7. Bernardo AN, Ma HX, Zhang D, Bai GH (2011) Single nucleotide polymorphism in wheat chromosome region harboring Fhb1 for Fusarium head blight resistance. Mol Breed. doi: 10.1007/s11032-011-9565-y
  8. Bourdoncle W, Ohm HW (2003) Quantitative trait loci for resistance to Fusarium head blight in recombinant inbred wheat lines from the cross Huapei 57–2/Patterson. Euphytica 131:131–136CrossRefGoogle Scholar
  9. Buerstmayr H, Lemmens M, Hartl L, Doldi L, Steiner B, Stierschneider M, Ruckenbauer P (2002) Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (type II resistance). Theor Appl Genet 104:84–91PubMedCrossRefGoogle Scholar
  10. Buerstmayr H, Steiner B, Hartl L, Griesser M, Angerer N, Lengauer D, Miedaner T, Schneider B, Lemmens M (2003) Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. II. Resistance to fungal penetration and spread. Theor Appl Genet 107:503–508PubMedCrossRefGoogle Scholar
  11. Chen XF, Faris JD, Hu JG, Stack RW, Adhikari T, Elias EM, Kianian SF, Cai XW (2007) Saturation and comparative mapping of a major Fusarium head blight resistance QTL in tetraploid wheat. Mol Breed 19:113–124CrossRefGoogle Scholar
  12. Desjardins AE, Hohn TM (1997) Mycotoxins in plant pathogenesis. Mol Plant Microbe Interact 10:147–152CrossRefGoogle Scholar
  13. Doerge RW, Churchill GA (1996) Permutation tests for multiple loci affecting a quantitative character. Genetics 142:285–294PubMedGoogle Scholar
  14. Gervais L, Dedryver F, Morlais JY, Bodusseau V, Negre S, Bilous M, Groos C, Trottet M (2003) Mapping of quantitative trait loci for field resistance to Fusarium head blight in an European winter wheat. Theor Appl Genet 106:961–970PubMedGoogle Scholar
  15. Lin F, Xue SL, Zhang ZZ, Zhang CQ, Kong ZX, Yao GQ, Tian DG, Zhu HL, Li CJ, Cao Y, Wei JB, Luo QY, Ma ZQ (2006) Mapping QTL associated with resistance to Fusarium head blight in the Nanda2419 × Wangshuibai population. II: type I resistance. Theor Appl Genet 112:528–535PubMedCrossRefGoogle Scholar
  16. Liu S, Abate ZA, Lu H, Mckendry AL (2007) QTL associated with Fusarium head blight resistance in the soft red winter wheat Ernie. Theor Appl Genet 115:417–427PubMedCrossRefGoogle Scholar
  17. Ma HX, Zhang KM, Gao L, Bai GH, Chen HG, Cai ZX, Lu WZ (2006) Quantitative trait loci for resistance to Fusarium head blight and deoxynivalenol accumulation in Wangshuibai wheat under field conditions. Plant Pathol 55:739–745CrossRefGoogle Scholar
  18. McCartney CA, Somers DJ, Fedak G, DePauw RM, Thomas J, Fox SL, Humphreys DG, Lukow O, Savard ME, McCallum BD (2007) The evaluation of FHB resistance QTLs introgressed into elite Canadian spring wheat germplasm. Mol Breed 20:209–221CrossRefGoogle Scholar
  19. Mesterhazy A (1995) Types and components of resistance to Fusarium head blight of wheat. Plant Breed 114:377–386CrossRefGoogle Scholar
  20. Paillard S, Schnurbusch T, Tiwari R, Messmer M, Winzeler M, Keller B, Schachermayr G (2004) QTL analysis of resistance to Fusarium head blight in Swiss winter wheat (Triticum aestivum L.). Theor Appl Genet 109:323–332PubMedCrossRefGoogle Scholar
  21. Pumphrey MO, Bernardo R, Anderson JA (2007) Validating the Fhb1 QTL for Fusarium head blight resistance in near-isogenic wheat lines developed from breeding populations. Crop Sci 47:200–206CrossRefGoogle Scholar
  22. Roder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  23. Ruckenbauer P, Buerstmayr H, Lemmens M (2001) Present strategies in resistance breeding against scab (Fusarium spp). Euphytica 119:121–127CrossRefGoogle Scholar
  24. Saghai-Maroof MA, Solima KM, Jorgenson RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018PubMedCrossRefGoogle Scholar
  25. Salameh A, Almaghrabi B, Buerstmayr H (2008) Development and evaluation of winter wheat breeding lines carrying Fusarium head blight resistance QTL derived from spring wheat. Cereal Res Commun 36:161–162Google Scholar
  26. Schroeder HW, Christensen JJ (1963) Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology 53:831–838Google Scholar
  27. Shen XR, Ittu M, Ohm HW (2003) Quantitative trait loci conditioning resistance to Fusarium head blight in wheat line F201R. Crop Sci 43:850–857CrossRefGoogle Scholar
  28. Snijders CHA (1994) Breeding for resistance to Fusarium in wheat and maize. In: Miller JD, Trenholm HL (eds) Mycotoxins in grain compounds other than aflatoxin. Eagan Press, St. Paul, pp 37–58Google Scholar
  29. Somers DJ, Fedak G, Savard M (2003) Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat. Genome 46:555–564PubMedCrossRefGoogle Scholar
  30. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114PubMedCrossRefGoogle Scholar
  31. Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J, Gill BS, Ward R, Cregan PB (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550–560PubMedCrossRefGoogle Scholar
  32. Steiner B, Griesser M, Scholz U, Schondelmaier J, Buerstmayr H (2004) Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana. Theor Appl Genet 109:215–224PubMedCrossRefGoogle Scholar
  33. Waldron BL, Moreno-Sevilla B, Anderson JA, Stack RW, Frohberg RC (1999) RFLP mapping of QTL for Fusarium head blight resistance in wheat. Crop Sci 39:805–811CrossRefGoogle Scholar
  34. Wang S, Basten CJ, Zeng ZB (2006) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh. http://statgen.ncsu.edu/qtlcart/WQTLCart.htm (verified 28 May 2008)
  35. Windels CE (2000) Economic and social impacts of Fusarium head blight: changing farms and rural communities in the northern Great Plains. Phytopathol 90:17–21CrossRefGoogle Scholar
  36. Yang ZP, Gilbert J, Fedak G, Somers DJ (2005a) Genetic characterization of QTL associated with resistance to Fusarium head blight in a doubled haploid spring wheat population. Genome 48:187–196PubMedCrossRefGoogle Scholar
  37. Yang J, Bai GH, Shaner GE (2005b) Novel quantitative trait loci (QTL) for Fusarium head blight resistance in wheat cultivar Chokwang. Theor Appl Genet 111:1571–1579PubMedCrossRefGoogle Scholar
  38. Yu JB, Bai GH, Cai SB, Dong YH, Ban T (2008a) New FHB-resistant sources from Asian wheat germplasm. Crop Sci 48:1090–1097CrossRefGoogle Scholar
  39. Yu JB, Bai GH, Zhou WC, Dong YH, Kolb FL (2008b) Quantitative trait loci for Fusarium head blight resistance in a recombinant inbred population of Wangshuibai/Wheaton. Phytopathology 98:87–94PubMedCrossRefGoogle Scholar
  40. Zhou WC, Kolb FL, Bai GH, Shaner G, Domier LL (2002) Genetic analysis of scab resistance QTL in wheat with microsatellite and AFLP markers. Genome 45:719–727PubMedCrossRefGoogle Scholar
  41. Zhou WC, Kolb FL, Bai GH, Domier LL, Boze LK, Smith NJ (2003) Validation of a major QTL for scab resistance with SSR markers and use of marker-assisted selection in wheat. Plant Breed 122:40–46CrossRefGoogle Scholar
  42. Zhou WC, Kolb FL, Yu JB, Bai GH, Boze LK, Domier LL (2004) Molecular characterization of Fusarium head blight resistance in Wangshuibai with simple sequence repeat and amplified fragment length polymorphism markers. Genome 47:1137–1143PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag (outside the USA) 2012

Authors and Affiliations

  1. 1.College of Plant ScienceJilin UniversityChangchunChina
  2. 2.Department of AgronomyKansas State UniversityManhattanUSA
  3. 3.USDA-ARS Hard Winter Wheat Genetics Research UnitManhattanUSA

Personalised recommendations