Comparative cytogenetic mapping between the lima bean (Phaseolus lunatus L.) and the common bean (P. vulgaris L.)
The common bean (Phaseolus vulgaris) and lima bean (P. lunatus) are among the most important legumes in terms of direct human consumption. The present work establishes a comparative cytogenetic map of P. lunatus, using previously mapped markers from P. vulgaris, in association with analyses of heterochromatin distribution using the fluorochromes chromomycin A3 (CMA) and 4′,6-diamidino-2-phenylindole (DAPI) and localization of the 5S and 45S ribosomal DNA (rDNA) probes. Seven BACs selected from different common bean chromosomes demonstrated a repetitive pericentromeric pattern corresponding to the heterochromatic regions revealed by CMA/DAPI and could not be mapped. The subtelomeric repetitive pattern observed for BAC 63H6 in most of the chromosome ends of common bean was not detected in lima bean, indicating lack of conservation of this subtelomeric repeat. All chromosomes could be identified and 16 single-copy clones were mapped. These results showed a significant conservation of synteny between species, although change in centromere position suggested the occurrence of pericentric inversions on chromosomes 2, 9 and 10. The low number of structural rearrangements reflects the karyotypic stability of the genus.
KeywordsBacterial Artificial Chromosome Common Bean Chromosome Pair Lima Bean Pericentric Inversion
We thank Heloisa Torres (EMBRAPA Arroz e Feijão) for the seeds, Dr. Paul Gepts (University of California) for the BAC clones and Valérie Geffroy (Université Paris Sud) for the B61 bacteriophage. C. A. was supported by a grant from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil. A. F. and K. G. B. dos S. were supported by grants from Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE), Brazil. M. M. was supported by the Gregor Mendel Institute of Molecular Plant Biology (GMI), Austria. The work was supported by CNPq, Brazil.
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