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Theoretical and Applied Genetics

, Volume 124, Issue 8, pp 1503–1512 | Cite as

Inheritance and genetic mapping of two nuclear genes involved in nuclear–cytoplasmic incompatibility in peas (Pisum sativum L.)

  • Vera S. Bogdanova
  • Elvira R. Galieva
  • Arseniy K. Yadrikhinskiy
  • Oleg E. KosterinEmail author
Original Paper

Abstract

Genetic analysis was performed to finely map and assess the mode of inheritance of two unlinked nuclear genes Scs1 and Scs2 involved in incompatibility of the nuclear genome of the cultivated pea Pisum sativum subsp. sativum with the cytoplasm of the wild pea of the subspecies P. sativum subsp. elatius, accession VIR320. Based on the segregation of genotypes in the progeny of the test-crosses, we concluded that if the cytoplasm was inherited from the wild pea VIR320, the Scs1 allele from the cultivated pea was gametophyte lethal and sporophyte recessive lethal. The Scs2 allele from the cultivated pea reduced male gametophyte viability. In homozygote, Scs2 from cultivated parent brought about nuclear–cytoplasmic conflict manifested as chlorophyll deficiency, reduction of blade organs, and low pollen fertility of about 20%. In heterozygote, Scs1 and Scs2 genes reduced pollen fertility by ca 50 and 30%, respectively. The Scs1 and Scs2 genes involved in nuclear–cytoplasmic incompatibility were genetically mapped. The distance between the markers bordering Scs1 comprised about 2.5 cM on linkage group III. The map distance between the bordering markers in the neighborhood of Scs2 varied substantially from cross to cross in the range of 2.0–15.1 cM on linkage group V.

Keywords

rbcL Test Line Pollen Count Pollen Fertility Male Gametophyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors are grateful to Dr. James J. Russo, from Columbia University for valuable suggestions and correcting the English language. This work was supported by the Russian Foundation for Fundamental Research, Grant 10-04-00230-a. Microscopic analysis was performed at the Microscopy Centre of the Institute of Cytology and Genetics SD RAS.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

122_2012_1804_MOESM1_ESM.pdf (204 kb)
Supplementary material 1 (PDF 203 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Vera S. Bogdanova
    • 1
  • Elvira R. Galieva
    • 1
  • Arseniy K. Yadrikhinskiy
    • 1
  • Oleg E. Kosterin
    • 1
    • 2
    Email author
  1. 1.Institute of Cytology and GeneticsRussian Academy of Sciences, Siberian BranchNovosibirskRussia
  2. 2.Novosibirsk State UniversityNovosibirskRussia

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