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Development of selective markers linked to a major QTL for parthenocarpy in eggplant (Solanum melongena L.)

Abstract

Parthenocarpy, the ability to set fruits without pollination, is a useful trait for setting fruit under unfavorable conditions. To identify the loci controlling parthenocarpy in eggplant (Solanum melongena L.), we constructed linkage maps by using co-dominant simple sequence repeat and single nucleotide polymorphism markers in F2 populations derived from intraspecific crosses between two non-parthenocarpic lines (LS1934 and Nakate-Shinkuro) and a parthenocarpic line (AE-P03). Total map distances were 1,414.6 cM (ALF2: LS1934 × AE-P03) and 1,153.8 cM (NAF2: Nakate-Shinkuro × AE-P03), respectively. Quantitative trait locus (QTL) analyses revealed two QTLs on chromosomes 3 and 8, which we denoted as Controlling parthenocarpy3.1 (Cop3.1) and Cop8.1, respectively. The percentage of phenotypic variance explained (PVE) of Cop3.1 was 6.3% in ALF2 (LOD = 4.2) and 10.6% in NAF2 (LOD = 3.0). The PVE of Cop8.1 was 45.7% in ALF2 (LOD = 23.8) and 29.7% in NAF2 (LOD = 7.9). Using a population of backcross inbred lines, we confirmed the effect of Cop8.1, but there was no evidence to support the contribution of Cop3.1. We need to verify the effect of Cop3.1 under various temperature conditions. In addition, we clarified the effectiveness of selective SSR markers, emf21H22 and emh11J10, mapped on each side of Cop8.1 in other F2 populations derived from various parental combinations. This is the first report concerning QTL analysis of parthenocarpy in eggplant using molecular markers. It will be useful in marker-assisted selection and in revealing the genomic mechanism underlying parthenocarpy in eggplant.

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Acknowledgments

We are grateful to Dr. M. Yano and Dr. T. Hayashi, National Institute of Agrobiological Sciences, for their suggestions for the construction of the genetic linkage map and QTL mapping. We are also grateful to Ms. H. Maeda, Ms. Y. Kitamura, and Ms. T. Tani for their technical assistance. This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Development of mitigation and adaptation techniques to global warming in the sectors of agriculture, forestry, and fisheries, C-3-1030).

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Correspondence to Koji Miyatake.

Additional information

Communicated by I. Paran.

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Miyatake, K., Saito, T., Negoro, S. et al. Development of selective markers linked to a major QTL for parthenocarpy in eggplant (Solanum melongena L.). Theor Appl Genet 124, 1403–1413 (2012). https://doi.org/10.1007/s00122-012-1796-8

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Keywords

  • Quantitative Trait Locus
  • Simple Sequence Repeat Marker
  • Quantitative Trait Locus Analysis
  • Single Nucleotide Polymorphism Marker
  • Phenotypic Variance Explain