Theoretical and Applied Genetics

, Volume 124, Issue 5, pp 903–909 | Cite as

Genetic characterization and fine mapping of a yellow-seeded gene in Dahuang (a Brassica rapa landrace)

  • Lu Xiao
  • Zhi Zhao
  • Dezhi Du
  • Yanmei Yao
  • Liang Xu
  • Guoyong Tang
Original Paper

Abstract

The development of yellow-seeded cultivars in Brassica rapa (B. rapa) would improve the quality and quantity of available oil. The identification and mapping of the seed coat color gene may aid in the development of yellow-seeded cultivars and facilitate introgression of the yellow-seeded gene into desirable Brassica napus (B. napus) lines through marker-assisted selection. In the current study, we investigated the inheritance of a yellow-seeded landrace in B. rapa, “Dahuang”, originating from the Qinghai-Tibetan plateau. Genetic analysis revealed that the phenotype of the yellow-seeded trait in Dahuang is controlled by one recessive gene, termed Brsc1. Mapping of the Brsc1 gene was subsequently conducted in a BC1 population comprised 456 individuals, derived from (Dahuang × 09A-126) × Dahuang. From a survey of 256 amplified fragment length polymorphism (AFLP) primer combinations, 10 tightly linked AFLP markers were obtained. The closest AFLP markers flanking Brsc1, Y10 and Y06, were 0.2 and 0.4 cM away, respectively. Subsequently, using simple sequence repeat (SSR) markers in the reference map, the Brsc1 gene was mapped on A09 in B. rapa. Blast analysis revealed that seven AFLP markers showed sequence homology to A09 of B. rapa, wherein six AFLP markers in our map were in the same order as those in A09 of B. rapa. The two closest markers, Y10 and Y06, delimited the Brsc1 gene within a 2.8 Mb interval. Furthermore, Y05 and Y06, the two closest AFLP markers on one side linked to Brsc1, were located in scaffold000059 on A09 of B. rapa, whereas the closet AFLP marker on the opposite side of Brsc1, Y10, was located in scaffold000081 on A09 of B. rapa. Molecular markers developed from these studies may facilitate marker-assisted selection (MAS) of yellow-seeded lines in B. rapa and B. napus and expedite the process of map-based cloning of Brsc1.

Keywords

B. rapa Seed coat color AFLP SSR Fine mapping 

Notes

Acknowledgments

The authors are grateful to Dr. Shaolin Lei and Dr. Zhen Huang for their critical reading of the manuscript. This research was financially supported by funds from the national nature science funds of China (31060196), the High-tech program “863” (2011AA10A104) and the National Key Basic Research Program of China “973”.

References

  1. Ahmed SU, Zuberi MI (1971) Inheritance of seed coat color in Brassica campestris L. variety Toria. Crop Sci 11:309–310CrossRefGoogle Scholar
  2. Badani AG, Snowdon RJ, Wittkop B, Lipsa FD, Baetzel R, Horn R (2006) Colocalization of a partially dominant gene for yellow seed colour with a major QTL influencing acid detergent fibre (ADF) content in different crosses of oilseed rape (Brassica napus). Genome 49:1499–1509PubMedCrossRefGoogle Scholar
  3. Chen W, Zhang Y, Liu XP, Chen BY, Tu JX, Fu TD (2007) Detection of QTL for six yield-related traits in oilseed rape (Brassica napus) using DH and immortalized F2 populations. Theor Appl Genet 115:849–858PubMedCrossRefGoogle Scholar
  4. Cheng XM, Xu JS, Xia S, Gu JX, Yang Y, Fu J, Qian XJ, Zhang SC, Wu JS, Liu KD (2009a) Development and genetic mapping of microsatellite markers from genome survey sequences in Brassica napus. Theor Appl Genet. doi: 10.1007/s00122-009-0967-8
  5. Cheng Y, Geng JF, Zhang JY, Wang Q, Ban QY, Hou XL (2009b) The construction of a genetic linkage map of non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino). J Genet Genomics 36:501–508PubMedCrossRefGoogle Scholar
  6. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
  7. He YT, Ma CZ, Ma Y, Meng LY, Tu JX, Fu TD (2003) Cloning of S-locus gene by PCR walking in Brassica campestris L. Oil Crop China 26(4):1–5Google Scholar
  8. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175CrossRefGoogle Scholar
  9. Lakshmi Padmaja K, Arumugam N, Gupta V, Mukhopadhyay A, Sodhi YS, Pental D, Pradhan AK (2005) Mapping and tagging of seed coat colour and the identification of microsatellite markers for marker-assisted manipulation of the trait in Brassica juncea. Theor Appl Genet 111:8–14PubMedCrossRefGoogle Scholar
  10. Lander E, Green P, Abrahamson J, Barlow A, Daley M, Lincoln S, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181PubMedCrossRefGoogle Scholar
  11. Lincoln S, Daly M, Lander E (1992) Constructing genetic linkage maps with Mapmaker/exp 3.0: a tutorial and reference manual, 3rd edn. Whitehead Institute Technical ReportGoogle Scholar
  12. Liu HL (1985) Rapeseed genetics and breeding. Shanghai Science and Technology Press, Shanghai, pp 510–549Google Scholar
  13. Liu HL (2000) Genetics and breeding in rapeseed. Chinese Agricultural University Press, Beijing, p 29Google Scholar
  14. Liu HL, Han JX, Hu XJ (1991) Studies on the inheritance of seed coat colour and other related characteristics of yellow seeded Brassica napus. In: Proceedings of the 8th international rapeseed congress, vol 5. Saskatoon, Canada, pp 1438–1444Google Scholar
  15. Liu ZW, Fu TD, Tu JX, Chen BY (2005) Inheritance of seed colour and identification of RAPD and AFLP markers linked to the seed colour gene in rapeseed (Brassica napus L.). Theor Appl Genet 110:303–310CrossRefGoogle Scholar
  16. Lowe AJ, Jones AE, Raybould AF, Trick M, Moule CJ, Edwards KJ (2002) Transferability and genome specificity of a new set of microsatellite primers among Brassica species of the U triangle. Mol Ecol Notes 2:7–11CrossRefGoogle Scholar
  17. Lu GY, Yang GS, Fu TD (2004) Molecular mapping of a dominant genic male sterility gene (Ms) in rapeseed (Brassica napus). Plant Breed 123:262–265CrossRefGoogle Scholar
  18. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci 88:9828–9832PubMedCrossRefGoogle Scholar
  19. Negi MS, Devic M, Delseny M, Lakshmikumaran M (2000) Identification of AFLP fragments linked to seed coat colour in Brassica juncea and conversion to SCAR marker for rapid selection. Theor Appl Genet 101:146–152CrossRefGoogle Scholar
  20. Piquemal J, Cinquin E, Couton F, Rondeau C, Seignoret E, doucet I, Perret D, Villeger MJ, Vincourt P, Blanchard P (2005) Construction of an oilseed rape (Brassica napus L.) genetic map with SSR markers. Theor Appl Genet 111:1514–1523PubMedCrossRefGoogle Scholar
  21. Rahman MH (2001) Production of yellow-seeded Brassica napus through interspecific crosses. Plant Breed 120:463–472CrossRefGoogle Scholar
  22. Rakow G, Relf-Eckstein J, Raney P, Gugel R (1999) Development of high yielding, disease resistant, yellow-seeded Brassica napus. In: Proceedings of the 10th international rapeseed congress, Canberra. http://www.regional.org.au/au/gcirc/4/68.htm. (Cited Jun 2010)
  23. Shirzadegan M, Röbellen G (1985) Influence of seed colour and hull proportion an quality properties of seeds in Brassica napus L. Fette Seifen Anstrichm 87:235–237CrossRefGoogle Scholar
  24. Stringam GR (1980) Inheritance of seed color in turnip rape. Can J Plant Sci 60:331–335CrossRefGoogle Scholar
  25. Stringman GR, McGregor DI, Pawlowski SH (1974) Chemical and morphological characteristics associated with seed coat color in rapeseed. In: 6th proceedings of 4th international rapeseed conference, Giessen, West Germany, pp 99–108Google Scholar
  26. Vera CL, Woods DL, Downey RK (1979) Inheritance of seed coat color in Brassica juncea. Can J Plant Sci 59:635–637CrossRefGoogle Scholar
  27. Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Freijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23:4407–4414PubMedCrossRefGoogle Scholar
  28. Xiao SS, Xu JS, Li Y, Zhang L, Shi SJ, Shi SW, Wu JS, Liu KD (2007) Generation and mapping of SCAR and CAPS markers linked to the seed coat color gene in Brassica napus using a genome-walking technique. Genome 50:611–618PubMedCrossRefGoogle Scholar
  29. Yi B, Chen YN, Lei SL, Tu JX, Fu TD (2006) Fine mapping of the recessive genic male-sterile gene (Bnms1) in Brassica napus L. Theor Appl Genet 113:643–650PubMedCrossRefGoogle Scholar
  30. Zhang JF, Lu Y, Yuan YX, Zhang XW, Geng JF, Chen Y, Cloutier S, McVetty PBE (2009) Li GY (2009) Map-based cloning and characterization of a gene controlling hairiness and seed coat color traits in Brassica rapa. Plant Mol Biol 69:553–563. doi: 10.1007/s11103-008-9437-y PubMedCrossRefGoogle Scholar
  31. Zhang Y, Li X, Chen W, Yi B, Wen J, Shen JX, Ma CZ, Chen BY, Tu JX, Fu TD (2010) Identification of two major QTL for yellow seed color in two crosses of resynthesized Brassica napus line no. 2127-17. Mol Breed. doi: 10.1007/s11032-010-9486-1

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Lu Xiao
    • 1
  • Zhi Zhao
    • 1
  • Dezhi Du
    • 1
  • Yanmei Yao
    • 1
  • Liang Xu
    • 1
  • Guoyong Tang
    • 1
  1. 1.Key Laboratory of Spring Rape Genetic Improvement of Qinghai Province, National Key Laboratory Breeding Base for Innovation and Utilization of Plateau Crop GermplasmQinghai Academy of Agricultural and Forestry SciencesXiningChina

Personalised recommendations