Theoretical and Applied Genetics

, Volume 124, Issue 5, pp 903–909 | Cite as

Genetic characterization and fine mapping of a yellow-seeded gene in Dahuang (a Brassica rapa landrace)

  • Lu Xiao
  • Zhi Zhao
  • Dezhi Du
  • Yanmei Yao
  • Liang Xu
  • Guoyong Tang
Original Paper
First Online:
Received:
Accepted:

Abstract

The development of yellow-seeded cultivars in Brassica rapa (B. rapa) would improve the quality and quantity of available oil. The identification and mapping of the seed coat color gene may aid in the development of yellow-seeded cultivars and facilitate introgression of the yellow-seeded gene into desirable Brassica napus (B. napus) lines through marker-assisted selection. In the current study, we investigated the inheritance of a yellow-seeded landrace in B. rapa, “Dahuang”, originating from the Qinghai-Tibetan plateau. Genetic analysis revealed that the phenotype of the yellow-seeded trait in Dahuang is controlled by one recessive gene, termed Brsc1. Mapping of the Brsc1 gene was subsequently conducted in a BC1 population comprised 456 individuals, derived from (Dahuang × 09A-126) × Dahuang. From a survey of 256 amplified fragment length polymorphism (AFLP) primer combinations, 10 tightly linked AFLP markers were obtained. The closest AFLP markers flanking Brsc1, Y10 and Y06, were 0.2 and 0.4 cM away, respectively. Subsequently, using simple sequence repeat (SSR) markers in the reference map, the Brsc1 gene was mapped on A09 in B. rapa. Blast analysis revealed that seven AFLP markers showed sequence homology to A09 of B. rapa, wherein six AFLP markers in our map were in the same order as those in A09 of B. rapa. The two closest markers, Y10 and Y06, delimited the Brsc1 gene within a 2.8 Mb interval. Furthermore, Y05 and Y06, the two closest AFLP markers on one side linked to Brsc1, were located in scaffold000059 on A09 of B. rapa, whereas the closet AFLP marker on the opposite side of Brsc1, Y10, was located in scaffold000081 on A09 of B. rapa. Molecular markers developed from these studies may facilitate marker-assisted selection (MAS) of yellow-seeded lines in B. rapa and B. napus and expedite the process of map-based cloning of Brsc1.

Keywords

B. rapa Seed coat color AFLP SSR Fine mapping 
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Notes

Acknowledgments

The authors are grateful to Dr. Shaolin Lei and Dr. Zhen Huang for their critical reading of the manuscript. This research was financially supported by funds from the national nature science funds of China (31060196), the High-tech program “863” (2011AA10A104) and the National Key Basic Research Program of China “973”.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Lu Xiao
    • 1
  • Zhi Zhao
    • 1
  • Dezhi Du
    • 1
  • Yanmei Yao
    • 1
  • Liang Xu
    • 1
  • Guoyong Tang
    • 1
  1. 1.Key Laboratory of Spring Rape Genetic Improvement of Qinghai Province, National Key Laboratory Breeding Base for Innovation and Utilization of Plateau Crop GermplasmQinghai Academy of Agricultural and Forestry SciencesXiningChina

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