Molecular mapping of Arabidopsis thaliana lipid-related orthologous genes in Brassica napus
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Quantitative Trait Loci (QTL) for oil content has been previously analyzed in a SG-DH population from a cross between a Chinese cultivar and a European cultivar of Brassica napus. Eight QTL with additive and epistatic effects, and with environmental interactions were evaluated. Here we present an integrated linkage map of this population predominantly based on informative markers derived from Brassica sequences, including 249 orthologous A. thaliana genes, where nearly half (112) are acyl lipid metabolism related genes. Comparative genomic analysis between B. napus and A. thaliana revealed 33 colinearity regions. Each of the conserved A. thaliana segments is present two to six times in the B. napus genome. Approximately half of the mapped lipid-related orthologous gene loci (76/137) were assigned in these conserved colinearity regions. QTL analysis for seed oil content was performed using the new map and phenotypic data from 11 different field trials. Nine significant QTL were identified on linkage groups A1, A5, A7, A9, C2, C3, C6 and C8, together explaining 57.79% of the total phenotypic variation. A total of 14 lipid related candidate gene loci were located in the confidence intervals of six of these QTL, of which ten were assigned in the conserved colinearity regions and felled in the most frequently overlapped QTL intervals. The information obtained from this study demonstrates the potential role of the suggested candidate genes in rapeseed kernel oil accumulation.
KeywordsQuantitative Trait Locus Cleave Amplify Polymorphic Sequence Significant Quantitative Trait Locus Sequence Characterize Amplify Region Marker Stable Quantitative Trait Locus
The authors are grateful to Dr. Christian Moellers and Prof. Heiko Becker (University of Gottingen), and Dr Zhonghua Chen (University of Western Sydney) for their critical reading of the manuscript and to Dr. Wolfgang Ecke (University of Gottingen) for his providing the original SSR marker names from AAFC consortium. Thanks also to Drs. Liu Jing, Wang Lei and Shengyi Liu (CAAS) for offering marker primers, siRNAs precursor and BAC sequences. Prof. Weijun Zhou (Zhejiang University) for kindly providing the rapeseed line “Gaoyou”. This research was financially supported by National Basic Research Programs (2006CB101601), National High-tech R&D Program (2011AA10A104), National Natural Science Foundations of China (No. 30871543, 31171180) and Zhejiang Provincial Natural Science Foundation of China (Z3100592).
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