Theoretical and Applied Genetics

, Volume 123, Issue 8, pp 1433–1443 | Cite as

Stable progeny production of the amphidiploid resynthesized Brassica napus cv. Hanakkori, a newly bred vegetable

  • K. Fujii
  • N. Ohmido
Original Paper


Resynthesized Brassica napus cv. Hanakkori (AACC, 2n = 38) was produced by cross-hybridization between B. rapa (AA, 2n = 20) and B. oleracea (CC, 2n = 18) as a new vegetative crop. Many studies have provided evidences for the instability and close relationship between A and C genome in the resynthesized B. napus cultivars. In fact, seed produced to obtain progeny in Hanakkori had unstable morphological characters and generated many off-type plants. In this study, we investigated the pollen fertility, chromosome number, structure, and behavior linked to various Hanakkori phenotypes to define factors of unstable phenotypic expression in the progeny. Hanakkori phenotypes were categorized into five types. The results of pollen fertility, chromosome number, and fluorescence in situ hybridization analysis for somatic mitosis cells indicated that the off-type plants had lower pollen fertility, aberrant chromosome number, and structures with small chromosome fragments. Observation of chromosomes at meiosis showed that the meiotic division in off-type plants led to appreciably higher abnormalities than in on-type plants. However, polyvalent chromosomes were observed frequently in both on- and off-type plants in diplotene stage of meiosis. We assume that the unstable morphological characters in resynthesized progeny were the result of abnormal division in meiosis. It results as important that the plants of normal phenotype, chromosome structure and minimized abnormal meiosis are selected to stabilize progeny.


Chromosome Number Pollen Fertility rDNA Locus Chromosome Fragment Meiosis Stage 
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We are very grateful to Dr. Ki-Byung Lim (Brassica Genome Team, National Institute of Agricultural Biotechnology, Rural Development Administration, Korea), for providing the CentBr2 probe. This research was also supported by the regional agriculture research funding in Yamaguchi prefectural Technology Center for Agriculture and Forestry, Japan. This was supported by Female researcher training system: reform and acceleration funding in Science and Technology Promotion Funding.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Yamaguchi Prefectural Technology Center for Agriculture and ForestryYamaguchiJapan
  2. 2.Graduate School of Human Development and EnvironmentKobe UniversityKobeJapan

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