Theoretical and Applied Genetics

, Volume 123, Issue 8, pp 1341–1357 | Cite as

Nucleotide diversity of a genomic sequence similar to SHATTERPROOF (PvSHP1) in domesticated and wild common bean (Phaseolus vulgaris L.)

  • L. Nanni
  • E. Bitocchi
  • E. Bellucci
  • M. Rossi
  • D. Rau
  • G. Attene
  • P. Gepts
  • R. Papa
Original Paper

Abstract

Evolutionary studies in plant and animal breeding are aimed at understanding the structure and organization of genetic variations of species. We have identified and characterized a genomic sequence in Phaseolus vulgaris of 1,200 bp (PvSHP1) that is homologous to SHATTERPROOF-1 (SHP1), a gene involved in control of fruit shattering in Arabidopsis thaliana. The PvSHP1 fragment was mapped to chromosome Pv06 in P. vulgaris and is linked to the flower and seed color gene V. Amplification of the PvSHP1 sequence from the most agronomically important legume species showed a high degree of interspecies diversity in the introns within the Phaseoleae, while the coding region was conserved across distant taxa. Sequencing of the PvSHP1 sequence in a sample of 91 wild and domesticated genotypes that span the geographic distribution of this species in the centers of origin showed that PvSHP1 is highly polymorphic and, therefore, particularly useful to further investigate the origin and domestication history of P. vulgaris. Our data confirm the gene pool structure seen in P. vulgaris along with independent domestication processes in the Andes and Mesoamerica; they provide additional evidence for a single domestication event in Mesoamerica. Moreover, our results support the Mesoamerican origin of this species. Finally, we have developed three indel-spanning markers that will be very useful for bean germplasm characterization, and particularly to trace the distribution of the domesticated Andean and Mesoamerican gene pools.

Supplementary material

122_2011_1671_MOESM1_ESM.xls (54 kb)
Supplementary material descriptive captions Table A1. Genotypes of Phaseolus spp. and other legumes used in this study. (XLS 54 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • L. Nanni
    • 1
  • E. Bitocchi
    • 1
  • E. Bellucci
    • 1
  • M. Rossi
    • 1
  • D. Rau
    • 2
  • G. Attene
    • 2
  • P. Gepts
    • 3
  • R. Papa
    • 1
    • 4
  1. 1.Dipartimento di Scienze Agrarie, Alimentari ed AmbientaliUniversità Politecnica delle MarcheAnconaItaly
  2. 2.Dipartimento di Scienze Agronomiche e Genetica Vegetale AgrariaUniversità degli Studi di SassariSassariItaly
  3. 3.Department of Plant Sciences/MS1Section of Crop and Ecosystem SciencesDavisUSA
  4. 4.Cereal Research Centre, Agricultural Research Council (CRA-CER)FoggiaItaly

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