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Theoretical and Applied Genetics

, 123:1215 | Cite as

Genetic map of artichoke × wild cardoon: toward a consensus map for Cynara cardunculus

  • Gabriella Sonnante
  • Angela Gatto
  • Anita Morgese
  • Francesco Montemurro
  • Giulio Sarli
  • Emanuela Blanco
  • Domenico Pignone
Original Paper

Abstract

An integrated consensus linkage map is proposed for globe artichoke. Maternal and paternal genetic maps were constructed on the basis of an F1 progeny derived from crossing an artichoke genotype (Mola) with its progenitor, the wild cardoon (Tolfa), using EST-derived SSRs, genomic SSRs, AFLPs, ten genes, and two morphological traits. For most genes, mainly belonging to the chlorogenic acid pathway, new markers were developed. Five of these were SNP markers analyzed through high-resolution melt technology. From the maternal (Mola) and paternal (Tolfa) maps, an integrated map was obtained, containing 337 molecular and one morphological markers ordered in 17 linkage groups (LGs), linked between Mola and Tolfa. The integrated map covers 1,488.8 cM, with an average distance of 4.4 cM between markers. The map was aligned with already existing maps for artichoke, and 12 LGs were linked via 31 bridge markers. LG numbering has been proposed. A total of 124 EST-SSRs and two genes were mapped here for the first time, providing a framework for the construction of a functional map in artichoke. The establishment of a consensus map represents a necessary condition to plan a complete sequencing of the globe artichoke genome.

Keywords

Linkage Group Chlorogenic Acid Cetyl Trimethyl Ammonium Bromide Flower Head Globe Artichoke 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We are grateful to Giuseppe Sonnante and Donatella Danzi for technical assistance. This work was partially supported by MiPAAF (Ministero delle Politiche Agricole Alimentari e Forestali), project “CarVarVi—Valorizzazione di germoplasma di carciofo attraverso la costituzione varietale ed il risanamento da virus” and by MiPAAF, project “Implementazione nazionale trattato FAO risorse genetiche vegetali”.

Supplementary material

122_2011_1661_MOESM1_ESM.docx (84 kb)
Supplementary material 1 (DOC 84 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Gabriella Sonnante
    • 1
  • Angela Gatto
    • 1
  • Anita Morgese
    • 1
  • Francesco Montemurro
    • 1
  • Giulio Sarli
    • 1
  • Emanuela Blanco
    • 1
  • Domenico Pignone
    • 1
  1. 1.Institute of Plant GeneticsNational Research CouncilBariItaly

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