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Theoretical and Applied Genetics

, Volume 123, Issue 5, pp 793–802 | Cite as

A novel FAD2-1 A allele in a soybean plant introduction offers an alternate means to produce soybean seed oil with 85% oleic acid content

  • Anh-Tung Pham
  • Jeong-Dong Lee
  • J. Grover Shannon
  • Kristin D. Bilyeu
Original Paper

Abstract

The alteration of fatty acid profiles in soybean to improve soybean oil quality has been a long-time goal of soybean researchers. Soybean oil with elevated oleic acid is desirable because this monounsaturated fatty acid improves the nutrition and oxidative stability of soybean oil compared to other oils. In the lipid biosynthetic pathway, the enzyme fatty acid desaturase 2 (FAD2) is responsible for the conversion of oleic acid precursors to linoleic acid precursors in developing soybean seeds. Two genes encoding FAD2-1A and FAD2-1B were identified to be expressed specifically in seeds during embryogenesis and have been considered to hold an important role in controlling the seed oleic acid content. A total of 22 soybean plant introduction (PI) lines identified to have an elevated oleic acid content were characterized for sequence mutations in the FAD 2-1A and FAD2-1B genes. PI 603452 was found to contain a deletion of a nucleotide in the second exon of FAD2-1A. These important SNPs were used in developing molecular marker genotyping assays. The assays appear to be a reliable and accurate tool to identify the FAD 2-1A and FAD2-1B genotype of wild-type and mutant plants. PI 603452 was subsequently crossed with PI 283327, a soybean line that has a mutation in FAD2-1B. Interestingly, soybean lines carrying both homozygous insertion/deletion mutation (indel) FAD2-1A alleles and mutant FAD2-1B alleles have an average of 82–86% oleic acid content, compared to 20% in conventional soybean, and low levels of linoleic and linolenic acids. The newly identified indel mutation in the FAD2-1A gene offers a simple method for the development of high oleic acid commercial soybean varieties.

Keywords

Oleic Acid Plant Introduction Oleic Acid Content Soybean Line High Oleic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors wish to acknowledge the excellent technical assistance provided by Paul Little, Christine Cole and Stewart Selves.

Conflict of interest

None.

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Copyright information

© Springer-Verlag (outside the USA) 2011

Authors and Affiliations

  • Anh-Tung Pham
    • 1
  • Jeong-Dong Lee
    • 2
  • J. Grover Shannon
    • 3
  • Kristin D. Bilyeu
    • 4
  1. 1.Division of Plant SciencesUniversity of MissouriColumbiaUSA
  2. 2.Division of Plant BiosciencesKyungpook National UniversityDaeguRepublic of Korea
  3. 3.Division of Plant SciencesUniversity of Missouri, University of Missouri-Delta Research CenterPortagevilleUSA
  4. 4.USDA/ARS, Plant Genetics Research UnitUniversity of MissouriColumbiaUSA

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