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Theoretical and Applied Genetics

, Volume 123, Issue 4, pp 527–544 | Cite as

Resistance to Soil-borne cereal mosaic virus in durum wheat is controlled by a major QTL on chromosome arm 2BS and minor loci

  • Marco Maccaferri
  • Claudio Ratti
  • Concepcion Rubies-Autonell
  • Victor Vallega
  • Andrea Demontis
  • Sandra Stefanelli
  • Roberto Tuberosa
  • Maria Corinna SanguinetiEmail author
Original Paper

Abstract

Soil-borne cereal mosaic (SBCM) is a viral disease, which seriously affects hexaploid as well as tetraploid wheat crops in Europe. In durum wheat (Triticum durum Desf.), the elite germplasm is characterized by a wide range of responses to SBCMV, from susceptibility to almost complete resistance. In this study, the genetic analysis of SBCMV resistance was carried out using a population of 181 durum wheat recombinant inbred lines (RILs) obtained from Meridiano (resistant) × Claudio (moderately susceptible), which were profiled with SSR and DArT markers. The RILs were characterized for SBCMV response in the field under severe and uniform SBCMV infection during 2007 and 2008. A wide range of disease reactions (as estimated by symptom severity and DAS-ELISA) was observed. A large portion of the variability for SBCMV response was explained by a major QTL (QSbm.ubo-2BS) located in the distal telomeric region of chromosome 2BS near the marker triplet Xbarc35Xwmc661Xgwm210, with R 2 values ranging from 51.6 to 91.6%. The favorable allele was contributed by Meridiano. Several QTLs with minor effects on SBCMV response were also detected. Consistently with the observed transgressive segregation, the resistance alleles at minor QTLs were contributed by both parents. The presence and effects of QSbm.ubo-2BS were validated through association mapping in a panel of 111 elite durum wheat accessions.

Keywords

Normalize Difference Vegetation Index Durum Wheat Association Mapping Composite Interval Mapping Grain Yield 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The research was supported by the Emilia-Romagna Region, the CARISBO Bank Foundation (Genomica Grano Duro Project) and by the Italian Minister for Education, University and Research (MIUR), FISR Project “Sistemi, metodologie e strategie per la caratterizzazione e valorizzazione della granella e degli alimenti derivati del frumento duro in ambienti marginali e/o vocazionali”.

Supplementary material

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Supplementary material 1 (PPT 243 kb)
122_2011_1605_MOESM2_ESM.doc (810 kb)
Supplementary material 2 (DOC 810 kb)
122_2011_1605_MOESM3_ESM.ppt (268 kb)
Supplementary material 3 (PPT 268 kb)

References

  1. Barbosa MM, Goulart LR, Prestes AM, Juliatti FC (2001) Genetic control of resistance to soil-borne wheat mosaic virus in Brazilian cultivars of Triticum aestivum L. Thell. Euphytica 122:417–422CrossRefGoogle Scholar
  2. Bass C, Hendley R, Adams MJ, Hammond-Kosack KE, Kanyuka K (2006) The Sbm1 locus conferring resistance to soil-borne cereal mosaic virus maps to a gene-rich region on 5DL in wheat. Genome 49:1140–1148PubMedCrossRefGoogle Scholar
  3. Bayles R, O’Sullivan D, Lea V, Freeman S, Budge G, Walsh K (2007) Controlling soil-borne cereal mosaic virus in the UK by developing resistant wheat cultivars. HGCA Project 2616. HGCA Crop Research News 32: Project Report no. 418Google Scholar
  4. Bonnefoy M, Boursereau J, Chesneau R (1994) Comportment des variétés face aux virus de la mosaique du blé et de la mosaique jaune du blé. Proceedings, Workshop on Mosaics of cereals transmitted by Polymyxa graminis Led., Blois, France, 7–8 April 1994, 57–62Google Scholar
  5. Bradbury PJ, Zhang Z, Kroon DE, Casstevens TM, Ramdoss Y, Buckler ES (2007) TASSEL: software for association mapping of complex traits in diverse samples. Bioinformatics 23:2633–2635PubMedCrossRefGoogle Scholar
  6. Breseghello F, Sorrells ME (2006) Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars. Genetics 172:1165–1177PubMedCrossRefGoogle Scholar
  7. Budge GE, Loram J, Donovan G, Boonham N (2008a) RNA2 of Soil-borne cereal mosaic virus is detectable in plants of winter wheat grown from infected seeds. Eur J Plant Pathol 120:97–102CrossRefGoogle Scholar
  8. Budge GE, Ratti C, Rubies-Autonell C, Lockley D, Bonnefoy M, Vallega V, Pietravalle S, Henry CM (2008b) Response of UK winter wheat cultivars to Soil-borne cereal mosaic and Wheat spindle streak mosaic viruses across Europe. Eur J Plant Pathol 120:259–272CrossRefGoogle Scholar
  9. Canova A (1966) Ricerche sulle malattie da virus delle graminaceae. III Polymyxa graminis Led. vettore del mosaico del frumento. Phytopatol Medit 5:53–58Google Scholar
  10. Chao S, Zhang W, Dubcovsky J, Sorrells M (2007) Evaluation of genetic diversity and genome-wide linkage disequilibrium among US wheat (Triticum aestivum L.) germplasm representing different market classes. Crop Sci 47:1018–1030CrossRefGoogle Scholar
  11. Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971PubMedGoogle Scholar
  12. Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for detection of plant viruses. J Virol Meth 34:475–483Google Scholar
  13. Clover G, Wright D, Henry C (1999) Occurrence of Soil-borne wheat mosaic virus (SBWMV) in the United Kingdom. Proceedings, fourth symposium of the international working group on plant viruses with fungal vectors. Monterey, USA, 5–8 October 1999, 105–108Google Scholar
  14. Comadran J, Thomas WT, van Eeuwijk FA, Ceccarelli S, Grando S, Stanca AM, Pecchioni N, Akar T, Al-Yassin A, Benbelkacem A, Ouabbou H, Bort J, Romagosa I, Hackett CA, Russell JR (2009) Patterns of genetic diversity and linkage disequilibrium in a highly structured Hordeum vulgare association-mapping population for the Mediterranean basin. Theor Appl Genet 119:175–187PubMedCrossRefGoogle Scholar
  15. Conley EJ, Nduati V, Gonzalez-Hernandez JL, Mesfin A, Trudeau-Spanjers M, Chao S, Lazo GR, Hummel DD, Anderson OD, Qi LL, Gill BS, Echalier B, Linkiewicz AM, Dubcovsky J, Akhunov ED, Dyorak J, Peng JH, Lapitan NLV, Pathan MS, Nguyen HT, Ma XF, Miftahudin, Gustafson JP, Greene RA, Sorrells ME, Hossain G, Kalavacharla V, Kianian SF, Sidhu K, Dijbirligi M, Gill KS, Choi DW, Fenton RD, Close TJ, McGuire PE, Qualset CO, Anderson JA (2004) A 2600-locus chromosome bin map of wheat homoeologous group 2 reveals interstitial gene-rich islands and colinearity with rice. Genetics 168:625–637PubMedCrossRefGoogle Scholar
  16. Desiderio E, Belocchi A, D’Egidio MG, Fornara M, Cecchi V, Cecchini C (2007) Semina 2007: quali varietà di frumento duro scegliere. L’Informatore Agrario 34:5–6Google Scholar
  17. Diao A, Chen J, Ye R, Zheng T, Yu S, Antoniw JF, Adams MJ (1999) Complete sequence and genome properties of Chinese wheat mosaic virus, a new furovirus from China. J Gen Virol 80:1141–1145PubMedGoogle Scholar
  18. Dubey SN, Brown CM, Hooker AL (1970) Inheritance of field reaction to soil borne wheat mosaic virus. Crop Sci 10:93–95CrossRefGoogle Scholar
  19. Ersoz ES, Yu J, Buckler ES (2007) Applications of linkage disequilibrium and association mapping in crop plants. In: Varshney RK, Tuberosa R (eds) Genomics assisted crop improvement, vol 1: genomics approaches and platforms. Springer, Dordrecht, pp 97–119CrossRefGoogle Scholar
  20. Estes AP, Brakke MK (1966) Correlation of Polymyxa graminis with transmission of Soil-borne wheat mosaic virus. Virology 28:772–774PubMedCrossRefGoogle Scholar
  21. Eujayl ME, Sorrells M, Baum P, Wolters W, Powell W (2002) Isolation of EST-derived microsatellite markers for genotyping the A and B genomes of wheat. Theor Appl Genet 10:399–407CrossRefGoogle Scholar
  22. Farnir F, Coppieters W, Arranz JJ, Berzi P, Cambisano N, Grisart B, Karim L, Marcq F, Moreau L, Mni M, Nezer C, Simon P, Vanmanshoven P, Wagenaar D, Georges M (2000) Extensive genome-wide linkage disequilibrium in cattle. Minimal linkage disequilibrium in human Xq25 and Xq28. Genome Res 10:220–227PubMedCrossRefGoogle Scholar
  23. Giunta F, Motzo R, Pruneddu G (2007) Trends since 1900 in the yield potential of italian-bred durum cultivars. Eur J Agro 27:12–24CrossRefGoogle Scholar
  24. Gutiérrez AG, Carabalí SJ, Giraldo OX, Martínez CP, Correa F, Prado G, Joe Tohme J, Lorieux M (2010) Identification of a rice stripe necrosis virus resistance locus and yield component QTLs using Oryza sativa × O. glaberrima introgression lines. BMC Plant Biol 10:6. doi: 10.1186/1471-2229-10-6 PubMedCrossRefGoogle Scholar
  25. Hall MD, Brown-Guedira G, Klatt A, Fritz AK (2009) Genetic analysis of resistance to soil-borne wheat mosaic virus derived from Aegilops tauschii. Euphitica 169:169–176CrossRefGoogle Scholar
  26. Heffner EL, Sorrels ME, Jannink JL (2009) Genomic selection for crop improvement. Crop Sci 49:1–12CrossRefGoogle Scholar
  27. Jesewska M (1994) Identification and some properties of wheat soil-borne mosaic virus isolated in Poland. Phytopathol Polon 8:97–102Google Scholar
  28. Kanyuka K, Ward E, Adams MJ (2003) Polymyxa graminis and the cereal viruses it transmits: a research challenge. Mol Plant Pathol 4:393–406PubMedCrossRefGoogle Scholar
  29. Korzun V, Röder MS, Wendehake K, Pasqualone A, Lotti C, Ganal MW, Blanco A (1999) Integration of dinucleotide microsatellites from hexaploid bread wheat into a genetic linkage map of durum wheat. Theor Appl Genet 98:1202–1207CrossRefGoogle Scholar
  30. Kucharek TA, Walker JH (1974) The presence of and damage caused by Soil-borne wheat mosaic virus in Florida. Plant Dis Report 58:763–765Google Scholar
  31. Kühne T (2009) Soil-borne viruses affecting cereals—known for long but still a threat. Virus Res 141:174–183PubMedCrossRefGoogle Scholar
  32. Maccaferri M, Sanguineti MC, Donini P, Tuberosa R (2003) Microsatellite analysis reveals a progressive widening of the genetic basis in the elite durum wheat germplasm. Theor Appl Genet 107:783–797PubMedCrossRefGoogle Scholar
  33. Maccaferri M, Sanguineti MC, Tuberosa R (2005) Analysis of linkage disequilibrium in a collection of elite durum wheat genotypes. Mol Breeding 15:271–289CrossRefGoogle Scholar
  34. Maccaferri M, Sanguineti MC, Natoli V, Araus Ortega JL, Ben Salem M, Bort J, Chenenaoui C, De Ambrogio E, Garcia del Moral L, Demontis A, El-Ahmed A, Maalouf F, Machlab H, Moragues M, Motawaj J, Nachit M, Nserallah N, Ouabbou H, Royo C, Tuberosa R (2006) A panel of elite accessions of durum wheat (Triticum durum Desf.) suitable for association mapping studies. Plant Genet Resour 4:79–85CrossRefGoogle Scholar
  35. Maccaferri M, Stefanelli S, Rotondo F, Tuberosa R, Sanguineti MC (2007) Relationships among durum wheat accessions. I. Comparative analysis of SSR, AFLP, and phenotypic data. Genome 50:373–384PubMedCrossRefGoogle Scholar
  36. Maccaferri M, Mantovani P, Tuberosa R, DeAmbrogio E, Giuliani S, Demontis A, Massi A, Sanguineti MC (2008a) A major QTL for durable leaf rust resistance widely exploited in durum wheat breeding programs maps on the distal region of chromosome arm 7BL. Theor Appl Genet 117:1225–1240PubMedCrossRefGoogle Scholar
  37. Maccaferri M, Sanguineti MC, Corneti S, Araus Ortega JL, Ben Salern M, Bort J, DeAmbrogio E, Garcia del Moral L, Demontis A, El-Ahmed A, Maalouf F, Machlab H, Martos V, Moragues M, Motawaj J, Nachit M, Nserallah N, Ouabbou H, Royo C, Slama A, Tuberosa R (2008b) Quantitative trait loci for grain yield and adaptation of durum wheat (Triticum durum Desf.) across a wide range of water availability. Genetics 178:489–511PubMedCrossRefGoogle Scholar
  38. Maccaferri M, Ratti C, Rubies-Autonell C, Tuberosa R, Demontis A, Massi A, Stefanelli S, Vallega V, Sanguineti MC (2008c) Mapping genetic factors for resistance to Soil-Borne cereal mosaic virus (SBCMV) in durum wheat. 11th international wheat genetics symposium. Brisbane, Australia. 24–29 August, 2008Google Scholar
  39. Mantovani P, Maccaferri M, Sanguineti MC, Tuberosa R, Catizone I, Wenzl P, Thomson B, Carling J, Kilian A (2008) An integated DArT-SSR linkage map of durum wheat. Mol Breeding 22:629–648CrossRefGoogle Scholar
  40. Marti J, Bort J, Slafer GA, Araus JL (2007) Can wheat yield be assessed by early measurements of normalized difference vegetation index? Ann Appl Biol 150:253–257CrossRefGoogle Scholar
  41. McKinney HH (1923) Investigations of the rosette disease of wheat and its control. J Agric Res 23:771–800Google Scholar
  42. Merkle OG, Smith EL (1983) Inheritance of resistance to soil-borne mosaic in wheat. Crop Sci 23:1075–1076CrossRefGoogle Scholar
  43. Modawi RS, Heyne EG, Brunetta D, Willis WG (1982) Genetic studies of field reaction to wheat soil-borne mosaic virus. Plant Dis 66:1183–1184CrossRefGoogle Scholar
  44. Nakagawa M, Soga Y, Watanabe S, Gocho H, Nishio K (1959) Genetical studies on the wheat mosaic virus II. Genes affecting the inheritance of susceptibility to strains of yellow mosaic virus in varietal crosses of wheat. Jpn J Breed 9:118–120Google Scholar
  45. Narasimhamoorthy B, Gill BS, Fritz AK, Nelson JC, Brown-Guedira GL (2006) Advanced backcross QTL analysis of a hard winter wheat × synthetic wheat population. Theor Appl Genet 112:787–796PubMedCrossRefGoogle Scholar
  46. Perovic D, Forster J, Devaux P, Hariri D, Guilleroux M, Kanyuka K, Lyons R, Weyen J, Feuerhelm D, Kastirr U, Sourdille P, Röder M, Ordon F (2009) Mapping and diagnostic marker development for Soil-borne cereal mosaic virus resistance in bread wheat. Mol Breed 23:641–653CrossRefGoogle Scholar
  47. Ratti C, Pisi A, Vallega V, Rubies Autonell C (2005) Molecular characterization of Italian Soil-borne cereal mosaic virus isolates. Parasitica 61:11–16Google Scholar
  48. Ratti C, Rubies-Autonell C, Maccaferri M, Stefanelli S, Sanguineti MC, Vallega V (2006) Reaction of 111 cultivars of Triticum durum Desf. from some of the world’s main genetic pools to soil-borne cereal mosaic virus. J Plant Dis Protect 113:145–149Google Scholar
  49. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  50. Rohlf FJ (1997) NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System Version 2.1. Exeter Software, Setauket, New YorkGoogle Scholar
  51. Rubies-Autonell C, Vallega V (1987) Observations on a mixed Soil-borne wheat mosaic virus and Wheat spindle streak mosaic virus infection in durum wheat (Triticum durum Desf.). J Phytopathol 119:111–121CrossRefGoogle Scholar
  52. Rubies-Autonell C Vallega V (199l) Studies on the development and interaction of Soil-borne wheat mosaic virus and Wheat Spindle streak mosaic virus. In Biotic interactions and soil-borne diseases, Proc. First Congress of the European Foundation of Plant Pathology, Wageningen, Netherlands (Eds. ABR Beemster, GJ Bollen, M Gerlach, MA Ruissen, B.). Schippers and A. Tempel. Elsevier Scientific Publishers, Amsterdam, pp 107–112Google Scholar
  53. Rubies-Autonell C, Vallega V, Ratti C (2003) Reactions of cultivars of common wheat (Triticum aestivum L.) to soilborne wheat mosaic virus in northern Italy during 1996–1997. J Plant Dis Protect 110:332–336Google Scholar
  54. Rubies-Autonell C, Ratti C, Vallega V (2009) Indexed data for comparing the reaction to cereal soil-borne mosaic virus of durum wheat cultivars assayed in different seasons. In: Rush CM (ed) Proc. of the seventh symposium of the international working group on plant viruses with fungal vectors (IWGPVFV). Quedlingburg, GermanyGoogle Scholar
  55. Shaalan M, Heyne EG, Sill WH (1966) Breeding wheat for resistance to soil borne wheat mosaic virus, wheat streak-mosaic virus, leaf rust, stem rust, and bunt. Phytopathology 56:664–669Google Scholar
  56. Shirako Y, Suzuki N, French RC (2000) Similarity and divergence among viruses in the genus Furovirus. Virology 270:201–208PubMedCrossRefGoogle Scholar
  57. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for breeding wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114PubMedCrossRefGoogle Scholar
  58. Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J, Gill BS, Ward R, Cregan PB (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550–560PubMedCrossRefGoogle Scholar
  59. Torrance L, Koenig R (2005) Genus Furovirus. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, London, pp 1027–1032Google Scholar
  60. Vaïanopoulos C, Legrève A, Moreau V, Bragard C (2009) Broad-spectrum detection and quantitation methods of Soil-borne cereal mosaic virus isolates. J Virol Methods 159(2):227–232PubMedCrossRefGoogle Scholar
  61. Vallega V (2004) Soil-borne cereal mosaic. In: Lapierre H, Signoret PA (eds) Viruses, virus diseases of Poaceae (Gramineae). INRA, Paris, pp 612–613Google Scholar
  62. Vallega V, Rubies Autonell C (1985) Reactions of Italian Triticum durum cultivars to soilborne wheat mosaic. Plant Dis 69:64–66CrossRefGoogle Scholar
  63. Vallega V, Rubies-Autonell C, Turina M, Ratti C, Contoli S (1999) Reactions to SBWMV of durum wheat cultivars grown in northern Italy during 1995–96. J Plant Dis Protect 106:284–290Google Scholar
  64. Vallega V, Rubies-Autonell C, Ratti C (2006) Resistance to accumulation of Soil-borne cereal mosaic virus in eight cultivars of Triticum durum Desf. Parasitica 62:79–96Google Scholar
  65. Van Ooijen JW (2006) JoinMap® 4, Software for the calculation of genetic linkage maps in experimental populations. Kyazma BV, WageningenGoogle Scholar
  66. Varshney RK, Tuberosa R (2007) Genomics-assisted crop improvement: an overview. In: Varshney RK, Tuberosa R (eds) Genomics assisted crop improvement, vol. 1: genomics approaches and platforms. Springer, Dordrecht, pp 1–12CrossRefGoogle Scholar
  67. Walker SL, Leath S, Murphy JP, Lommel SA (1998) Selection for resistance and tolerance to oat mosaic virus and oat golden stripe virus in hexaploid oats. Plant Dis 82:423–427CrossRefGoogle Scholar
  68. Weir BS (1996) Genetic data analysis II. Sinauer Associates, SunderlandGoogle Scholar
  69. Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Marco Maccaferri
    • 1
  • Claudio Ratti
    • 1
  • Concepcion Rubies-Autonell
    • 1
  • Victor Vallega
    • 2
  • Andrea Demontis
    • 3
  • Sandra Stefanelli
    • 1
  • Roberto Tuberosa
    • 1
  • Maria Corinna Sanguineti
    • 1
    Email author
  1. 1.Department of Agroenvironmental Science and TechnologyUniversity of BolognaBolognaItaly
  2. 2.Experimental Institute for Cereal ResearchCRARomeItaly
  3. 3.Divisione RicercaSocietà Produttori Sementi Bologna S.p.AArgelato (BO)Italy

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