Theoretical and Applied Genetics

, Volume 123, Issue 1, pp 55–68 | Cite as

Mapping Rph20: a gene conferring adult plant resistance to Puccinia hordei in barley

  • L. T. Hickey
  • W. Lawson
  • G. J. Platz
  • M. Dieters
  • V. N. Arief
  • S. Germán
  • S. Fletcher
  • R. F. Park
  • D. Singh
  • S. Pereyra
  • J. Franckowiak
Original Paper

Abstract

A doubled haploid (DH) barley (Hordeum vulgare L.) population of 334 lines (ND24260 × Flagship) genotyped with DArT markers was used to map genes for adult plant resistance (APR) to leaf rust (Puccinia hordei Otth) under field conditions in Australia and Uruguay. The Australian barley cultivar Flagship carries an APR gene (qRphFlag) derived from the cultivar Vada. Association analysis and composite interval mapping identified two genes conferring APR in this DH population. qRphFlag was mapped to the short arm of chromosome 5H (5HS), accounting for 64–85% of the phenotypic variation across four field environments and 56% under controlled environmental conditions (CEC). A second quantitative trait locus (QTL) from ND24260 (qRphND) with smaller effect was mapped to chromosome 6HL. In the absence of qRphFlag, qRphND conferred only a low level of resistance. DH lines displaying the highest level of APR carried both genes. Sequence information for the critical DArT marker bPb-0837 (positioned at 21.2 cM on chromosome 5HS) was used to develop bPb-0837-PCR, a simple PCR-based marker for qRphFlag. The 245 bp fragment for bPb-0837-PCR was detected in a range of barley cultivars known to possess APR, which was consistent with previous tests of allelism, demonstrating that the qRphFlag resistant allele is common in leaf rust resistant cultivars derived from Vada and Emir. qRphFlag has been designated Rph20, the first gene conferring APR to P. hordei to be characterised in barley. The PCR marker will likely be effective in marker-assisted selection for Rph20.

Supplementary material

122_2011_1566_MOESM1_ESM.doc (94 kb)
Supplementary material 1 (DOC 93.5 kb)

References

  1. Arief VN, Kroonenberg PM, Delacy IH, Dieters MJ, Crossa J, Dreisigacker S, Braun H-J, Basford KE (2010) Construction and three-way ordination of the Wheat Phenome Atlas. J Appl Probab Stat 5:95–118Google Scholar
  2. Butler DG, Cullis BR, Gilmour AR, Gogel BJ (2007) ASReml-R reference manual. Release 2.0. Technical report, Queensland Department of Primary Industries, AustraliaGoogle Scholar
  3. Castro M, Sastre M, Pereyra S, Vázquez D, Ibañez W (2006) Control de enfermedades de cultivares de cebada cervecera en La Estanzuela. Convenio INIA-INASE. http://www.inia.org.uy/convenio_inase_inia/resultados/cebcontrol2006.htm. Accessed 4 November 2010
  4. Clifford BC (1985) Barley leaf rust. In: Roelfs AP, Bushnell WR (eds) The Cereal Rusts. Academic, Orlando, pp 173–205Google Scholar
  5. Cotterill PJ, Rees RG (1993) Evaluation of European slow rusting barleys in Queensland. Trends Agrochem Cultivars 14:166–167Google Scholar
  6. Cotterill PJ, Rees RG, Platz GJ, Dill-Macky R (1992) Effects of leaf rust on selected Australian barleys. Aust J Exp Agr 32:747–751CrossRefGoogle Scholar
  7. Cullis BR, Smith AB, Coombes NE (2006) On the design of early generation variety trials with correlated data. J Agr Biol Env Stat 11:381–393CrossRefGoogle Scholar
  8. Drijepondt SC, Pretorius ZA (1989) Greenhouse evaluation of adult-plant resistance conferred by the gene Lr34 to leaf rust of wheat. Plant Dis 73:669–671CrossRefGoogle Scholar
  9. Drijepondt SC, Pretorius ZA, Rijkenberg FHJ (1991) Expression of two wheat leaf rust resistance gene combinations involving Lr34. Plant Dis 75:526–528CrossRefGoogle Scholar
  10. Dros J (1957) The creation and maintenance of two spring barley varieties. Euphytica 6:45–48CrossRefGoogle Scholar
  11. Gabriel KR (1971) The biplot graphic display of matrices with application to principal component analysis. Biometrika 58:453–467CrossRefGoogle Scholar
  12. Golegaonkar PG (2007) Genetic and molecular analyses of resistance to rust diseases in barley. PhD thesis, The University of Sydney, 147 ppGoogle Scholar
  13. Golegaonkar PG, Park RF, Singh D (2006) Adult plant resistance to Puccinia hordei in barley. In: ProcEedings of International Plant Breeding Symposium, Mexico City, MexicoGoogle Scholar
  14. Golegaonkar PG, Singh D, Park RF (2009) Evaluation of seedling and adult plant resistance to Puccinia hordei in barley. Euphytica 166:183–197CrossRefGoogle Scholar
  15. Golegaonkar PG, Park RF, Singh D (2010) Genetic analysis of adult plant resistance to Puccinia hordei in barley. Plant Breed 129:162–166CrossRefGoogle Scholar
  16. Hickey LH, Dieters MJ, Godwin ID, Kravchuk OY, Aitken EAB, Knight CR, Wilkinson PM, Arief VN, Bansal UK, Bariana HS, DeLacy IH (2011) Rapid phenotyping for adult plant resistance to stripe rust in wheat for high-throughput screening and molecular mapping. Euphytica (Submitted)Google Scholar
  17. Hong M, Singh RP (1996) Contribution of adult plant resistance gene Yr18 in protecting wheat from yellow rust. Plant Dis 80:66–69CrossRefGoogle Scholar
  18. Jacobs Th (1989) Haustorium formation and cell wall appositions in susceptible and partially resistant wheat and barley seedlings infected with wheat leaf rust. J Phytopathol 127:250–261CrossRefGoogle Scholar
  19. Jensen BD, Hockenhull J, Munk L (1999) Seedling and adult plant resistance to downy mildew (Peronospora parasitica) in cauliflower (Brassica oleracea convar. botrytis var. botrytis). Plant Pathol 48:604–612CrossRefGoogle Scholar
  20. Kaur M, Saini RG, Preet K (2000) Adult plant leaf rust resistance from 111 wheat (Triticum aestivum L.) cultivars. Euphytica 113:235–243CrossRefGoogle Scholar
  21. Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1363PubMedCrossRefGoogle Scholar
  22. Lagudah ES, McFadden H, Singh RP, Huerta-Espini J, Bariana HS, Spielmeyer W (2006) Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor Appl Genet 114:21–30PubMedCrossRefGoogle Scholar
  23. Liu F, Gupta S, Zhang X, Jones M, Loughman R, Lance R, Li C (2010) PCR markers for selection of adult plant leaf rust resistance in barley (Hordeum vulgare L.) Mol Breed. doi:10.1007/s11032-010-9517-y
  24. Manly KF, Cudmore RH Jr, Meer JM (2001) Map Manager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932PubMedCrossRefGoogle Scholar
  25. Martínez F, Niks RE, Singh RP, Rubiales D (2001) Characterization of Lr46, a gene conferring partial resistance to wheat leaf rust. Hereditas 135:111–114PubMedCrossRefGoogle Scholar
  26. McIntosh RA (1992) Pre-emptive breeding to control wheat rusts. Euphytica 63:103–113CrossRefGoogle Scholar
  27. McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO Australia, MelbourneGoogle Scholar
  28. McNeal FH, Konzak CF, Smith EP, Tate WS, Russell TS (1971) A uniform system for recording and processing cereal research data. Agricultural Research Service Bulletin 34–121. United States Department of Agriculture, WashingtonGoogle Scholar
  29. Neervoort WJ, Parlevliet JE (1978) Partial resistance of barley to leaf rust, Puccinia hordei. V. Analysis of components of partial resistance in eight barley cultivars. Euphytica 27:33–39CrossRefGoogle Scholar
  30. Niks RE (1986) Failure of haustorial development as a factor in slow growth and development on Puccinia hordei in partially resistant barley seedlings. Physiol Mol Plant Pathol 28:309–322CrossRefGoogle Scholar
  31. Park RF, Karakousis A (2002) Characterization and mapping of gene Rph19 conferring resistance to Puccinia hordei in the cultivar ‘Reka 1’ and several Australian barleys. Plant Breed 121:232–236CrossRefGoogle Scholar
  32. Parlevliet JE (1975) Partial resistance of barley to leaf rust, Puccinia hordei. I. Effect of cultivar and development stage on latent period. Euphytica 24:21–27CrossRefGoogle Scholar
  33. Parlevliet JE (1977) Evidence of differential interaction in the polygenic Hordeum vulgarePuccinia hordei relation during epidemic development. Phytopathology 67:776–778CrossRefGoogle Scholar
  34. Parlevliet JE (1979) Components of resistance that reduce the rate of epidemic development. Annu Rev Phytopathol 17:203–222CrossRefGoogle Scholar
  35. Parlevliet JE (1983) Race specific resistance and cultivar-specific virulence in the barley-leaf rust pathosystem and their consequences for the breeding of leaf rust resistant barley. Euphytica 32:367–375CrossRefGoogle Scholar
  36. Parlevliet JE, van Ommeren A (1975) Partial resistance of barley to leaf rust, Puccinia hordei. II. Relationship between field trials, micro plot tests and latent period. Euphytica 24:293–303CrossRefGoogle Scholar
  37. Patterson HD, Thompson R (1971) Recovery of interblock information when block sizes are unequal. Biometrika 31:100–109Google Scholar
  38. Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can J Res 26:496–500CrossRefGoogle Scholar
  39. Pretorius ZA, Pienaar L, Prins R (2007) Greenhouse and field assessment of adult plant resistance in wheat to Puccinia striiformis f.sp. tritici. Australas Plant Path 36:552–559CrossRefGoogle Scholar
  40. Qi X, Niks RE, Stam P, Lindhout P (1998) Identification of QTLs for partial resistance to leaf rust (Puccinia hordei) in barley. Theor Appl Genet 96:1205–1215CrossRefGoogle Scholar
  41. Qi X, Jiang G, Chen W, Niks RE, Stam P, Lindhout P (1999) Isolate-specific QTLs for partial resistance to Puccinia hordei in barley. Theor Appl Genet 99:877–884CrossRefGoogle Scholar
  42. Rubiales D, Niks RE (1995) Characterization of Lr34, a major gene conferring nonhypersensitive resistance to wheat leaf rust. Plant Dis 79:1208–1212CrossRefGoogle Scholar
  43. Sharp EL, Fuchs E (1982) Additive genes in wheat for resistance to stripe (yellow) rust (Puccinia striiformis Westend.). Crop Prot 1:181–189CrossRefGoogle Scholar
  44. Singh RP, Rajaram S (1994) Genetics of adult-plant resistance to stripe rust in 10 spring bread wheats. Euphytica 72:1–7CrossRefGoogle Scholar
  45. Singh RP, Huerta-Espino J, Rajaram S (2000) Achieving near-immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathol Hun 35:133–139Google Scholar
  46. Stakman EC, Stewart D, Loegering WQ (1962) Identification of physiologic races of Puccinia graminis var. tritici. USDA Agri Res Serv Bull E617:53Google Scholar
  47. Van Os H, Stam P, Visser RGF, Van Eck HJ (2005) RECORD: a novel method for ordering loci on a genetic linkage map. Theor Appl Genet 112:30–40PubMedCrossRefGoogle Scholar
  48. Voorrips RE (2002) MapChart: Software for the graphical presentation of linkage maps and QTL. J Hered 93:77–78PubMedCrossRefGoogle Scholar
  49. Wang S, Basten CJ, Zeng ZB (2007) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC. (http://statgen.ncsu.edu/qtlcart/WQTLCart.htm)
  50. Wenzl P, Li H, Carling J, Zhou M, Raman H, Paul E, Hearnden P, Maier C, Xia L, Caig V, Ovesna J, Cakir M, Poulsen D, Wang J, Raman R, Smith KP, Muehlbauer GJ, Chalmers KJ, Kleinhofs S, Huttner E, Kilian A (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits. BMC Genomics 7:206–228PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • L. T. Hickey
    • 1
  • W. Lawson
    • 2
  • G. J. Platz
    • 2
  • M. Dieters
    • 1
  • V. N. Arief
    • 1
  • S. Germán
    • 3
  • S. Fletcher
    • 4
  • R. F. Park
    • 5
  • D. Singh
    • 5
  • S. Pereyra
    • 3
  • J. Franckowiak
    • 2
  1. 1.The University of Queensland, School of Agriculture and Food SciencesBrisbaneAustralia
  2. 2.Department of EmploymentEconomic Development and Innovation, Hermitage Research StationWarwickAustralia
  3. 3.Instituto Nacional de Investigación Agropecuaria, La EstanzuelaColoniaUruguay
  4. 4.Department of EmploymentEconomic Development and Innovation, Leslie Research Centre ToowoombaToowoombaAustralia
  5. 5.The University of Sydney, Plant Breeding Institute-CobbittyNarellanAustralia

Personalised recommendations