Theoretical and Applied Genetics

, Volume 122, Issue 6, pp 1133–1147 | Cite as

Genetic mapping of DArT markers in the FestucaLolium complex and their use in freezing tolerance association analysis

  • Jan Bartoš
  • Simen Rød Sandve
  • Roland Kölliker
  • David Kopecký
  • Pavla Christelová
  • Štěpán Stočes
  • Liv Østrem
  • Arild Larsen
  • Andrzej Kilian
  • Odd-Arne Rognli
  • Jaroslav Doležel
Original Paper


Species belonging to the FestucaLolium complex are important forage and turf species and as such, have been studied intensively. However, their out-crossing nature and limited availability of molecular markers make genetic studies difficult. Here, we report on saturation of F. pratensis and L. multiflorum genetic maps using Diversity Array Technology (DArT) markers and the DArTFest array.The 530 and 149 DArT markers were placed on genetic maps of L. multiflorum and F. pratensis, respectively, with overlap of 20 markers, which mapped in both species. The markers were sequenced and comparative sequence analysis was performed between L. multiflorum, rice and Brachypodium. The utility of the DArTFest array was then tested on a Festulolium population FuRs0357 in an integrated analysis using the DArT marker map positions to study associations between markers and freezing tolerance. Ninety six markers were significantly associated with freezing tolerance and five of these markers were genetically mapped to chromosomes 2, 4 and 7. Three genomic loci associated with freezing tolerance in the FuRs0357 population co-localized with chromosome segments and QTLs previously indentified to be associated with freezing tolerance. The present work clearly confirms the potential of the DArTFest array in genetic studies of the Festuca–Lolium complex. The annotated DArTFest array resources could accelerate further studies and improvement of desired traits in Festuca–Lolium species.


Freezing Tolerance DArT Marker Forage Grass Brachypodium Syntenic Relationship 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We are grateful to Marie Seifertová, MSc. for excellent technical assistance, and to the team at Diversity Arrays Technology Pty for DArT genotyping. This work has been supported by the Ministry of Agriculture of the Czech Republic (grant award NAZV QH71267) and by European Union (grant No. ED0007/01/01 Centre of the Region Haná for Biotechnological and Agricultural Research).

Supplementary material

122_2010_1518_MOESM1_ESM.xls (118 kb)
Supplementary material 1 (XLS 118 kb)


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Jan Bartoš
    • 1
  • Simen Rød Sandve
    • 2
  • Roland Kölliker
    • 3
  • David Kopecký
    • 1
  • Pavla Christelová
    • 1
  • Štěpán Stočes
    • 1
  • Liv Østrem
    • 4
  • Arild Larsen
    • 5
  • Andrzej Kilian
    • 6
  • Odd-Arne Rognli
    • 2
  • Jaroslav Doležel
    • 1
  1. 1.Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental BotanyOlomoucCzech Republic
  2. 2.Department of Plant and Environmental SciencesNorwegian University of Life SciencesÅsNorway
  3. 3.Agroscope Reckenholz-Tänikon Research Station ARTZurichSwitzerland
  4. 4.Norwegian Institute for Agricultural and Environmental ResearchHellevik i FjalerNorway
  5. 5.Graminor AS, c/o Bioforsk Nord BodøBodøNorway
  6. 6.Diversity Arrays TechnologyYarralumlaAustralia

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