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Theoretical and Applied Genetics

, Volume 122, Issue 3, pp 459–470 | Cite as

Characterization and genetic analysis of a low-temperature-sensitive mutant, sy-2, in Capsicum chinense

  • Song-Ji An
  • Devendra Pandeya
  • Soung-Woo Park
  • Jinjie Li
  • Jin-Kyung Kwon
  • Sota Koeda
  • Munetaka Hosokawa
  • Nam-Chon Paek
  • Doil Choi
  • Byoung-Cheorl Kang
Original Paper

Abstract

A temperature-sensitive mutant of Capsicum chinense, sy-2, shows a normal developmental phenotype when grown above 24°C. However, when grown at 20°C, sy-2 exhibits developmental defects, such as chlorophyll deficiency and shrunken leaves. To understand the underlying mechanism of this temperature-dependent response, phenotypic characterization and genetic analysis were performed. The results revealed abnormal chloroplast structures and cell collapse in leaves of the sy-2 plants grown at 20°C. Moreover, an excessive accumulation of reactive oxygen species (ROS) resulting in cell death was detected in the chlorophyll-deficient sectors of the leaves. However, the expression profile of the ROS scavenging genes did not alter in sy-2 plants grown at 20°C. A further analysis of fatty acid content in the leaves showed the impaired pathway of linoleic acid (18:2) to linolenic acid (18:3). Additionally, the Cafad7 gene was downregulated in sy-2 plants. This change may lead to dramatic physiological disorder and alteration of leaf morphology in sy-2 plants by losing low-temperature tolerance. Genetic analysis of an F2 population from a cross between C. chinensesy-2’ and wild-type C. chinense ‘No. 3341’ showed that the sy-2 phenotype is controlled by a single recessive gene. Molecular mapping revealed that the sy-2 gene is located at a genomic region of the pepper linkage group 1, corresponding to the 300 kb region of the Ch1_scaffold 00106 in tomato chromosome 1. Candidate genes in this region will reveal the identity of sy-2 and the underlying mechanism of the temperature-dependent plant response.

Keywords

Linolenic Acid Chloroplast Development Chloroplast Structure Photooxidative Damage COSII Marker 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was carried out with the support of “the BioGreen21 program (Code20070301034022)”, RDA, and by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

Supplementary material

122_2010_1460_MOESM1_ESM.doc (464 kb)
Supplementary material 1 (DOC 463 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Song-Ji An
    • 1
  • Devendra Pandeya
    • 1
  • Soung-Woo Park
    • 1
  • Jinjie Li
    • 1
    • 2
  • Jin-Kyung Kwon
    • 1
  • Sota Koeda
    • 3
  • Munetaka Hosokawa
    • 3
  • Nam-Chon Paek
    • 1
  • Doil Choi
    • 1
  • Byoung-Cheorl Kang
    • 1
  1. 1.Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life SciencesSeoul National UniversitySeoulKorea
  2. 2.Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture, Beijing Key Lab of Crop Genetic ImprovementChina Agriculture UniversityBeijingChina
  3. 3.Department of Agronomy and Horticultural Science, Graduate School of AgricultureKyoto UniversityKyotoJapan

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