Theoretical and Applied Genetics

, Volume 122, Issue 1, pp 239–249 | Cite as

New slow-rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 in wheat are pleiotropic or closely linked

  • Sybil A. Herrera-Foessel
  • Evans S. Lagudah
  • Julio Huerta-Espino
  • Matthew J. Hayden
  • Harbans S. Bariana
  • Davinder Singh
  • Ravi P. Singh
Original Paper


The common wheat genotype ‘RL6077’ was believed to carry the gene Lr34/Yr18 that confers slow-rusting adult plant resistance (APR) to leaf rust and stripe rust but located to a different chromosome through inter-chromosomal reciprocal translocation. However, haplotyping using the cloned Lr34/Yr18 diagnostic marker and the complete sequencing of the gene indicated Lr34/Yr18 is absent in RL6077. We crossed RL6077 with the susceptible parent ‘Avocet’ and developed F3, F4 and F6 populations from photoperiod-insensitive F3 lines that were segregating for resistance to leaf rust and stripe rust. The populations were characterized for leaf rust resistance at two Mexican sites, Cd. Obregon during the 2008–2009 and 2009–2010 crop seasons, and El Batan during 2009, and for stripe rust resistance at Toluca, a third Mexican site, during 2009. The F3 population was also evaluated for stripe rust resistance at Cobbitty, Australia, during 2009. Most lines had correlated responses to leaf rust and stripe rust, indicating that either the same gene, or closely linked genes, confers resistance to both diseases. Molecular mapping using microsatellites led to the identification of five markers (Xgwm165, Xgwm192, Xcfd71, Xbarc98 and Xcfd23) on chromosome 4DL that are associated with this gene(s), with the closest markers being located at 0.4 cM. In a parallel study in Canada using a Thatcher × RL6077 F3 population, the same leaf rust resistance gene was designated as Lr67 and mapped to the same chromosomal region. The pleiotropic, or closely linked, gene derived from RL6077 that conferred stripe rust resistance in this study was designated as Yr46. The slow-rusting gene(s) Lr67/Yr46 can be utilized in combination with other slow-rusting genes to develop high levels of durable APR to leaf rust and stripe rust in wheat.



We are grateful to Violeta Calvo Salazar, CIMMYT, and Libby Viccars, Sutha Chandramohan, and Lynette Rampling, CSIRO, for their skilled technical support on the molecular studies; to the technical field and greenhouse staff at CIMMYT; to Hanif Miah for the field rust studies at Cobbitty, Australia; to Dr. TR Endo, Japan, for providing the deletion lines used in this study, and to the Grains Research and Development Corporation (GRDC) of Australia for funding the work (Grants CSP00099 and CIM00013). We also thank Alma McNab for the technical editing.

Supplementary material

122_2010_1439_MOESM1_ESM.doc (486 kb)
Fig. S1. Field flag leaf responses to Puccinia triticina (pathotypes MCJ/SP and MBJ/SP) from Mexican site, Ciudad Obregon, in 2010. 1= Avocet S, 2 = Lr34/Yr18 (Yr18/3*Avocet), 3= Lr46/Yr29 (Avocet-YrA*3//LalbMono1*4/Pavon), 4= LrP-7BL (Avocet/Prl), 5= Lr67/Yr46 (Avocet/RL6077), 6= Avocet/Pvn ‘S’ (DOC 287 kb)
122_2010_1439_MOESM2_ESM.doc (1.1 mb)
Fig. S2. Greenhouse flag leaf responses to Puccinia striiformis pathotype Mex08.13.1 = Lr67/Yr46 (Avocet/RL6077), 2 = Lr67/Yr46 (RL6077), 3 = Lr34/Yr18 (RL6058), 4 = Lr46/Yr29 (Avocet-YrA*3//LalbMono1*4/Pavon), 5 = Avocet ‘S’, 6 = Thatcher (DOC 486 kb)
122_2010_1439_MOESM3_ESM.doc (286 kb)
Fig. S3. Thatcher and RL6077 (Thatcher+Lr67/Yr46) probed with an Lr34 cDNA fragment (spans exons 13 to the end of the gene). The 14 lanes shown in the figure corresponds to genomic DNA from the near isogenic pair Thatcher (lanes 1, 3, 5, 7, 9, 11, 13) and RL6077 (lanes 2, 4, 6, 8, 10, 12, 14) cleaved with the restriction endonucleases DraI, EcoRI, EcoRV, HindIII, SacI, XbaI, BglII, NcoI (DOC 1076 kb)


  1. Caldwell RM (1968) Breeding for general and/or specific plant disease resistance. In: Shepherd KW (ed) International wheat genetic symposium, 3rd edn. Academy of Science, CanberraGoogle Scholar
  2. Chen XM, Jones S, Line RF (1995) Chromosomal location of genes for stripe rust resistance in spring wheat cultivars Compair, Fielder, Lee, and Lemhi and interactions of aneuploid wheat with races of Puccinia striiformis. Phytopathology 85:375–381CrossRefGoogle Scholar
  3. Chu CG, Friesen TL, Xu SS, Faris JD, Kolmer JA (2009) Identification of novel QTLs for seedling and adult plant leaf rust resistance in a wheat doubled haploid population. Theor Appl Genet 119:263–269CrossRefPubMedGoogle Scholar
  4. CIMMYT (2005) Laboratory protocols: CIMMYT applied molecular genetics laboratory, 3rd edn. CIMMYT, MexicoGoogle Scholar
  5. Dyck PL (1977) Genetics of leaf rust resistance in three introductions of common wheat. Can J Genet Cytol 19:711–716Google Scholar
  6. Dyck PL (1987) The association of a gene for leaf rust resistance with the chromosome 7D suppressor of stem rust resistance in common wheat. Genome 29:467–469Google Scholar
  7. Dyck PL, Samborski DJ (1979) Adult-plant leaf rust resistance in PI250413, and introduction of common wheat. Can J Plant Sci 59:329–332CrossRefGoogle Scholar
  8. Dyck PL, Samborski DJ (1982) The inheritance of resistance to Puccinia recondita in a group of common wheat cultivars. Can J Genet Cytol 24:273–283Google Scholar
  9. Dyck PL, Kerber ER, Aung T (1994) An interchromosomal reciprocal translocation in wheat involving leaf rust resistance gene Lr34. Genome 37:556–559CrossRefPubMedGoogle Scholar
  10. Gavin Vanegas CD, Garvin DF, Kolmer JA (2008) Genetics of stem rust resistance in the spring wheat cultivar Thatcher and the enhancement of stem rust resistance by Lr34. Euphytica 159:391–401CrossRefGoogle Scholar
  11. Hayden MJ, Nguyen TM, Waterman A, McMichael GL, Chalmers KJ (2008) Application of multiplex-ready PCR for fluorescence-based SSR genotyping in barley and wheat. Mol Breeding 21:271–281CrossRefGoogle Scholar
  12. Hiebert CW, Thomas JB, McCallum BD, Humphreys DG, DePauw RM, Hayden MJ, Mago R, Schnippenkoetter W, Spielmeyer W (2010) An introgression on wheat chromosome 4DL in RL6077 (Thatcher*6/PI 250413) confers adult plant resistance to stripe rust and leaf rust (Lr67). Theor Appl Genet. doi: 10.1007/s00122-010-1373-y
  13. Kolmer JA, Singh RP, Garvin DF, Viccars L, William HM, Huerta-Espino J, Ogbonnaya FC, Raman H, Orford S, Bariana HS, Lagudah ES (2008) Analysis of the Lr34/Yr18 rust resistance region in wheat germplasm. Crop Sci 48:1841–1852CrossRefGoogle Scholar
  14. Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1363CrossRefPubMedGoogle Scholar
  15. Lagudah ES, Appels R, McNeil D (1991a) The Nor-D3 locus of Triticum tauschii: natural variation and linkage to chromosome 5 markers. Genome 34:387–395Google Scholar
  16. Lagudah ES, Appels R, Brown AHD, McNeil D (1991b) The molecular-genetic analysis of Triticum tauschii—the D genome donor to hexaploid wheat. Genome 34:375–386Google Scholar
  17. Lagudah ES, Krattinger SG, Herrera-Foessel S, Singh RP, Huerta-Espino J, Spielmeyer W, Brown-Guedira G, Selter LL, Keller B (2009) Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theor Appl Genet 119:889–898CrossRefPubMedGoogle Scholar
  18. Lander E, Green P, Abrahamson J, Barlow A, Daley M, Lincoln S, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181CrossRefPubMedGoogle Scholar
  19. Lillemo M, Asalf B, Singh RP, Huerta-Espino J, Chen XM, He ZH, Bjørnstad Å (2008) The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theor Appl Genet 116:1155–1166CrossRefPubMedGoogle Scholar
  20. McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Somers DJ, Appels R, Devos KM (2008) Catalogue of gene symbols for wheat.
  21. McNeal FH, Konzak CF, Smith EP, Tate WS, Russell TS (1971) A uniform system for recording and processing cereal research data. USDA-ARS Bull, Washington, pp 34–121Google Scholar
  22. Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can J Res 26:496–500Google Scholar
  23. Roelfs AP, Singh RP, Saari EE (1992) Rust diseases of wheat: concepts and methods of disease management. CIMMYT, MexicoGoogle Scholar
  24. Shang HS, Dyck PL, Samborski DJ (1986) Inheritance of resistance to Puccinia recondita in a group of resistant accessions of common wheat. Can J Plant Pathol 8:123–131CrossRefGoogle Scholar
  25. Singh RP (1992a) Genetic association of leaf rust resistance gene Lr34 with adult plant resistance to stripe rust in bread wheat. Phytopathology 82:835–838CrossRefGoogle Scholar
  26. Singh RP (1992b) Association between gene Lr34 for leaf rust resistance and leaf tip necrosis in wheat. Crop Sci 32:874–878CrossRefGoogle Scholar
  27. Singh RP (1993) Genetic association of gene Bydv1 for tolerance to barley yellow dwarf virus with genes Lr34 and Yr18 for adult plant resistant to rusts in bread wheat. Plant Dis 77:1103–1106CrossRefGoogle Scholar
  28. Singh RP, Huerta-Espino J, Rajaram S (2000a) Achieving near-immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathologica et Entomologica Hungarica 35:133–139Google Scholar
  29. Singh RP, Nelson JC, Sorrells ME (2000b) Mapping Yr28 and other genes for resistance to stripe rust in wheat. Crop Sci 40:1148–1155CrossRefGoogle Scholar
  30. Singh RP, Huerta-Espino J, William HM (2005) Genetics and breeding for durable resistance to leaf and stripe rusts in wheat. Turk J Agric For 29:121–127Google Scholar
  31. Spielmeyer W, McIntosh RA, Kolmer J, Lagudah ES (2005) Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust cosegregate at a locus on the short arm of chromosome 7D of wheat. Theor Appl Genet 111:731–735CrossRefPubMedGoogle Scholar
  32. Suenaga K, Singh RP, Huerta-Espino J, William HM (2003) Microsatellite markers for genes Lr34/Yr18 and other quantitative trait loci for leaf rust and stripe rust resistance in bread wheat. Phytopathology 93:881–890CrossRefPubMedGoogle Scholar
  33. Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Sybil A. Herrera-Foessel
    • 1
  • Evans S. Lagudah
    • 2
  • Julio Huerta-Espino
    • 3
  • Matthew J. Hayden
    • 4
  • Harbans S. Bariana
    • 5
  • Davinder Singh
    • 6
  • Ravi P. Singh
    • 1
  1. 1.International Maize and Wheat Improvement Center (CIMMYT)Mexico, D.F.Mexico
  2. 2.CSIRO Plant IndustryCanberraAustralia
  3. 3.Campo Experimental Valle de México INIFAPChapingoMexico
  4. 4.Department of Primary IndustriesVictorian AgriBiosciences CenterBundooraAustralia
  5. 5.The University of Sydney Plant Breeding Institute-CobbittyNarellanAustralia
  6. 6.CIMMYT, ICRAF HouseNairobiKenya

Personalised recommendations