Theoretical and Applied Genetics

, Volume 122, Issue 1, pp 239–249 | Cite as

New slow-rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 in wheat are pleiotropic or closely linked

  • Sybil A. Herrera-Foessel
  • Evans S. Lagudah
  • Julio Huerta-Espino
  • Matthew J. Hayden
  • Harbans S. Bariana
  • Davinder Singh
  • Ravi P. Singh
Original Paper

Abstract

The common wheat genotype ‘RL6077’ was believed to carry the gene Lr34/Yr18 that confers slow-rusting adult plant resistance (APR) to leaf rust and stripe rust but located to a different chromosome through inter-chromosomal reciprocal translocation. However, haplotyping using the cloned Lr34/Yr18 diagnostic marker and the complete sequencing of the gene indicated Lr34/Yr18 is absent in RL6077. We crossed RL6077 with the susceptible parent ‘Avocet’ and developed F3, F4 and F6 populations from photoperiod-insensitive F3 lines that were segregating for resistance to leaf rust and stripe rust. The populations were characterized for leaf rust resistance at two Mexican sites, Cd. Obregon during the 2008–2009 and 2009–2010 crop seasons, and El Batan during 2009, and for stripe rust resistance at Toluca, a third Mexican site, during 2009. The F3 population was also evaluated for stripe rust resistance at Cobbitty, Australia, during 2009. Most lines had correlated responses to leaf rust and stripe rust, indicating that either the same gene, or closely linked genes, confers resistance to both diseases. Molecular mapping using microsatellites led to the identification of five markers (Xgwm165, Xgwm192, Xcfd71, Xbarc98 and Xcfd23) on chromosome 4DL that are associated with this gene(s), with the closest markers being located at 0.4 cM. In a parallel study in Canada using a Thatcher × RL6077 F3 population, the same leaf rust resistance gene was designated as Lr67 and mapped to the same chromosomal region. The pleiotropic, or closely linked, gene derived from RL6077 that conferred stripe rust resistance in this study was designated as Yr46. The slow-rusting gene(s) Lr67/Yr46 can be utilized in combination with other slow-rusting genes to develop high levels of durable APR to leaf rust and stripe rust in wheat.

Notes

Acknowledgments

We are grateful to Violeta Calvo Salazar, CIMMYT, and Libby Viccars, Sutha Chandramohan, and Lynette Rampling, CSIRO, for their skilled technical support on the molecular studies; to the technical field and greenhouse staff at CIMMYT; to Hanif Miah for the field rust studies at Cobbitty, Australia; to Dr. TR Endo, Japan, for providing the deletion lines used in this study, and to the Grains Research and Development Corporation (GRDC) of Australia for funding the work (Grants CSP00099 and CIM00013). We also thank Alma McNab for the technical editing.

Supplementary material

122_2010_1439_MOESM1_ESM.doc (486 kb)
Fig. S1. Field flag leaf responses to Puccinia triticina (pathotypes MCJ/SP and MBJ/SP) from Mexican site, Ciudad Obregon, in 2010. 1= Avocet S, 2 = Lr34/Yr18 (Yr18/3*Avocet), 3= Lr46/Yr29 (Avocet-YrA*3//LalbMono1*4/Pavon), 4= LrP-7BL (Avocet/Prl), 5= Lr67/Yr46 (Avocet/RL6077), 6= Avocet/Pvn ‘S’ (DOC 287 kb)
122_2010_1439_MOESM2_ESM.doc (1.1 mb)
Fig. S2. Greenhouse flag leaf responses to Puccinia striiformis pathotype Mex08.13.1 = Lr67/Yr46 (Avocet/RL6077), 2 = Lr67/Yr46 (RL6077), 3 = Lr34/Yr18 (RL6058), 4 = Lr46/Yr29 (Avocet-YrA*3//LalbMono1*4/Pavon), 5 = Avocet ‘S’, 6 = Thatcher (DOC 486 kb)
122_2010_1439_MOESM3_ESM.doc (286 kb)
Fig. S3. Thatcher and RL6077 (Thatcher+Lr67/Yr46) probed with an Lr34 cDNA fragment (spans exons 13 to the end of the gene). The 14 lanes shown in the figure corresponds to genomic DNA from the near isogenic pair Thatcher (lanes 1, 3, 5, 7, 9, 11, 13) and RL6077 (lanes 2, 4, 6, 8, 10, 12, 14) cleaved with the restriction endonucleases DraI, EcoRI, EcoRV, HindIII, SacI, XbaI, BglII, NcoI (DOC 1076 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Sybil A. Herrera-Foessel
    • 1
  • Evans S. Lagudah
    • 2
  • Julio Huerta-Espino
    • 3
  • Matthew J. Hayden
    • 4
  • Harbans S. Bariana
    • 5
  • Davinder Singh
    • 6
  • Ravi P. Singh
    • 1
  1. 1.International Maize and Wheat Improvement Center (CIMMYT)Mexico, D.F.Mexico
  2. 2.CSIRO Plant IndustryCanberraAustralia
  3. 3.Campo Experimental Valle de México INIFAPChapingoMexico
  4. 4.Department of Primary IndustriesVictorian AgriBiosciences CenterBundooraAustralia
  5. 5.The University of Sydney Plant Breeding Institute-CobbittyNarellanAustralia
  6. 6.CIMMYT, ICRAF HouseNairobiKenya

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