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Theoretical and Applied Genetics

, Volume 121, Issue 6, pp 1083–1091 | Cite as

An introgression on wheat chromosome 4DL in RL6077 (Thatcher*6/PI 250413) confers adult plant resistance to stripe rust and leaf rust (Lr67)

  • Colin W. Hiebert
  • Julian B. Thomas
  • Brent D. McCallum
  • D. Gavin Humphreys
  • Ronald M. DePauw
  • Matthew J. Hayden
  • Rohit Mago
  • Wendelin Schnippenkoetter
  • Wolfgang Spielmeyer
Original Paper

Abstract

Adult plant resistance (APR) to leaf rust and stripe rust derived from the wheat (Triticum aestivum L.) line PI250413 was previously identified in RL6077 (=Thatcher*6/PI250413). The leaf rust resistance gene in RL6077 is phenotypically similar to Lr34 which is located on chromosome 7D. It was previously hypothesized that the gene in RL6077 could be Lr34 translocated to another chromosome. Hybrids between RL6077 and Thatcher and between RL6077 and 7DS and 7DL ditelocentric stocks were examined for first meiotic metaphase pairing. RL6077 formed chain quadrivalents and trivalents relative to Thatcher and Chinese Spring; however both 7D telocentrics paired only as heteromorphic bivalents and never with the multivalents. Thus, chromosome 7D is not involved in any translocation carried by RL6077. A genome-wide scan of SSR markers detected an introgression from chromosome 4D of PI250413 transferred to RL6077 through five cycles of backcrossing to Thatcher. Haplotype analysis of lines from crosses of Thatcher × RL6077 and RL6058 (Thatcher*6/PI58548) × RL6077 showed highly significant associations between introgressed markers (including SSR marker cfd71) and leaf rust resistance. In a separate RL6077-derived population, APR to stripe rust was also tightly linked with cfd71 on chromosome 4DL. An allele survey of linked SSR markers cfd71 and cfd23 on a set of 247 wheat lines from diverse origins indicated that these markers can be used to select for the donor segment in most wheat backgrounds. Comparison of RL6077 with Thatcher in field trials showed no effect of the APR gene on important agronomic or quality traits. Since no other known Lr genes exist on chromosome 4DL, the APR gene in RL6077 has been assigned the name Lr67.

Keywords

Leaf Rust Rust Resistance Stripe Rust Pollen Mother Cell Adult Plant Resistance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We acknowledge the excellent technical assistance of Pat Seto-Goh, Mira Popovic, Erica Riedel and Denis Green in Canada and Jessica Hyles and Keshab Kandel in Australia. We also thank the Grains Research and Development Corportion (GRDC), Australia for financial support.

Supplementary material

122_2010_1373_MOESM1_ESM.doc (236 kb)
Supplementary Table S1 (DOC 236 kb)

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Copyright information

© Her Majesty the Queen in Rights of Canada 2010

Authors and Affiliations

  • Colin W. Hiebert
    • 1
  • Julian B. Thomas
    • 1
  • Brent D. McCallum
    • 1
  • D. Gavin Humphreys
    • 1
  • Ronald M. DePauw
    • 2
  • Matthew J. Hayden
    • 3
  • Rohit Mago
    • 4
  • Wendelin Schnippenkoetter
    • 4
  • Wolfgang Spielmeyer
    • 4
  1. 1.Cereal Research Centre, Agriculture and Agri-Food CanadaWinnipegCanada
  2. 2.Semiarid Prairie Agricultural Research Centre, Agriculture and Agri-Food CanadaSwift CurrentCanada
  3. 3.Department of Primary IndustriesVictorian AgriBioscience Centre, La Trobe Research and Development ParkBundooraAustralia
  4. 4.CSIRO Plant IndustryCanberraAustralia

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