Theoretical and Applied Genetics

, Volume 121, Issue 1, pp 127–136 | Cite as

Pyramiding QTL increases seedling resistance to crown rot (Fusarium pseudograminearum) of wheat (Triticum aestivum)

  • W. D. Bovill
  • M. Horne
  • D. Herde
  • M. Davis
  • G. B. Wildermuth
  • M. W. SutherlandEmail author
Original Paper


Crown rot of wheat (Triticum aestivum), predominantly caused by the fungus Fusarium pseudograminearum, has become an increasingly important disease constraint in many winter cereal production regions in Australia. Our group has previously identified a range of quantitative trait loci (QTL) for partial resistance to crown rot in various bread wheat sources. Here, we report on work that has assessed the effectiveness of pyramiding QTL to improve resistance to crown rot. Two doubled haploid populations were analysed—one from a cross between two previously characterised sources of partial seedling resistance (2-49 and W21MMT70; n = 208) and one from a cross between 2-49 and the commercial variety Sunco, a source of adult field resistance (n = 134). Both populations were phenotyped for seedling resistance to crown rot. Microsatellite and DArT markers were used to construct whole genome linkage maps for use in composite interval mapping (CIM) to identify QTL. Three QTL were detected in both trials conducted on the 2-49/W21MMT70 population. These were located on chromosomes 1D (QCr.usq-1D.1), 3B (QCr.usq-3B.1) and 7A. QCr.usq-1D.1 and the previously undetected 7A QTL were inherited from 2-49. QCr.usq-3B.1, inherited from W21MMT70, was the most significant of the QTL, explaining up to 40.5% of the phenotypic variance. Three QTL were identified in multiple trials of the Sunco/2-49 population. These were located on chromosomes 1D (QCr.usq-1D.1), 2B (QCr.usq-2B.2) and 4B (QCr.usq-4B.1). Only QCr.usq-2B.2 was inherited from Sunco. QCr.usq-4B.1 was the most significant of these QTL, explaining up to 19.1% of the phenotypic variance. In the 2-49/W21MMT70 population, several DH lines performed significantly better than either parent, with the best recording an average disease severity rating of only 3.8% of that scored by the susceptible check cultivar Puseas. These lines represent a new level of seedling crown rot resistance in wheat.


Quantitative Trait Locus Quantitative Trait Locus Mapping DArT Marker Quantitative Trait Locus Detection Resistance Quantitative Trait Locus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors would like to acknowledge Sally Coverdale, Maria Harris, Boyd McNamara and Tina Walters for assistance with phenotyping, and our USQ colleague Dr. Anke Lehmensiek for commenting on the manuscript. This research was funded by the Grains Research and Development Corporation, project USQ 00007.


  1. Backhouse D, Abubakar AA, Burgess LW, Dennis JI, Hollaway GJ, Wildermuth GB, Wallwork H, Henry FJ (2004) Survey of Fusarium species associated with crown rot of wheat and barley in eastern Australia. Australas Plant Pathol 33:255–261CrossRefGoogle Scholar
  2. Beavis WD (1994) The power and deceit of QTL experiments: lessons from comparative QTL studies. In: Proceedings of the Forty-ninth Sorghum Industry Research Conference. American Seed Trade Association, Washington, DCGoogle Scholar
  3. Bovill WD, Ma W, Ritter K, Collard BCY, Davis M, Wildermuth GB, Sutherland MW (2006) Identification of novel QTL for resistance to crown rot in the doubled haploid wheat population ‘W21MMT70’ × ‘Mendos’. Plant Breed 125:538–543CrossRefGoogle Scholar
  4. Brennan JP, Murray GM (2009) Current and potential costs from diseases of wheat in Australia. GRDC Report. ISBN 978-1-875477-92-0Google Scholar
  5. Carlborg O, Haley CS (2004) Epistasis: too often neglected in complex trait studies? Nat Rev Genet 5:618–625CrossRefPubMedGoogle Scholar
  6. Cheverud JM, Routman EJ (1995) Epistasis and its contribution to genetic variance components. Genetics 139:1455–1461PubMedGoogle Scholar
  7. Collard BCY, Grams RA, Bovill WD, Percy CD, Jolley R, Lehmensiek A, Wildermuth GB, Sutherland MW (2005) Development of molecular markers for crown rot resistance in wheat: mapping of QTLs for seedling resistance in a 2–49 x Janz population. Plant Breed 124:1–6CrossRefGoogle Scholar
  8. Collard BCY, Jolley R, Bovill WD, Grams RA, Wildermuth GB, Sutherland MW (2006) Confirmation of QTL mapping and marker validation for partial seedling resistance to crown rot in wheat line ‘2-49’. Aust J Agric Res 57:967–973CrossRefGoogle Scholar
  9. Dekkers JCM, Hospital F (2002) The use of molecular genetics in the improvement of agricultural populations. Nat Rev Genet 3:22–32CrossRefPubMedGoogle Scholar
  10. Dodman RL, Wildermuth GB, Cameron HF, Lavers JRW (1980) Plant pathology and soil microbiology: research on fungal diseases. Biennial report 1978–1980. Queensland Department of Primary Industries, ToowoombaGoogle Scholar
  11. Kammholz SJ, Campbell AW, Sutherland MW, Hollamby GJ, Martin PJ, Eastwood RF, Barclay I, Wilson RE, Sheppard JA (2001) Establishment and characterisation of wheat genetic mapping populations. Aust J Agric Res 52:1079–1088CrossRefGoogle Scholar
  12. Kearsey MJ, Pooni HS (1996) The genetical analysis of quantitative traits. Stanley Thornes, BirminghamGoogle Scholar
  13. Klein TA, Burgess LW, Ellison FW (1991) The incidence and spatial patterns of wheat plants infected by Fusarium graminearum Group 1 and the effect of crown rot on yield. Aust J Agric Res 42:399–407CrossRefGoogle Scholar
  14. Lehmensiek A, Eckermann PJ, Verbyla AP, Appels R, Sutherland MW, Daggard GE (2005) Curation of wheat maps to improve map accuracy and QTL detection. Aust J Agric Res 56:1347–1354CrossRefGoogle Scholar
  15. Li X, Liu C, Chakraborty S, Manners JM, Kazan K (2008) A simple method for the assessment of crown rot disease severity in wheat seedlings inoculated with Fusarium pseudograminearum. J Phytopath 156:751–754CrossRefGoogle Scholar
  16. Manly KF, Cudmore RHJ, Meer JM (2001) Map Manager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932CrossRefPubMedGoogle Scholar
  17. Miedaner T, Wilde F, Steiner B, Buerstmayr H, Korzun V, Ebmeyer E (2006) Stacking quantitative trait loci (QTL) for Fusarium head blight resistance from non-adapted sources in an European elite spring wheat background and assessing their effect of deoxynivalenol (DON) content and disease severity. Theor Appl Genet 112:562–569CrossRefPubMedGoogle Scholar
  18. Mitter V, Zhang MC, Liu CJ, Ghosh R, Ghosh M, Chakraborty S (2006) A high-throughput glasshouse bioassay to detect crown rot resistance in wheat germplasm. Plant Pathol 55:433–442CrossRefGoogle Scholar
  19. Smiley RW, Gourlie JA, Easley SA, Patterson LM, Whittaker RG (2005) Crop damage estimates for crown rot of wheat and barley in the Pacific Northwest. Plant Dis 89:595–604CrossRefGoogle Scholar
  20. Van Os H, Stam P, Visser RGF, Van Eck HJ (2005) RECORD: a novel method for ordering loci on a genetic linkage map. Theor Appl Genet 112:30–40CrossRefPubMedGoogle Scholar
  21. Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTL. Heredity 93:77–78CrossRefGoogle Scholar
  22. Wade MJ, Winther RG, Agrawal A, Goodnight CJ (2001) Alternative definitions of epistasis: dependence and interaction. Trends Ecol Evolut 16:498–504CrossRefGoogle Scholar
  23. Wallwork H, Butt M, Cheong JPE, Williams KJ (2004) Resistance to crown rot in wheat identified through an improved method for screening adult plants. Australas Plant Pathol 33:1–7CrossRefGoogle Scholar
  24. Wang S, Basten CJ, Zeng Z-B (2007) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC. (
  25. Wildermuth GB, McNamara RB (1994) Testing wheat seedlings for resistance to crown rot caused by Fusarium graminearum Group 1. Plant Dis 78:949–953Google Scholar
  26. Wildermuth GB, Morgan JM (2004) Genotypic differences in partial resistance to crown rot caused by Fusarium pseudograminearum in relation to an osmoregulation gene in wheat. Australas Plant Pathol 33:121–123CrossRefGoogle Scholar
  27. Wildermuth GB, McNamara RB, Quick JM (2001) Crown depth and susceptibility to crown rot in wheat. Euphytica 122:397–405CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • W. D. Bovill
    • 1
    • 3
  • M. Horne
    • 1
  • D. Herde
    • 2
  • M. Davis
    • 2
  • G. B. Wildermuth
    • 2
  • M. W. Sutherland
    • 1
    Email author
  1. 1.Faculty of Sciences, Centre for Systems BiologyUniversity of Southern QueenslandToowoombaAustralia
  2. 2.Department of Employment, Economic Development and InnovationLeslie Research CentreToowoombaAustralia
  3. 3.School of Agriculture, Food and WineUniversity of AdelaideGlen OsmondAustralia

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