Theoretical and Applied Genetics

, Volume 120, Issue 8, pp 1525–1534 | Cite as

Effects of ascertainment bias and marker number on estimations of barley diversity from high-throughput SNP genotype data

  • M. Moragues
  • J. Comadran
  • R. Waugh
  • I. Milne
  • A. J. Flavell
  • Joanne R. RussellEmail author
Original Paper


The capability of molecular markers to provide information of genetic structure is influenced by their number and the way they are chosen. This study evaluates the effects of single nucleotide polymorphism (SNP) number and selection strategy on estimates of germplasm diversity and population structure for different types of barley germplasm, namely cultivar and landrace. One hundred and sixty-nine barley landraces from Syria and Jordan and 171 European barley cultivars were genotyped with 1536 SNPs. Different subsets of 384 and 96 SNPs were selected from the 1536 set, based on their ability to detect diversity in landraces or cultivated barley in addition to corresponding randomly chosen subsets. All SNP sets except the landrace-optimised subsets underestimated the diversity present in the landrace germplasm, and all subsets of SNP gave similar estimates for cultivar germplasm. All marker subsets gave qualitatively similar estimates of the population structure in both germplasm sets, but the 96 SNP sets showed much lower data resolution values than the larger SNP sets. From these data we deduce that pre-selecting markers for their diversity in a germplasm set is very worthwhile in terms of the quality of data obtained. Second, we suggest that a properly chosen 384 SNP subset gives a good combination of power and economy for germplasm characterization, whereas the rather modest gain from using 1536 SNPs does not justify the increased cost and 96 markers give unacceptably low performance. Lastly, we propose a specific 384 SNP subset as a standard genotyping tool for middle-eastern landrace barley.


Single Nucleotide Polymorphism Single Nucleotide Polymorphism Marker Ascertainment Bias Wild Barley Single Nucleotide Polymorphism Genotyping 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We would like to acknowledge Drs S. Grando, M. Baum and S. Ceccarelli at International Center for Agricultural Research in the Dry Areas (ICARDA) for providing the Syrian Jordanian landrace collection material. The above work was supported by BBSRC Grant BB/E024726/1 (EXBARDIV) under the ERA-PG Programme ‘Structuring Plant Genomic Research in Europe’. SCRI received Grant-in-Aid from the Scottish Government.

Supplementary material

122_2010_1273_MOESM1_ESM.doc (174 kb)
Supplementary Table 1 and Figure 1 (DOC 174 kb)


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • M. Moragues
    • 1
  • J. Comadran
    • 2
  • R. Waugh
    • 2
  • I. Milne
    • 2
  • A. J. Flavell
    • 1
  • Joanne R. Russell
    • 2
    Email author
  1. 1.Division of Plant SciencesUniversity of Dundee at SCRIDundeeUK
  2. 2.Genetics Programme, Scottish Crop Research InstituteDundeeScotland, UK

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