Theoretical and Applied Genetics

, Volume 120, Issue 7, pp 1279–1287 | Cite as

Molecular mapping and candidate gene identification of the Rf2 gene for pollen fertility restoration in sorghum [Sorghum bicolor (L.) Moench]

  • D. R. Jordan
  • Emma S. Mace
  • R. G. Henzell
  • P. E. Klein
  • R. R. Klein
Original Paper


The A1 cytoplasmic–nuclear male sterility system in sorghum is used almost exclusively for the production of commercial hybrid seed and thus, the dominant genes that restore male fertility in F1 hybrids are of critical importance to commercial seed production. The genetics of fertility restoration in sorghum can appear complex, being controlled by at least two major genes with additional modifiers and additional gene–environment interaction. To elucidate the molecular processes controlling fertility restoration and to develop a marker screening system for this important trait, two sorghum recombinant inbred line populations were created by crossing a restorer and a non-restoring inbred line, with fertility phenotypes evaluated in hybrid combination with three unique cytoplasmic male sterile lines. In both populations, a single major gene segregated for restoration which was localized to chromosome SBI-02 at approximately 0.5 cM from microsatellite marker, Xtxp304. In the two populations we observed that approximately 85 and 87% of the phenotypic variation in seed set was associated with the major Rf gene on SBI-02. Some evidence for modifier genes was also observed since a continuum of partial restored fertility was exhibited by lines in both RIL populations. With the prior report (Klein et al. in Theor Appl Genet 111:994–1012, 2005) of the cloning of the major fertility restoration gene Rf1 in sorghum, the major fertility restorer locus identified in this study was designated Rf2. A fine-mapping population was used to resolve the Rf2 locus to a 236,219-bp region of chromosome SBI-02, which spanned ~31 predicted open reading frames including a pentatricopeptide repeat (PPR) gene family member. The PPR gene displayed high homology with rice Rf1. Progress towards the development of a marker-assisted screen for fertility restoration is discussed.


Sorghum Recombinant Inbred Line Recombinant Inbred Line Population Fertility Restoration Restorer Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank the Australian Grains Research and Development Cooperation (GRDC;, USDA-ARS and Queensland Primary Industries and Fisheries providing financial support for this research.

Supplementary material

122_2009_1255_MOESM1_ESM.doc (44 kb)
Supplementary Table 1 (DOC 44 kb)


  1. Ahnert D, Lee M, Austin DF, Livini C, Woodman WL, Openshaw SJ, Smith JSC, Porter K, Dalton G (1996) Genetic diversity among elite sorghum inbred lines assessed with DNA markers and pedigree information. Crop Sci 36:1385–1392Google Scholar
  2. Bhattramakki D, Dong J, Chhabra AK, Hart GE (2000) An integrated SSR and RFLP linkage map of Sorghum bicolor (L.) Moench. Genome 43:988–1002CrossRefPubMedGoogle Scholar
  3. Brooking IR (1976) Male sterility in Sorghum bicolor (L.) Moench induced by low night temperature. I. Timing of the stage of sensitivity. Aust J Plant Physiol 3:589–596CrossRefGoogle Scholar
  4. Brooking IR (1979) Male sterility in Sorghum bicolor (L.) Moench induced by low night temperature. II. Genotypic differences in sensitivity. Aust J Plant Physiol 6:143–147CrossRefGoogle Scholar
  5. Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971Google Scholar
  6. Doggett H (1988) Sorghum, 2nd edn. Longman Scientific and Technical co-published with John Wiley and Sons, New YorkGoogle Scholar
  7. Downs RW, Marshall DR (1971) Low temperature induced male sterility in grain sorghum. Aust J Agric Res Animal Husb 11:352–356CrossRefGoogle Scholar
  8. Erichsen AW, Ross JG (1963) Irregularities at microsporogenesis in colchicine-induced male-sterile mutants in Sorghum vulgare Pers. Crop Sci 3:481–483CrossRefGoogle Scholar
  9. Klein RR, Klein PE, Chhabra AK, Dong J, Pammi S, Childs KL, Mullet JE, Rooney WL, Schertz KF (2001) Molecular mapping of the Rf1 gene for pollen fertility restoration in sorghum (Sorghum bicolor L.). Theor Appl Genet 102:1206–1212CrossRefGoogle Scholar
  10. Klein RR, Klein PE, Mullet JE, Minx P, Rooney WL, Schertz KF (2005) Fertility restorer locus Rf1 of sorghum (Sorghum bicolor L.) encodes a pentatricopeptide repeat protein not present in the colinear region of rice chromosome 12. Theor Appl Genet 111:994–1012CrossRefPubMedGoogle Scholar
  11. Lurin C, Andres C, Aubourg S, Bellaoui M, Bitton F, Bruyere C, Caboche M, Debast C, Gualberto J, Hoffmann B, Lecharny A, Le Ret M, Martin-Magniette ML, Mireau H, Peeters N, Renou JP, Szurek B, Taconnat L, Small I (2004) Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins reveals their essential role in organelle biogenesis. Plant Cell 16:2089–2103CrossRefPubMedGoogle Scholar
  12. Mace ES, Xia L, Jordan DR, Halloran K, Parh DK, Huttner E, Wenzl P, Kilian A (2008) DArT markers: diversity analyses and mapping in Sorghum bicolor. BMC Genomics 9:26CrossRefPubMedGoogle Scholar
  13. Mace ES, Rami J-F, Bouchet S, Klein PE, Klein RR, Kilian A, Wenzl P, Xia L, Sakrewski K, Jordan DR (2009) A consensus genetic map of sorghum that integrates multiple component maps and high-throughput Diversity Array Technology (DArT) markers. BMC Plant Biol 9:13CrossRefPubMedGoogle Scholar
  14. Maunder B, Pickett RC (1959) The genetic inheritance of cytoplasmic-genetic male sterility in grain sorghum. Agron J 51:47–49Google Scholar
  15. Menz MA, Klein RR, Unruh NC, Rooney WL, Klein PE, Mullet JE (2004) Genetic diversity of public inbreds of sorghum determined by mapped AFLP and SSR markers. Crop Sci 44:1236–1244CrossRefGoogle Scholar
  16. Mester D, Ronin Y, Minkov D, Nevo E, Korol A (2003) Constructing large-scale genetic maps using an evolutionary strategy algorithm. Genetics 165:2269–2282PubMedGoogle Scholar
  17. Miller DA, Pickett RC (1964) Inheritance of partial male-fertility in Sorghum vulgare Pers. Crop Sci 4:1–4CrossRefGoogle Scholar
  18. Parh D, Jordan DR, Aitken E, Mace ES, Junai P, McIntyre C, Godwin I (2008) QTL analysis of ergot resistance in sorghum. Theor Appl Genet 117:369–382CrossRefPubMedGoogle Scholar
  19. Saha D, Prasad AM, Srinivasan (2007) Pentatricopeptide repeat proteins and their emerging role in plants. Plant Physiol Biochem 45:521–534CrossRefPubMedGoogle Scholar
  20. Schertz KF, Sotomayor-Rios A, Torres-Cardona S (1989) Cytoplasmic-nuclear male sterility: opportunities in breeding and genetics. Proc Grain Sorghum Res and Utility Conf 16:175–186Google Scholar
  21. Stephens JC, Holland RF (1954) Cytoplasmic male sterility for hybrid sorghum seed production. Agron J 46:20–23CrossRefGoogle Scholar
  22. Stephens JC, Miller FR, Rosenow DT (1967) Conversion of alien sorghum to early combine types. Crop Sci 7:396CrossRefGoogle Scholar
  23. Wang S, Basten CJ, Zeng Z-B (2004) Windows QTL Cartographer 2.0. Department of Statistics, North Carolina State University, Raleigh, NC. (

Copyright information

© Her Majesty the Queen in Rights of Australia 2010

Authors and Affiliations

  • D. R. Jordan
    • 1
  • Emma S. Mace
    • 1
  • R. G. Henzell
    • 1
  • P. E. Klein
    • 2
  • R. R. Klein
    • 3
  1. 1.Queensland Primary Industries and Fisheries, Hermitage Research StationWarwickAustralia
  2. 2.Department of Horticulture and Institute for Plant Genomics and BiotechnologyTexas A&M UniversityCollege StationUSA
  3. 3.USDA-ARS, Southern Plains Agricultural Research CenterCollege StationUSA

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