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Theoretical and Applied Genetics

, Volume 120, Issue 6, pp 1219–1231 | Cite as

An extensive study of the genetic diversity within seven French wine grape variety collections

  • Frédérique Pelsy
  • Stéphanie Hocquigny
  • Ximena Moncada
  • Gérard Barbeau
  • Dominique Forget
  • Patricio Hinrichsen
  • Didier Merdinoglu
Original Paper

Abstract

The process of vegetative propagation used to multiply grapevine varieties produces, in most cases, clones genetically identical to the parental plant. Nevertheless, spontaneous somatic mutations can occur in the regenerative cells that give rise to the clones, leading to consider varieties as populations of clones that conform to a panel of phenotypic traits. Using two sets of nuclear microsatellite markers, the present work aimed at evaluating and comparing the intravarietal genetic diversity within seven wine grape varieties: Cabernet franc, Cabernet Sauvignon, Chenin blanc, Grolleau, Pinot noir, Riesling, Savagnin, comprising a total number of 344 accessions of certified clones and introductions preserved in French repositories. Ten accessions resulted in being either self-progeny, possible offspring of the expected variety or misclassified varieties. Out of the 334 remaining accessions, 83 displayed genotypes different from the varietal reference, i.e., the microsatellite profile shared by the larger number of accessions. They showed a similarity value ranging from 0.923 to 0.992, and thus were considered as polymorphic monozygotic clones. The fraction of polymorphic clones ranged from 2 to 75% depending on the variety and the set of markers, the widest clonal diversity being observed within the Savagnin. Among the 83 polymorphic clones, 29 had unique genotype making them distinguishable; others were classified in 21 groups sharing the same genotype. All microsatellite markers were not equally efficient to show diversity within clone collections and a standard set of five microsatellite markers (VMC3a9, VMC5g7, VVS2, VVMD30, and VVMD 32) relevant to reveal clonal polymorphism is proposed.

Keywords

Microsatellite Marker Allelic Combination Reference Genotype Grapevine Genome Grapevine Variety 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors thank Lucie Bevilacqua-Schehrer, Carine Schmidt, Luz Muñoz, María Herminia Castro, Cindy Ponama, and Gisèle Butterlin for valuable technical assistance. All experiments described in this study comply with the current laws of France. Stéphanie Hocquigny was a doctoral fellow supported by the Conseil Régional d’Alsace and Ximena Moncada by a fellowship from CONICYT/French Embassy at Santiago (Chile). This research was partly supported by grants from ONIVINS and the Ministère de l’Agriculture et de la Pêche (France) and from FONDEF-CONICYT (Chile). We also thank reviewers for suggestions on how to improve the manuscript.

Supplementary material

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Supplementary Table 1 (PDF 42.9 kb)
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Supplementary Table 2 (PDF 78.9 kb)
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Supplementary Table 3a (PDF 66.8 kb)
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Supplementary Table 3b (PDF 68.1 kb)
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Supplementary Table 3c (PDF 63.4 kb)
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Supplementary Table 3d (PDF 60 kb)
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Supplementary Table 3e (PDF 45.9 kb)
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Supplementary Table 3f (PDF 46.5 kb)
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Supplementary Table 3g (PDF 50.6 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Frédérique Pelsy
    • 1
    • 2
  • Stéphanie Hocquigny
    • 1
    • 2
  • Ximena Moncada
    • 3
    • 6
  • Gérard Barbeau
    • 4
  • Dominique Forget
    • 5
  • Patricio Hinrichsen
    • 3
  • Didier Merdinoglu
    • 1
    • 2
  1. 1.INRA, UMR1131ColmarFrance
  2. 2.UMR1131, Université de StrasbourgStrasbourgFrance
  3. 3.Laboratorio de Biotecnología, Centro de Investigación La PlatinaInstituto de Investigaciones Agropecuarias, INIASantiagoChile
  4. 4.INRA, UMT Vinitera, UE1117Beaucouzé CedexFrance
  5. 5.INRA, Domaine de la Grande FerradeVillenave-d’Ornon CedexFrance
  6. 6.Centro de Estudios Avanzados en Zonas Áridas, CEAZAUniversidad de La SerenaLa SerenaChile

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