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Theoretical and Applied Genetics

, Volume 120, Issue 6, pp 1151–1162 | Cite as

Evidence for genetic differentiation and divergent selection in an autotetraploid forage grass (Arrhenatherum elatius)

  • Stefan Georg MichalskiEmail author
  • Walter Durka
  • Anke Jentsch
  • Juergen Kreyling
  • Sven Pompe
  • Oliver Schweiger
  • Evelin Willner
  • Carl Beierkuhnlein
Original Paper

Abstract

The use of local provenances in restoration, agriculture and forestry has been identified as measure to sustain biological diversity and to improve local productivity. However, the delineation of regional provenances is challenging because it requires the identification of well-defined groups based on spatiogenetic differentiation and/or the evidence of local adaptation. In this study, we investigate genetic variation at 186 AFLP loci in 46 European accessions of the important grassland species Arrhenatherum elatius and ask (1) whether genetic variation within accessions differs between European geographical regions; (2) at which spatial scale populations are structured across Europe and (3) whether putatively adaptive markers contribute to this pattern and whether these markers can be related to climatic site conditions. Basic expectations of population genetics are likely to be altered in autotetraploid species, thus, we adopted a band-based approach to estimate genetic diversity and structuring. Compared to other grasses A. elatius showed high genetic diversity and considerable differentiation among accessions (ΦST = 0.24). Accessions separated in a Western European and a Central/Eastern European group, without further structure within groups. A genome scan approach identified four potentially adaptive loci, whose band frequencies correlated significantly with climatic parameters, suggesting that genetic differentiation in A. elatius is also the result of adaptive processes. Knowledge on adaptive loci might in the long run also help to adapt ecosystems to adverse climate change effects through assisted migration of ecotypes rather than introduction of new species.

Keywords

Genetic Differentiation Grassland Species Adaptive Locus Double Reduction Adaptive Genetic Divergence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We would like to express our gratitude to the gene banks of the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) in Gatersleben, the Institute for Agrobotany in Tápiószele, and the Crop Research Institute (RICP) in Prague–Ruzyne for providing seed material of A. elatius.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Stefan Georg Michalski
    • 1
    Email author
  • Walter Durka
    • 1
  • Anke Jentsch
    • 2
  • Juergen Kreyling
    • 3
  • Sven Pompe
    • 4
  • Oliver Schweiger
    • 1
  • Evelin Willner
    • 5
  • Carl Beierkuhnlein
    • 3
  1. 1.Department of Community Ecology (BZF)UFZ, Helmholtz Centre for Environmental ResearchHalleGermany
  2. 2.Geoecology and Physical GeographyUniversity of Koblenz-LandauLandauGermany
  3. 3.BiogeographyUniversity of BayreuthBayreuthGermany
  4. 4.Max Planck Institute for BiogeochemistryJenaGermany
  5. 5.Genebank Satellite Collections NorthLeibniz Institute of Plant Genetics and Crop Plant ResearchMalchow/PoelGermany

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