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Theoretical and Applied Genetics

, Volume 120, Issue 6, pp 1139–1149 | Cite as

Soybean seed lipoxygenase genes: molecular characterization and development of molecular marker assays

  • Julian M. Lenis
  • Jason D. Gillman
  • Jeong Dong Lee
  • J. Grover Shannon
  • Kristin D. BilyeuEmail author
Original Paper

Abstract

Soybean seeds contain three lipoxygenase (Lox) enzymes that are controlled by separate genes, Lox1, Lox2 and Lox3. Lipoxygenases play a role in the development of unpleasant flavors in foods containing soybean by oxidation of polyunsaturated fatty acids. Null alleles for all three enzymes have been identified, lox1, lox2 and lox3, and are known to be inherited as simple recessive alleles. Previous studies determined that a missense mutation rendered Lox2 inactive; however, the genetic cause of either lox1 or lox3 mutation was not known. The objectives of this study were the molecular characterization of both lox1 and lox3 mutant alleles and the development of molecular markers to accelerate breeding for Lox-free soybean varieties. We identified two independent mutant alleles as the genetic causes of the lack of Lox1 in seeds of two lox1 mutant soybean lines. Similarly, a mutant allele that truncates Lox3 in a lox3 mutant soybean line was identified. Molecular markers were designed and confirmed to distinguish mutant, wild type, and heterozygous individuals for Lox1, Lox2 and Lox3 genes. Genotype and Lox phenotype analysis showed a perfect association between the inheritance of homozygous lox mutant alleles and the lack of Lox activity. Molecular characterization of a seed-lipoxygenase-free soybean line led to the discovery that an induced recombination event within the Lox1 gene was responsible for breaking the tight linkage in repulsion phase between mutant alleles at the Lox1 and Lox2 loci. The molecular resources developed in this work should accelerate the inclusion of the lipoxygenase-free trait in soybean varieties.

Keywords

Mutant Allele Soybean Seed Soybean Line Lox3 Gene National Plant Germplasm System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was supported by the USDA-Agricultural research Service and the United Soybean Board.

Supplementary material

122_2009_1241_MOESM1_ESM.pdf (18 kb)
Supplementary material 1 (PDF 17 kb)

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Copyright information

© US Government 2010

Authors and Affiliations

  • Julian M. Lenis
    • 1
  • Jason D. Gillman
    • 2
  • Jeong Dong Lee
    • 3
  • J. Grover Shannon
    • 4
  • Kristin D. Bilyeu
    • 2
    Email author
  1. 1.Division of Plant SciencesUniversity of MissouriColumbiaUSA
  2. 2.Plant Genetics Research Unit, United States Department of Agriculture-Agricultural Research ServiceUniversity of MissouriColumbiaUSA
  3. 3.Division of Plant BiosciencesKyungpook National UniversityDaeguRepublic of Korea
  4. 4.Division of Plant Sciences, Delta CenterUniversity of MissouriPortagevilleUSA

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