Theoretical and Applied Genetics

, Volume 120, Issue 5, pp 1041–1051 | Cite as

Mapping quantitative trait loci for quality factors in an inter-class cross of US and Chinese wheat

Original Paper

Abstract

Wheat quality factors are critical in determining the suitability of wheat (Triticum aestivum L.) for end-use product and economic value, and they are prime targets for marker-assisted selection. Objectives of this study were to identify quantitative trait loci (QTLs) that ultimately influence wheat market class and milling quality. A population of 132 F12 recombinant inbred lines (RILs) was derived by single-seed descent from a cross between the Chinese hard wheat line Ning7840 and the soft wheat cultivar Clark and grown at three Oklahoma locations from 2001 to 2003. Milling factors such as test weight (volumetric grain weight, TW), kernel weight (KW), and kernel diameter (KD) and market class factors such as wheat grain protein content (GPC) and kernel hardness index (HI) were characterized on the basis of a genetic map constructed from 367 SSR and 241 AFLP markers covering all 21 chromosomes. Composite interval mapping identified eight QTLs for TW, seven for KW, six for KD, two each for GPC and HI measured by near-infrared reflectance (NIR) spectroscopy, and four for HI measured by single kernel characterization system. Positive phenotypic correlations were found among milling factors. Consistent co-localized QTLs were identified for TW, KW, and KD on the short arms of chromosomes 5A and 6A. A common QTL was identified for TW and KD on the long arm of chromosome 5A. A consistent major QTL for HI peaked at the Pinb-D1 locus on the short arm of chromosome 5D and explained up to 85% of the phenotypic variation for hardness. We identified QTLs for GPC on 4B and the short arm of 3A chromosomes. The consistency of quality factor QTLs across environments reveals their potential for marker-assisted selection.

Notes

Acknowledgments

We gratefully acknowledge the contributions of Gregory Shaner, Purdue University (RIL population development), and Wayne Whitmore and Connie Shelton for technical assistance in the field experiments and quality trait analysis. This project is partly funded by the NRI of the USDA CSREES, CAP Grant number 2006-55606-16629. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. Contribution no. 09-254-J from the Kansas Agricultural Experiment Station, Manhattan, Kansas, USA.

Supplementary material

122_2009_1232_MOESM1_ESM.doc (86 kb)
Supplementary material (DOC 86 kb)

References

  1. American Assoc. Cereal Chem (1995) Approved methods, 9th edn. AACC, St. PaulGoogle Scholar
  2. Ammiraju JSS, Dholakia BB, Santra DK, Singh H, Lagu MD, Tamhankar SA, Dhaliwal HS, Rao VS, Gupta VS, Ranjekar PK (2001) Identification of inter simple sequence repeat (ISSR) markers associated with seed size in wheat. Theor Appl Genet 102:726–732CrossRefGoogle Scholar
  3. Bai G-H, Kolb FL, Shaner G, Domier LL (1999) Amplified fragment length polymorphism markers linked to a major quantitative trait locus controlling scab resistance in wheat. Phytopathology 89:343–348CrossRefPubMedGoogle Scholar
  4. Börner A, Schumann E, Fürste A, Cöster H, Leithold B, Röder MS, Weber WE (2002) Mapping of quantitative trait loci for agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theor Appl Genet 105:921–936CrossRefPubMedGoogle Scholar
  5. Briggle LW, Reitz LP (1963) Classification of Triticum species and of wheat varieties grown in the United States. ARS-USDA, WashingtonGoogle Scholar
  6. Bushuk W (1998) Wheat breeding for end-product use. Euphytica 100:137–145CrossRefGoogle Scholar
  7. Campbell KG, Bergman CJ, Gualberto DG, Anderson JA, Giroux MJ, Hareland G, Fulcher RG, Sorrells ME, Finney PL (1999) Quantitative trait loci associated with kernel traits in a soft × hard wheat cross. Crop Sci 39:1184–1195Google Scholar
  8. Campbell KG, Finney PL, Bergman CJ, Gualberto DG, Anderson JA, Giroux MJ, Siritunga D, Zhu J-Q, Gendre F, Roué C, Vérel A, Sorrells ME (2001) Quantitative trait loci associated with milling and baking quality in a soft × hard wheat cross. Crop Sci 41:1275–1285CrossRefGoogle Scholar
  9. Carver BF (1994) Genetic implications of kernel NIR hardness on milling and flour quality in bread wheat. J Sci Food Agric 65:125–132CrossRefGoogle Scholar
  10. Cuthbert JL, Somers DJ, Brule-Babel AL, Brown PD, Crow GH (2008) Molecular mapping of quantitative trait loci for yield and yield components in spring wheat (Triticum aestivum L.). Theor Appl Genet 117:595–608CrossRefPubMedGoogle Scholar
  11. Dholakia BB, Ammiraju JSS, Singh H, Lagu MD, Röder MS, Rao VS, Dhaliwal HS, Ranjekar PK, Gupta VS, Weber WE (2003) Molecular marker analysis of kernel size and shape in bread wheat. Plant Breed 122:392–395CrossRefGoogle Scholar
  12. Distelfeld A, Uauy C, Olmos S, Schlatter AR, Dubcovsky J, Fahima T (2004) Microcolinearity between the grain protein content QTL region in wheat chromosome arm 6BS and rice chromosome 2. Funct Integr Genomics 4:59–66CrossRefPubMedGoogle Scholar
  13. Elouafi I, Nachit MM (2004) A genetic linkage map of the Durum × Triticum dicoccoides backcross population based on SSRs and AFLP markers, and QTL analysis for milling traits. Theor Appl Genet 108:401–413CrossRefPubMedGoogle Scholar
  14. Eujayl I, Sorrells ME, Baum M, Wolters P, Powell W (2002) Isolation of EST-derived microsatellite markers for genotyping the A and B genomes of wheat. Theor Appl Genet 104:399–407CrossRefPubMedGoogle Scholar
  15. Galande AA, Tiwari R, Ammiraju JSS, Santra DK, Laga MD, Rao VS, Gupta VS, Misra BK, Nagarajan S, Ranjekar PK (2001) Genetic analysis of kernel hardness in bread wheat using PCR-based markers. Theor Appl Genet 103:601–606CrossRefGoogle Scholar
  16. Gibson LR, McCluskey PJ, Tilley KA, Paulsen GM (1998) Quality of hard red winter wheat grown under high temperature conditions during maturation and ripening. Cereal Chem 75:421–427CrossRefGoogle Scholar
  17. Giroux MJ, Morris CF (1998) Wheat grain hardness results from highly conserved mutations in the friabilin components puroindoline a and b. Proc Natl Acad Sci 95:6262–6266CrossRefPubMedGoogle Scholar
  18. Groos C, Robert N, Bervas E, Charmet G (2003) Genetic analysis of grain protein-content, grain yield, and thousand-kernel weight in bread wheat. Theor Appl Genet 106:1032–1040PubMedGoogle Scholar
  19. Guyomarc’h H, Sourdille P, Edwards KJ, Bernard M (2002) Studies of the transferability of microsatellite derived from Triticum taushchii to hexaploid wheat and to diploid related species using amplification, hybridization and sequence comparisons. Theor Appl Genet 105:736–744CrossRefPubMedGoogle Scholar
  20. Huang X-Q, Cloutier S, Lycar L, Radovanovic N, Humphreys DG, Noll JS, Somers DJ, Brown PD (2006) Molecular detection of QTLs for agronomic and quality traits in a double haploid population derived from two Canidian wheats (Triticum aestivium L.). Theor Appl Genet 113:753–766CrossRefPubMedGoogle Scholar
  21. Khan IA, Procunier JD, Humphreys DG, Tranquilli G, Schlatter AR, Marcucci-Poltri S, Frohberg R, Dubcovsky J (2000) Development of PCR-based markers for a high grain protein content gene from Triticum turgidum ssp. dicoccoides transferred to bread wheat. Crop Sci 40:518–524CrossRefGoogle Scholar
  22. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175Google Scholar
  23. Lillemo M, Ringlund K (2002) Impact of puroindoline b alleles on the genetic variation for hardness in soft × hard wheat crosses. Plant Breed 121:210–217CrossRefGoogle Scholar
  24. Liu S, Cai S, Graybosch R, Chen C, Bai G (2008) Quantitative trait loci for resistance to pre-harvest sprouting in U.S. hard white winter wheat Rio Blanco. Theor Appl Genet 117:691–699CrossRefPubMedGoogle Scholar
  25. Marshall DR, Ellison FW, Mares DJ (1984) Effects of grain shape and size on milling yields in wheat. I Theoretical analysis based on simple geometric models. Aust J Agric Res 35:619–630CrossRefGoogle Scholar
  26. Martin JM, Frohberg RC, Morris CF, Talbert LE, Giroux MJ (2001) Milling and bread baking traits associated with puroindoline sequence type in hard red spring wheat. Crop Sci 41:228–234Google Scholar
  27. Marza F, Bai G-H, Carver BF, Zhou W-C (2006) Integrated QTL mapping for yield and related traits in wheat population: Ning7840 × Clark. Theor Appl Genet 112:688–698CrossRefPubMedGoogle Scholar
  28. Morris GF (2002) Puroindolines: the molecular genetic basis of wheat grain hardness. Plant Mol Biol 48:633–647CrossRefPubMedGoogle Scholar
  29. Morris CF, Rose SP (1996) Wheat. In: Henry RJ, Kettlewell PS (eds) Cereal grain quality. Chapman & Hall, LondonGoogle Scholar
  30. Ohm HW, Shaner G, Foster JE, Patterson FL, Buechley G (1988) Registration of ‘Clark’ wheat. Crop Sci 28:1031–1032CrossRefGoogle Scholar
  31. Olmos S, Distelfeld A, Chicaiza O, Schlatter AR, Fahima T, Echenique V, Dubcovsky J (2003) Precise mapping of a locus affecting grain protein content in durum wheat. Theor Appl Genet 107:1243–1251CrossRefPubMedGoogle Scholar
  32. Pestsova EG, Ganal MW, Röder MS (2000) Isolation and mapping of microsatellite markers specific for the D-genome of bread wheat. Genome 43:689–697CrossRefPubMedGoogle Scholar
  33. Prasad, M, Varshney RK, Kumar A, Balyan HS, Sharma PC, Edwards KJ, Singh H, Dhaliwal HS, Roy JK, Gupta PK (1999) A microsatellite marker associated with a QTL for grain protein content on chromosome arm 2DL of bread wheat. Theor Appl Genet 99:341–345Google Scholar
  34. Röder MS, Korsun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  35. Schuler SF, Bacon RK, Gbur EE (1994) Kernel and spike character influence on test weight of soft red winter wheat. Crop Sci 34:1309–1313CrossRefGoogle Scholar
  36. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114CrossRefPubMedGoogle Scholar
  37. Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J, Gill BS, Ward R, Cregan PB (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550–560CrossRefPubMedGoogle Scholar
  38. Sourdille P, Perretant MR, Charment G, Leroy P, Gautier MF, Joudrier P, Nelson JC, Sorrells ME, Bernard M (1996) Linkage between RFLP markers and genes affecting kernel hardness in wheat. Theor Appl Genet 93:580–586CrossRefGoogle Scholar
  39. Sourdille P, Cadalen T, Guyomarc’h H, Snape JW, Perretant MR, Charmet G, Boeuf C, Bernard S, Bernard M (2003) An update of the Courtot/Chinese Spring intervarietal molecular marker linkage map for the QTL detection of agronomic traits in wheat. Theor Appl Genet 106:530–538PubMedGoogle Scholar
  40. Uauy C, Distelfeld A, Fahima T, Blechl A, Dubcovsky J (2006) A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science 314:1298–1301CrossRefPubMedGoogle Scholar
  41. Van Ooijen JW, Voorrips RE (2001) Joinmap 3.0 software for the calculation of genetic linkage maps. Plant Research International, WageningenGoogle Scholar
  42. Varshney RK, Prasad M, Roy JK, Kumar N, Singh H, Dhaliwal HS, Balyan HS, Gupta PK (2000) Identification of eight chromosomes and a microsatellite marker on 1AS associated with QTL for grain weight in bread wheat. Theor Appl Genet 100:1290–1294CrossRefGoogle Scholar
  43. Wang S, Basten CJ, Zeng ZB (2004) Windows QTL cartographer. Version 2.0. Department of Statistics, North Carolina State Univ, RaleighGoogle Scholar
  44. Wiersma JJ, Busch RH, Fulcher GG, Hareland GA (2001) Recurrent selection for kernel weight in spring wheat. Crop Sci 41:999–1005CrossRefGoogle Scholar
  45. Wrigley CW, Asenstorfer R, Batey I, Cornish G, Day L, Mares D, Mrva K (2009) The biochemical and molecular basis of wheat quality. In: Carver BF (ed) Wheat: science and trade. Wiley, AmesGoogle Scholar
  46. Yamazaki WT, Briggle LW (1969) Components of test weight in soft wheat. Crop Sci 9:457–459CrossRefGoogle Scholar
  47. Yan W, Kang MS (2003) GGE biplot analysis, a graphical tool for breeders, geneticists, and agronomists. CRC Press, Boca RatonGoogle Scholar
  48. Zanetti S, Winzeler M, Feuillet C, Keller B, Messmer M (2001) Genetic analysis of bread-making quality in wheat and spelt. Plant Breed 120:13–19CrossRefGoogle Scholar

Copyright information

© US Government 2009

Authors and Affiliations

  1. 1.Department of AgronomyKansas State UniversityManhattanUSA
  2. 2.USDA-ARS, Plant Science and Entomology Research UnitManhattanUSA
  3. 3.Department of Plant and Soil SciencesOklahoma State UniversityStillwaterUSA
  4. 4.Plant Biotechnology InstituteJiangsu Academy of Agricultural ScienceNanjingChina

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