Dissection of a QTL reveals an adaptive, interacting gene complex associated with transgressive variation for flowering time in rice
- 401 Downloads
A days to heading QTL (dth1.1) located on the short arm of rice chromosome 1 was sub-divided into eight sub-introgression lines (SILs) to analyze the genetic basis of transgressive variation for flowering time. Each SIL contained one or more introgression(s) from O. rufipogon in the genetic background of the elite Oryza sativa cultivar, Jefferson. Each introgression was defined at high resolution using molecular markers and those in the dth1.1 region were associated with the presence of one or more flowering time genes (GI, SOC1, FT-L8, EMF1, and PNZIP). SILs and controls were evaluated for flowering time under both short- and long-day growing conditions. Under short-day lengths, lines with introgressions carrying combinations of linked flowering time genes (GI/SOC1, SOC1/FT-L8, GI/SOC1/FT-L8 and EMF1/PNZIP) from the late parent, O. rufipogon, flowered earlier than the recurrent parent, Jefferson, while recombinant lines carrying smaller introgressions marked by the presence of GI, SOC1, EMF1 or PNZIP alone no longer flowered early. Under long-day length, lines carrying SOC1/FT-L8, SOC1 or PNZIP flowered early, while those carrying GI or EMF1 delayed flowering. Across all experiments and in the field, only SIL_SOC1/FT-L8 was consistently early. A preliminary yield evaluation indicated that the transgressive early flowering observed in several of the SILs was also associated with a measurable and positive effect on yield. These SILs represent a new source of variation that can be used in breeding programs to manipulate flowering time in rice cultivars without the reduction in yield that is often associated with early maturing phenotypes.
KeywordsFlowering Time Early Flowering Photoperiod Sensitivity Ratoon Crop Tropical Japonica
We would like to thank Mrs. Marie Lavallard and the Department of Plant Breeding and Genetics for financial support for the PhD program of LFM (via a Frank T. Rhodes Fellowship), the National Science Foundation (Grant DBI #0606461 to SMc) for research funding, Lisa Polewczak for assistance with the field work in the Dale Bumpers National Rice Research Center in Stuttgart, AR and the USDA-ARS Rice Research Unit in Beaumont, TX. We express our gratitude to Michael Thomson and Jeremy Edwards for seeds from the parental pre-SILs provided for this study, and to Michael Gore and Walter de Jong for constructive comments and suggestions during manuscript preparation. We gratefully acknowledge Lois Swales for her assistance in preparing the figures and for formatting the manuscript.
- Altschul SF, Gish W, Miller W, Myer EW, Lipman DJ (1990) Basic local alignment search tool. Mol Biol 215:403–410Google Scholar
- Doi K, Yoshimura A, Iwata N (1998) RFLP mapping and QTL analysis of heading date and pollen sterility using backcross populations between Oryza sativa L. and Oryza glaberrima Steud. Breed Sci 48:395–399Google Scholar
- Garner WW, Allard HA (1920) Effect of the relative length of day and night and other factors of the environment on growth and reproduction in plants. J Agric Res 18:553–606Google Scholar
- Kohn JR, Leyva N, Dossey R, Sobral B, Morishima H (1997) Quantitative trait locus analysis of trait variation among annual and perennial ecotypes of Oryza rufipogon. Int Rice Res Notes 22:4–5Google Scholar
- Thomson MJ, Tai TH, McClung AM, Lai XH, Hinga ME, Lobos KB, Xu Y, Martinez CP, McCouch SR (2003) Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson. Theor Appl Genet 107:479–493CrossRefPubMedGoogle Scholar
- Yano M, Katayose Y, Ashikari M, Yamanouchi U, Monna L, Fuse T, Baba T, Yamamoto K, Umehara Y, Nagamura Y, Sasaki T (2000) HD1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell 12:2473–2484CrossRefPubMedGoogle Scholar