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Theoretical and Applied Genetics

, Volume 120, Issue 3, pp 679–689 | Cite as

Development of diagnostic markers for use in breeding potatoes resistant to Globodera pallida pathotype Pa2/3 using germplasm derived from Solanum tuberosum ssp. andigena CPC 2802

  • Claire Moloney
  • Denis Griffin
  • Peter W. Jones
  • Glenn J. Bryan
  • Karen McLean
  • John E. Bradshaw
  • Dan MilbourneEmail author
Original Paper

Abstract

Quantitative resistance to Globodera pallida pathotype Pa2/3, originally derived from Solanum tuberosum ssp. andigena Commonwealth Potato Collection (CPC) accession 2802, is present in several potato cultivars and advanced breeding lines. One genetic component of this resistance, a large effect quantitative trait locus (QTL) on linkage group IV (which we have renamed GpaIV adg s ) has previously been mapped in the tetraploid breeding line 12601ab1. In this study, we show that GpaIV adg s is also present in a breeding line called C1992/31 via genetic mapping in an F1 population produced by crossing C1992/31 with the G. pallida susceptible cultivar Record. C1992/31 is relatively divergent from 12601ab1, confirming that GpaIV adg s is an ideal target for marker-assisted selection in currently available germplasm. To generate markers exhibiting diagnostic potential for GpaIV adg s , three bacterial artificial chromosome clones were isolated from the QTL region, sequenced, and used to develop 15 primer sets generating single-copy amplicons, which were examined for polymorphisms exhibiting linkage to GpaIV adg s in C1992/31. Eight such polymorphisms were found. Subsequently, one insertion/deletion polymorphism, three single nucleotide polymorphisms and a specific allele of the microsatellite marker STM3016 were shown to exhibit diagnostic potential for the QTL in a panel of 37 potato genotypes, 12 with and 25 without accession CPC2082 in their pedigrees. STM3016 and one of the SNP polymorphisms, C237(119), were assayed in 178 potato genotypes, arising from crosses between C1992/31 and 16 G. pallida susceptible genotypes, undergoing selection in a commercial breeding programme. The results suggest that the diagnostic markers would most effectively be employed in MAS-based approaches to pyramid different resistance loci to develop cultivars exhibiting strong, durable resistance to G. pallida pathotype Pa2/3.

Keywords

Quantitative Trait Locus Effect Quantitative Trait Locus Resistance Quantitative Trait Locus Scottish Crop Research Institute T3683 Population 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

CM was supported by a Teagasc Walsh Fellowship during the course of this study. DM is supported by the National Development Plan (NPD) of Ireland. The potato breeding programme at Oak Park (DG) is supported by the NDP and Irish Potato Marketing Limited. GJB and JEB acknowledge financial support of the Scottish Government Rural Environment Research and Analysis Directorate (RERAD). The authors wish to thank Dr Della Milbourne for help with the figures and proofreading the manuscript, Dr Helena Meally for help with fluorescent genotyping of STM3016 and Emmet Dalton for genotyping the HC and SPUD1636 markers.

Supplementary material

122_2009_1185_MOESM1_ESM.doc (29 kb)
(DOC 29 kb)

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Claire Moloney
    • 1
  • Denis Griffin
    • 1
  • Peter W. Jones
    • 2
  • Glenn J. Bryan
    • 3
  • Karen McLean
    • 3
  • John E. Bradshaw
    • 3
  • Dan Milbourne
    • 1
    Email author
  1. 1.Crops Research Centre, TeagascCarlowIreland
  2. 2.Department of Zoology, Ecology and Plant ScienceUniversity College CorkNorth MallIreland
  3. 3.Genetics ProgrammeScottish Crop Research InstituteDundeeUK

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