Theoretical and Applied Genetics

, Volume 120, Issue 1, pp 31–43 | Cite as

Development of public immortal mapping populations, molecular markers and linkage maps for rapid cycling Brassica rapa and B. oleracea

  • Federico Luis Iniguez-LuyEmail author
  • Lewis Lukens
  • Mark W. Farnham
  • Richard M. Amasino
  • Thomas C. Osborn
Original Paper


Publicly available genomic tools help researchers integrate information and make new discoveries. In this paper, we describe the development of immortal mapping populations of rapid cycling, self-compatible lines, molecular markers, and linkage maps for Brassica rapa and B. oleracea and make the data and germplasm available to the Brassica research community. The B. rapa population consists of 160 recombinant inbred (RI) lines derived from the cross of highly inbred lines of rapid cycling and yellow sarson B. rapa. The B. oleracea population consists of 155 double haploid (DH) lines derived from an F1 cross between two DH lines, rapid cycling and broccoli. A total of 120 RFLP probes, 146 SSR markers, and one phenotypic trait (flower color) were used to construct genetic linkage maps for both species. The B. rapa map consists of 224 molecular markers distributed along 10 linkage groups (A1–A10) with a total distance of 1125.3 cM and a marker density of 5.7 cM/marker. The B. oleracea genetic map consists of 279 molecular markers and one phenotypic marker distributed along nine linkage groups (C1–C9) with a total distance of 891.4 cM and a marker density of 3.2 cM/marker. A syntenic analysis with Arabidopsis thaliana identified collinear genomic blocks that are in agreement with previous studies, reinforcing the idea of conserved chromosomal regions across the Brassicaceae.


Mapping Population Double Haploid Segregation Distortion Rapid Cycling Brassica Crop 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We would like to thank Dr. Maria Laura Federico for her critical reading of the manuscript and Dr. Carlos Quiros and two anonymous referees for their helpful and constructive comments which have improved the contents of this manuscript. The authors would also like to thank Robert Vogelzang and Amy Van der Voort for their technical support.

Supplementary material

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Federico Luis Iniguez-Luy
    • 1
    • 2
    Email author
  • Lewis Lukens
    • 3
  • Mark W. Farnham
    • 4
  • Richard M. Amasino
    • 5
  • Thomas C. Osborn
    • 6
  1. 1.Agri aquaculture Nutritional Genomic Center (CGNA), Plant Biotechnology Unit (UBP)INIA-CarillancaTemucoChile
  2. 2.Department of AgronomyUniversity of Wisconsin-MadisonMadisonUSA
  3. 3.Department of Plant AgricultureUniversity of GuelphGuelphCanada
  4. 4.USDA-ARSCharlestonUSA
  5. 5.Department of BiochemistryUniversity of Wisconsin-MadisonMadisonUSA
  6. 6.Seminis Vegetables SeedsWoodlandUSA

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