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Theoretical and Applied Genetics

, Volume 119, Issue 7, pp 1281–1288 | Cite as

Molecular mapping of leaf rust resistance gene Rph14 in Hordeum vulgare

  • Prashant G. Golegaonkar
  • Haydar Karaoglu
  • Robert F. ParkEmail author
Original Paper

Abstract

An incompletely dominant gene conferring resistance to Puccinia hordei, Rph14, identified previously in an accession of Hordeum vulgare, confers resistance to all known pathotypes of P. hordei in Australia. Knowledge of the chromosomal location of Rph14 and the identification of DNA markers closely linked to it will facilitate combining it with other important leaf rust resistance genes to achieve long lasting resistance. The inheritance of Rph14 was confirmed using 146 and 106 F3 lines derived from the crosses ‘Baudin’/‘PI 584760’ (Rph14) and ‘Ricardo’/‘PI 584760’ (Rph14), respectively. Bulk segregant analysis on DNA from the parental genotypes and resistant and susceptible DNA bulks using DArT markers located Rph14 to the short arm of chromosome 2H. DArT marker bPb-1664 was identified as having the closest genetic association with Rph14. PCR based marker analysis identified a single SSR marker, Bmag692, linked closely to Rph14 at a map distance of 2.1 and 3.8 cm in the ‘Baudin’/‘PI 584760’and ‘Ricardo’/‘PI 584760’ populations, respectively.

Keywords

Simple Sequence Repeat Marker Doubled Haploid DArT Marker Bulk Segregant Analysis Amplify Fragment Length Polymorphism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The senior author would like to sincerely thank the Grains Research and Development Corporation for the provision of a Postgraduate Research Scholarship that enabled these studies to be conducted. The authors would like to thank Mr M. Williams and Mr P. Kavanagh for valuable technical assistance in the course of this study, and acknowledge the support provided by the University of Sydney.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Prashant G. Golegaonkar
    • 1
    • 2
  • Haydar Karaoglu
    • 1
  • Robert F. Park
    • 1
    Email author
  1. 1.Plant Breeding Institute CobbittyUniversity of SydneyCamdenAustralia
  2. 2.Monsanto India LtdBangaloreIndia

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