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Theoretical and Applied Genetics

, Volume 119, Issue 7, pp 1237–1246 | Cite as

High-resolution mapping of two rice brown planthopper resistance genes, Bph20(t) and Bph21(t), originating from Oryza minuta

  • Md Lutfor Rahman
  • Wenzhu Jiang
  • Sang Ho Chu
  • Yongli Qiao
  • Tae-Ho Ham
  • Mi-Ok Woo
  • Joohyun Lee
  • M. Sakina Khanam
  • Joong-Hyoun Chin
  • Ji-Ung Jeung
  • D. S. Brar
  • K. K. Jena
  • Hee-Jong KohEmail author
Original Paper

Abstract

Brown planthopper (BPH) is one of the most destructive insect pests of rice. Wild species of rice are a valuable source of resistance genes for developing resistant cultivars. A molecular marker-based genetic analysis of BPH resistance was conducted using an F2 population derived from a cross between an introgression line, ‘IR71033-121-15’, from Oryza minuta (Accession number 101141) and a susceptible Korean japonica variety, ‘Junambyeo’. Resistance to BPH (biotype 1) was evaluated using 190 F3 families. Two major quantitative trait loci (QTLs) and two significant digenic epistatic interactions between marker intervals were identified for BPH resistance. One QTL was mapped to 193.4-kb region located on the short arm of chromosome 4, and the other QTL was mapped to a 194.0-kb region on the long arm of chromosome 12. The two QTLs additively increased the resistance to BPH. Markers co-segregating with the two resistance QTLs were developed at each locus. Comparing the physical map positions of the two QTLs with previously reported BPH resistance genes, we conclude that these major QTLs are new BPH resistance loci and have designated them as Bph20(t) on chromosome 4 and Bph21(t) on chromosome 12. This is the first report of BPH resistance genes from the wild species O. minuta. These two new genes and markers reported here will be useful to rice breeding programs interested in new sources of BPH resistance.

Keywords

Wild Species International Rice Research Institute Major QTLs Brown Planthopper Resistance QTLs 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was supported by a grant (code# CG3111) from the Crop Functional Genomics Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Republic of Korea. We are also thankful to Rural Development Administration, Korea for providing necessary greenhouse facilities for BPH bioassay.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Md Lutfor Rahman
    • 1
  • Wenzhu Jiang
    • 1
  • Sang Ho Chu
    • 1
  • Yongli Qiao
    • 1
  • Tae-Ho Ham
    • 1
  • Mi-Ok Woo
    • 1
  • Joohyun Lee
    • 1
  • M. Sakina Khanam
    • 1
    • 2
  • Joong-Hyoun Chin
    • 3
  • Ji-Ung Jeung
    • 5
  • D. S. Brar
    • 3
  • K. K. Jena
    • 4
  • Hee-Jong Koh
    • 1
    Email author
  1. 1.Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute of Agriculture and Life SciencesSeoul National UniversitySeoulKorea
  2. 2.Department of Crop PhysiologyBangladesh Institute of Nuclear AgricultureMymensinghBangladesh
  3. 3.Plant Breeding, Genetics and Biotechnology DivisionInternational Rice Research InstituteManilaPhilippines
  4. 4.Plant Breeding, Genetics and Biotechnology DivisionInternational Rice Research Institute, C/o IRRI-Korea Office, National Institute of Crop ScienceSuwonKorea
  5. 5.Rice Research DivisionNational Institute of Crop ScienceSuwonKorea

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