Theoretical and Applied Genetics

, Volume 119, Issue 7, pp 1237–1246 | Cite as

High-resolution mapping of two rice brown planthopper resistance genes, Bph20(t) and Bph21(t), originating from Oryza minuta

  • Md Lutfor Rahman
  • Wenzhu Jiang
  • Sang Ho Chu
  • Yongli Qiao
  • Tae-Ho Ham
  • Mi-Ok Woo
  • Joohyun Lee
  • M. Sakina Khanam
  • Joong-Hyoun Chin
  • Ji-Ung Jeung
  • D. S. Brar
  • K. K. Jena
  • Hee-Jong KohEmail author
Original Paper


Brown planthopper (BPH) is one of the most destructive insect pests of rice. Wild species of rice are a valuable source of resistance genes for developing resistant cultivars. A molecular marker-based genetic analysis of BPH resistance was conducted using an F2 population derived from a cross between an introgression line, ‘IR71033-121-15’, from Oryza minuta (Accession number 101141) and a susceptible Korean japonica variety, ‘Junambyeo’. Resistance to BPH (biotype 1) was evaluated using 190 F3 families. Two major quantitative trait loci (QTLs) and two significant digenic epistatic interactions between marker intervals were identified for BPH resistance. One QTL was mapped to 193.4-kb region located on the short arm of chromosome 4, and the other QTL was mapped to a 194.0-kb region on the long arm of chromosome 12. The two QTLs additively increased the resistance to BPH. Markers co-segregating with the two resistance QTLs were developed at each locus. Comparing the physical map positions of the two QTLs with previously reported BPH resistance genes, we conclude that these major QTLs are new BPH resistance loci and have designated them as Bph20(t) on chromosome 4 and Bph21(t) on chromosome 12. This is the first report of BPH resistance genes from the wild species O. minuta. These two new genes and markers reported here will be useful to rice breeding programs interested in new sources of BPH resistance.


Wild Species International Rice Research Institute Major QTLs Brown Planthopper Resistance QTLs 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This research was supported by a grant (code# CG3111) from the Crop Functional Genomics Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Republic of Korea. We are also thankful to Rural Development Administration, Korea for providing necessary greenhouse facilities for BPH bioassay.


  1. Alam SN, Cohen MB (1998) Detection and analysis of QTLs for resistance to the brown planthopper, Nilaparvata lugens, in a double-haploid rice population. Theor Appl Genet 97:1370–1379CrossRefGoogle Scholar
  2. Amante AD, Sitch LA, Nelson R, Dalmacio RD, Oliva NP, Aswidi H, Leung H (1998) Transfer of bacterial blight and blast resistance from the tetraploid wild rice Oryza minuta to cultivated rice O. sativa. Theor Appl Genet 84:345–354Google Scholar
  3. Brar DS, Khush GS (1997) Alien introgression in rice. Plant Mol Biol 35:35–47CrossRefPubMedGoogle Scholar
  4. Chelliah S, Bharathi M (1993) Biotypes of the brown planthopper, Nilaparvata lugens (Homoptera: Delphacidae)—host influenced biology and behavior. In: Ananthakrishnan TN, Raman A (eds) Chemical ecology of phytopathogous insects. International Science Publishers, New York, pp 133–148Google Scholar
  5. Chen JW, Wang L, Pang XF, Pan QH (2006) Genetic analysis and fine mapping of a rice brown planthopper (Nilaparbata lugens stal) resistance gene bph19(t). Mol Gen Genomics 275:321–329CrossRefGoogle Scholar
  6. Chin JH, Kim JH, Jiang W, Chu SH, Woo MO, Han L, Brar D, Koh HJ (2007) Identification of subspecies-specific STS markers and their association with segregation distortion in rice (Oryza sativa L.). J Crop Sci Biotech 10(3):175–184Google Scholar
  7. Feuer R (1976) Biotype 2 brown planthopper in the Philippines. Int Rice Res New 1(1):15Google Scholar
  8. Heinrichs EA (1979) Control of leafhopper and planthopper vectors of rice viruses. In: Moramorosch K, Arris KF (eds) Leafhopper vectors and planthopper disease agents. Academic Press, New York, pp 529–558Google Scholar
  9. Heinrichs EA, Aquino GB, Chelliah S (1982) Resurgence of Nilaparvata lugens (Stål) populations as influenced by method and timing of insecticide applications in lowland rice. Environ Entomol 11:78–84Google Scholar
  10. Hirabayashi H, Ogawa T (1995) RFLP Mapping of Bph-1 (Brown planthopper resistance gene) in rice. Breed Sci 45:369–371Google Scholar
  11. Hirabayashi H, Kaji R, Angeles ER, Ogawa T, Brar DS, Khush GS (1999) RFLP analysis of a new gene for resistance to brown planthopper derived from O. officinalis on rice chromosome 4. (abstract in Japanese). Breed Sci 48(Suppl 1):48Google Scholar
  12. Huang Z, He G, Shu L, Li X, Zhang Q (2001) Identification and mapping of two brown planthopper resistance genes in rice. Theor Appl Genet 102:929–934CrossRefGoogle Scholar
  13. Huynh NV, Nhung HT (1988) High virulence of new brown planthopper (BPH) populations in the Mekong Delta, Vietnam. Int Rice Res New 13(5):16Google Scholar
  14. Ikeda R (1985) Studies of the inheritance of resistance to the rice brown planthopper (Nilaparvata lugens Stål) and the breeding of resistance rice cultivar. Bull Natl Agric Res Cent 3:1–54 (In Japanese)Google Scholar
  15. Ikeda R, Vaughan DA (1991) The distribution of resistance genes to the brown planthopper in rice germplasm. RGN 8:1–3Google Scholar
  16. IRRI (1988) Standard evaluation system for rice. IRRI, ManilaGoogle Scholar
  17. Ishii T, Brar DS, Multani DS, Khush GS (1994) Molecular tagging of genes for brown planthopper resistance and earliness introgressed from Oryza australiensis into cultivated rice, O. sativa. Genome 37:217–221CrossRefPubMedGoogle Scholar
  18. Jairin J, Phengrat K, Teangdeerith S, Vanavichit A, Toojinda T (2007) Mapping of a broad-spectrum brown planthopper resistance gene, Bph3, on rice chromosome 6. Mol Breed 19:35–44CrossRefGoogle Scholar
  19. Jena KK, Pasalu IC, Rao YK, Varalaxmi Y, Krishnaiah K, Khush GS, Kochert G (2002) Molecular tagging of a gene for resistance to brown planthopper in rice (Oryza sativa L.). Euphytica 129:81–88CrossRefGoogle Scholar
  20. Jena KK, Jeung JU, Lee JH, Choi HC, Brar DS (2006) High-resolution mapping of a new brown planthopper (BPH) resistance gene, Bph18(t), and marker-assisted selection for BPH resistance in rice (Oryza sativa L.). Theor Appl Genet 112:288–297CrossRefPubMedGoogle Scholar
  21. Jeon YH, Ahn SN, Choi HC, Hahn TR, Moon HP (1999) Identification of a RAPD marker linked to a brown planthopper resistance gene. Euphytica 107:23–28CrossRefGoogle Scholar
  22. Kawaguchi M, Murata K, Ishii T, Takumi S, Mori N, Nakamura C (2001) Assignment of a brown planthopper (Nilaparvata lugens Stal) resistance gene bph4 to rice chromosome 6. Breed Sci 51:13–18CrossRefGoogle Scholar
  23. Khush GS (1979) Genetics and breeding for resistance to brown planthopper. In:   IRRI (ed) Brown planthopper: threat to rice production in Asia. IRRI, Los Banõs, Philippines, pp 321–322Google Scholar
  24. Khush GS (1984) Breeding rice for resistance to insects. Prot Ecol 7:147–165Google Scholar
  25. Khush GS, Karim AR, Angeles EG (1985) Genetics of resistance of rice cultivar ARC10550 to Bangladesh brown planthopper biotype. J Genet 64(2):121–125CrossRefGoogle Scholar
  26. Kim SM, Sohn JK (2005) Identification of a rice gene (Bph1) conferring resistance to brown planthopper (Nilaparvata lugens Stål) using STS markers. Mol Cells 20(1):30–34PubMedGoogle Scholar
  27. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175Google Scholar
  28. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181CrossRefPubMedGoogle Scholar
  29. Liu GQ, Yan H, Fu Q, Qian Q, Zhang ZT, Zhai WX, Zhu LH (2001) Mappping of a new gene for brown planthopper resistance in cultivated rice introgressed from Oryza eichingeri. Chin Sci Bull 46:738–742Google Scholar
  30. Medrano FG, Heinrichs EA (1985) Response of resistant rices to brown planthoppers (BPH) collected in Mindanao, Philippines. Int Rice Res New 10(6):14–15Google Scholar
  31. Mochida O, Oka IN, Dandi S, Harahap Z, Sutjipto P, Beachell HM (1977) IR26 found susceptible to the brown planthopper in North Sumatra, Indonesia. Int Rice Res New 2(5):10–11Google Scholar
  32. Murata K, Fujiwara M, Kaneda C, Takumi S, Mori N, Nakamura C (1998) RFLP mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene bph2 of indica rice introgressed into a japonica breeding line ‘Norin-PL4. Genes Genet Syst 73:359–364CrossRefGoogle Scholar
  33. Murata K, Fujiwara M, Murai H, Takumi S, Mori N, Nakamura C (2001) Mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph9 on the long arm of rice chromosome 12. Cereal Res Commun 29:245–250Google Scholar
  34. Murray MG, Thompson WF (1980) Rapid isolation of high molecular-weight plant DNA. Nucleic Acids Res 8:4321–4325CrossRefPubMedGoogle Scholar
  35. Panaud O, Chen X, McCouch SR (1996) Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Gen Genet 252:597–607PubMedGoogle Scholar
  36. Panda N and Khush GS (1995) Host plant resistance to insects. CAB International, Wallingford, 431 ppGoogle Scholar
  37. Park YD, Song YH (1988) Studies on the distribution of the brown planthopper (Nilaparvata lugens Stal) biotypes migrated in the southern regions of Korea. Korean J Appl Entomol 27(2):63–67Google Scholar
  38. Park DS, Lee SK, Lee JH, Song MY, Song SY, Kwak DY, Yeo US, Jeon NS, Park SK, Yi G, Song YC, Nam MH, Ku YC, Jeon JS (2007) The identification of candidate rice genes that confer resistance to the brown planthopper (Nilaparvata lugens) through representational difference analysis. Theor Appl Genet 115:357–547CrossRefGoogle Scholar
  39. Pathak MD, Khush GS (1979) Studies of varietal resistance in rice to the brown planthopper at the International Rice Research Institute. In: IRRI (ed) Brown planthopper: threat to rice production in Asia. pp 285–301.IRRI, Los Banõs, PhilippinesGoogle Scholar
  40. Pathak MD, Cheng CH, Fortuno ME (1969) Resistance to Nephotettix impictiveps and Nilaparvata lugens in varieties of rice. Nature 223:502–504CrossRefGoogle Scholar
  41. Qiao Y, Jiang W, Rahman ML, Chu SH, Piao R, Han L, Koh HJ (2008) Comparison of molecular linkage maps and QTLs for morphological traits in two reciprocal backcross populations of rice. Mol Cells 25(3):417–427PubMedGoogle Scholar
  42. Rahman ML, Chu SH, Choi MS, Qiao YL, Jiang W, Piao R, Khanam S, Cho YI, Jeung JU, Jena KK, Koh HJ (2007) Identification of QTLs for some agronomic traits in rice using an introgression line from Oryaza minuta. Mol Cells 24(1):16–26PubMedGoogle Scholar
  43. Ren X, Wang X, Yuan H, Weng Q, Zhu L, He G (2004) Mapping quantitative trait loci and expressed sequence tags related to brown planthopper resistance in rice. Plant Breed 123:342–348CrossRefGoogle Scholar
  44. Renganayaki K, Feitz AK, Sadasivam S, Pammi S, Harrington SE, McCouch SR, Kumar SM, Reddy AS (2002) Mapping and progress toward map-based cloning of brown planthopper biotype-4 resistance gene introgressed from Oryza officinalis into cultivated rice, O. sativa. Crop Sci 42:2112–2117CrossRefGoogle Scholar
  45. Rivera CT, Ou SH, Lida TT (1966) Grassy stunt disease of rice and its transmission by Nilaparvata lugens (Stal). Plant Dis Rep 50:453–456Google Scholar
  46. Sharma PN, Ketipearachchi Y, Murata K, Torii A, Takumi S, Mori N, Nakamura C (2003) RFLP/AFLP mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph1 in rice. Euphytica 129(1):109–117CrossRefGoogle Scholar
  47. Sharma PN, Torii A, Takumi S, Mori N, Nakamura C (2004) Marker-assisted pyramiding of brown planthopper (Nilaparvata lugens Stål) resistance genes Bph1 and Bph2 on rice chromosome 12. Heriditas 140:61–69CrossRefGoogle Scholar
  48. Sogawa K, Soekirno Raksadinata Y (1987) New genetic makeup of brown planthopper (BPH) populations in Central Java, Indonesia. Int Rice Res New 12(6):29–30Google Scholar
  49. Soundararajan RP, Kadirvel P, Gunathilagaraj K, Maheswaran M (2004) Mapping of Quantitative trait loci associated with resistance to brown planthopper in rice by means of a double haploid population. Crop Sci 44:2214–2220Google Scholar
  50. Stapley JH, May-Jackson YY, Golden WG (1979) Varietal resistance to the brown planthopper in the Solomon Islands. In: International Rice Research Institute (ed) Brown planthopper: threat to rice production in Asia. International Rice Research Institute, Los Banõs, pp 233–239Google Scholar
  51. Su CC, Cheng XN, Zhai HQ, Wan JM (2002) Detection and analysis of QTL for resistance to brown planthopper, Nilaparvata lugens (Stål), in rice (Oryza sativa L.), using backcross inbred lines. Acta Genetica Sinica 29:332–338PubMedGoogle Scholar
  52. Su CC, Wan J, Zhai HQ, Wang CM, Sun LH, Yasui H, Yoshimura A (2005) A new locus for resistance to brown planthopper identified in the indica rice variety DV85. Plant Breed 124:93–95CrossRefGoogle Scholar
  53. Su CC, Zhai HQ, Wang CM, Sun LH, Wan JM (2006) SSR mapping of brown planthopper resistance gene Bph9 in Kaharamana, an indica rice (Oryza sativa L.). Acta Genetica Sinica 33:262–268CrossRefPubMedGoogle Scholar
  54. Sun L, Su C, Wang C, Zhai H, Wan J (2005) Mapping of a major resistance gene to the brown plant hopper in the rice cultivar Rathu Heenati. Breed Sci 55:391–396CrossRefGoogle Scholar
  55. Wang DL, Zhu J, Li ZK, Paterson AH (1999) Mapping QTLs with epistatic effects and QTL × environment interactions by mixed linear model approaches. Theor Appl Genet 99:1255–1264CrossRefGoogle Scholar
  56. Wu JT, Heinrichs EA, Medrano FG (1986) Resistance of wild rice, Oryza spp., to the brown planthopper, Nilaparvata lugens (homoptera: Delphacidae). Environ Entomol 15:648–653Google Scholar
  57. Xu XF, Mei HW, Luo LJ, Cheng XN, Li ZK (2002) RFLP-facilitated investigation of the quantitative resistance of rice to brown planthopper (Nilaparvata lugens). Theor Appl Genet 104:248–253CrossRefPubMedGoogle Scholar
  58. Yang H, Ren X, Weng Q, Zhu L (2002) Molecular mapping and genetic analysis of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene. Hereditas 136:39–43CrossRefPubMedGoogle Scholar
  59. Yang H, You A, Yang Z, Zhang F, He R, Zhu L, He G (2004) High-resolution genetic mapping at the Bph15 locus for brown planthopper resistance in rice (Oryza sativa L.). Theor Appl Genet 110:182–191CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Md Lutfor Rahman
    • 1
  • Wenzhu Jiang
    • 1
  • Sang Ho Chu
    • 1
  • Yongli Qiao
    • 1
  • Tae-Ho Ham
    • 1
  • Mi-Ok Woo
    • 1
  • Joohyun Lee
    • 1
  • M. Sakina Khanam
    • 1
    • 2
  • Joong-Hyoun Chin
    • 3
  • Ji-Ung Jeung
    • 5
  • D. S. Brar
    • 3
  • K. K. Jena
    • 4
  • Hee-Jong Koh
    • 1
    Email author
  1. 1.Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute of Agriculture and Life SciencesSeoul National UniversitySeoulKorea
  2. 2.Department of Crop PhysiologyBangladesh Institute of Nuclear AgricultureMymensinghBangladesh
  3. 3.Plant Breeding, Genetics and Biotechnology DivisionInternational Rice Research InstituteManilaPhilippines
  4. 4.Plant Breeding, Genetics and Biotechnology DivisionInternational Rice Research Institute, C/o IRRI-Korea Office, National Institute of Crop ScienceSuwonKorea
  5. 5.Rice Research DivisionNational Institute of Crop ScienceSuwonKorea

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