Theoretical and Applied Genetics

, Volume 119, Issue 7, pp 1223–1235 | Cite as

Mapping quantitative trait loci for preharvest sprouting resistance in white wheat

  • Jesse D. Munkvold
  • James Tanaka
  • David Benscher
  • Mark E. Sorrells
Original Paper

Abstract

The premature germination of seeds before harvest, known as preharvest sprouting (PHS), is a serious problem in all wheat growing regions of the world. In order to determine genetic control of PHS resistance in white wheat from the relatively uncharacterized North American germplasm, a doubled haploid population consisting of 209 lines from a cross between the PHS resistant variety Cayuga and the PHS susceptible variety Caledonia was used for QTL mapping. A total of 16 environments were used to detect 15 different PHS QTL including a major QTL, QPhs.cnl-2B.1, that was significant in all environments tested and explained from 5 to 31% of the trait variation in a given environment. Three other QTL QPhs.cnl-2D.1, QPhs.cnl-3D.1, and QPhs.cnl-6D.1 were detected in six, four, and ten environments, respectively. The potentially related traits of heading date (HD), plant height (HT), seed dormancy (DOR), and rate of germination (ROG) were also recorded in a limited number of environments. HD was found to be significantly negatively correlated with PHS score in most environments, likely due to a major HD QTL, QHd.cnl-2B.1, found to be tightly linked to the PHS QTL QPhs.cnl-2B.1. Using greenhouse grown material no overlap was found between seed dormancy and the four most consistent PHS QTL, suggesting that greenhouse environments are not representative of field environments. This study provides valuable information for marker-assisted breeding for PHS resistance, future haplotyping studies, and research into seed dormancy.

Notes

Acknowledgments

This research was supported by National Research Initiative Competitive Grants 2005-35301-15728 and 2006-55606-16629 from the USDA Cooperative State Research, Education, and Extension Service and Hatch project, pp 149–419.

Supplementary material

122_2009_1123_MOESM1_ESM.pdf (35 kb)
Supplementary material 1 (PDF 35 kb)
122_2009_1123_MOESM2_ESM.pdf (24 kb)
Supplementary material 2 (PDF 23 kb)
122_2009_1123_MOESM3_ESM.pdf (41 kb)
Supplementary material 3 (PDF 41 kb)
122_2009_1123_MOESM4_ESM.pdf (36 kb)
Supplementary material 4 (PDF 35 kb)

References

  1. Anderson JA, Ogihara Y, Sorrells ME, Tanksley SD (1992) Development of a chromosomal arm map for wheat based on RFLP markers. Theor Appl Genet 83:1035–1043Google Scholar
  2. Anderson JA, Sorrells ME, Tanksley SD (1993) RFLP analysis of genomic regions associated with resistance to preharvest sprouting in wheat. Crop Sci 33:453–459Google Scholar
  3. Bailey PC, Mckibbin RS, Lenton JR, Holdsworth MJ, Flintham JE, Gale MD (1999) Genetic map locations for orthologous Vp1 genes in wheat and rice. Theor Appl Genet 98:281–284CrossRefGoogle Scholar
  4. Basten CJ, Weir BS, Zeng ZB (1994) Zmap—a QTL Cartographer. In: Smith C, Gavora JS, Benkel B, Chesnais J, Fairfull W, Gibson JP et al (eds) Proceedings of the 5th World congress on genetics applied to livestock production: Computing Strategies and Software, Guelph, Ontario, CanadaGoogle Scholar
  5. Basten CJ, Weir BS, Zeng ZB (2002) QTL Cartographer. Department of Statistics, North Carolina State University, Raleigh, NCGoogle Scholar
  6. Beales J, Turner A, Griffiths S, Snape JW, Laurie DA (2007) A pseudo-response regulator is misexpressed in the photoperiod insensitive Ppd-D1a mutant of wheat (Triticum aestivum L.). Theor Appl Genet 115:721–733CrossRefPubMedGoogle Scholar
  7. Bewley JD, Black M (1994) Seeds:Physiology of Development and Germination. Plenum Press, New YorkGoogle Scholar
  8. Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971PubMedGoogle Scholar
  9. Ellis H, Spielmeyer W, Gale R, Rebetzke J, Richards A (2002) “Perfect” markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat. Theor Appl Genet 105:1038–1042CrossRefPubMedGoogle Scholar
  10. Flintham J (2000) Different genetic components control coat-imposed and embryo-imposed dormancy in wheat. Seed Sci Res 10:43–50Google Scholar
  11. Flintham J, Adlam R, Bassoi M, Holdsworth M, Gale M (2002) Mapping genes for resistance to sprouting damage in wheat. Euphytica 126:39–45CrossRefGoogle Scholar
  12. Groos C, Gay G, Perretant M, Gervais L, Bernard M, Dedryver F et al (2002) Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a white × red grain bread-wheat cross. Theor Appl Genet 104:39–47CrossRefPubMedGoogle Scholar
  13. Gupta PK, Balyan HS, Edwards KJ, Isaac P, Korzun V, Röder M et al (2002) Genetic mapping of 66 new microstalleite (SSR) loci in bread wheat. Theor Appl Genet 105:413–422CrossRefPubMedGoogle Scholar
  14. Guyomarc’h H, Charmet G, Edwards KJ, Bernard M (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat. Theor Appl Genet 104:1164–1172CrossRefPubMedGoogle Scholar
  15. Hanocq E, Niarquin M, Heumez E, Rousset M, Le Gouis J (2004) Detection and mapping of QTL for earliness components in a bread wheat recombinant inbred lines population. Theor Appl Genet 110:106–115CrossRefPubMedGoogle Scholar
  16. Heun M, Kennedy AE, Anderson JA, Lapitan NLV, Sorrells ME, Tanksley SD (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare). Genome 34:437–447Google Scholar
  17. Imtiaz M, Ogbonnaya FC, Oman J, Van Ginkel M (2008) Characterization of quantitative trait loci controlling genetic variation for preharvest sprouting in synthetic backcross-derived wheat lines. Genetics 178:1725–1736CrossRefPubMedGoogle Scholar
  18. Kato K, Nakamura W, Tabiki T, Miura H (2001) Detection of loci controlling seed dormancy on group 4 chromosomes of wheat and comparative mapping with rice and barley genomes. Theor Appl Genet 10:980–985CrossRefGoogle Scholar
  19. King RW, Richards RA (1984) Water uptake in relation to pre-harvest sprouting damage in wheat:Ear characteristics. Aust J Agric Res 35:327–336CrossRefGoogle Scholar
  20. King RW, Von Wettstein-Knowles P (2000) Epicuticular waxes and regulation of ear wetting and pre-harvest sprouting in barley and wheat. Euphytica 112:157–166CrossRefGoogle Scholar
  21. Kulwal PL, Singh R, Balyan HS, Gupta PK (2004) Genetic basis of pre-harvest sprouting tolerance using single-locus and two-locus QTL analyses in bread wheat. Funct Integr Genomics 4:94–101CrossRefPubMedGoogle Scholar
  22. Kulwal PL, Kumar N, Gaur A, Khurana P, Khurana JP, Tyagi AK et al (2005) Mapping of a major QTL for pre-harvest sprouting tolerance on chromosome 3A in bread wheat. Theor Appl Genet 111:1052–1059CrossRefPubMedGoogle Scholar
  23. Liu ZH, Anderson JA, Hu J, Friesen TL, Rasmussen JB, Faris JD (2005) A wheat intervarietal genetic linkage map based on microsatellite and target region amplified polymorphism markers and its utility for detecting quantitative trait loci. Theor Appl Genet 111:782–794CrossRefPubMedGoogle Scholar
  24. Liu S, Cai S, Graybosch R, Chen C, Bai G (2008) Quantitative trait loci for resistance to pre-harvest sprouting in US hard white winter wheat Rio Blanco. Theor Appl Genet 117:691–699CrossRefPubMedGoogle Scholar
  25. Mangin B, Goffinet B, Rebai A (1994) Constructing confidence intervals for QTL location. Genetics 138:1301–1308PubMedGoogle Scholar
  26. Manly KF, Cudmore RH, Meer M (2001) Map manager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932CrossRefPubMedGoogle Scholar
  27. Mares DJ, Mrva K (2001) Mapping quantitative trait loci associated with variation in grain dormancy in Australian wheat. Aust J Agric Res 52:1257–1265CrossRefGoogle Scholar
  28. Mares DJ, Mrva K, Tan MK, Sharp P (2002) Dormancy in white-grained wheat:Progress towards identification of genes and molecular markers. Euphytica 126:47–53CrossRefGoogle Scholar
  29. Mares D, Mrva K, Cheong J, Williams K, Watson B, Storlie E et al (2005) A QTL located on chromosome 4A associated with dormancy in white- and red-grained wheats of diverse origin. Theor Appl Genet 111:1357–1364CrossRefPubMedGoogle Scholar
  30. Miura H, Sato N, Kato K, Amano Y (2002) Detection of chromosomes carrying genes for seed dormancy of wheat using the backcross reciprocal monosomic method. Plant Breeding 121:394–399CrossRefGoogle Scholar
  31. Mori M, Uchino N, Chono M, Kato K, Miura H (2005) Mapping QTLs for grain dormancy on wheat chromosome 3A and the group 4 chromosomes, and their combined effect. Theor Appl Genet 110:1257–1266CrossRefGoogle Scholar
  32. Mrva K, Mares DJ (1996) Expression of late maturity alpha-amylase in wheat containing gibberellic acid insensitivity genes. Euphytica 88:69–76CrossRefGoogle Scholar
  33. Ogbonnaya FC, Imtiaz M, Depauw RM (2007) Haplotype diversity of preharvest sprouting QTLs in wheat. Genome 50:107–118CrossRefPubMedGoogle Scholar
  34. Ogbonnaya FC, Imtiaz M, Ye G, Hearnden PR, Hernandez E, Eastwood RF et al (2008) Genetic and QTL analyses of seed dormancy and preharvest sprouting resistance in the wheat germplasm CN10955. Theor Appl Genet 116:891–902CrossRefPubMedGoogle Scholar
  35. Osa M, Kato K, Mori M, Shindo C, Torada A, Miura H (2003) Mapping QTLs for seed dormancy and the Vp1 homologue on chromosome 3A in wheat. Theor Appl Genet 106:1491–1496PubMedGoogle Scholar
  36. Pestsova E, Ganal MW, Röder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43:689–697CrossRefPubMedGoogle Scholar
  37. Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M, Leroy P et al (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  38. Roy J, Prasad M, Varshney R, Balyan H, Blake T, Dhaliwal HS et al (1999) Identification of a microsatellite on chromosomes 6B and a STS on 7D of bread wheat showing an association with preharvest sprouting tolerance. Theor Appl Genet 99:336–340CrossRefGoogle Scholar
  39. Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365–386PubMedGoogle Scholar
  40. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114CrossRefPubMedGoogle Scholar
  41. Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J et al (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550–560CrossRefPubMedGoogle Scholar
  42. Sourdille P, Gandon B, Chiquet V, Nicot N, Somers D, Murigneux A et al (2004) Wheat Genoplante SSR mapping data release:A new set of markers and comprehensive genetic and physical mapping data. Graingenes. http://wheat.pw.usda.gov
  43. Tan MK, Sharp PJ, Lu MQ, Howes N (2006) Genetics of grain dormancy in a white wheat. Aust J Agr Res 57:1157–1165CrossRefGoogle Scholar
  44. Torada A, Amano Y (2002) Effect of seed coat color on seed dormancy in different environments. Euphytica 126:99–105CrossRefGoogle Scholar
  45. Torada A, Koike M, Ikeguchi S, Tsutsui I (2008) Mapping of a major locus controlling seed dormancy using backcrossed progenies in wheat (Triticum aestivum L.). Genome 51:426–432CrossRefPubMedGoogle Scholar
  46. Vos P, Hogers R, Bleeker M, Reijans M, Van De Lee T et al (1995) AFLP:A new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414CrossRefPubMedGoogle Scholar
  47. Walker-Simmons M (1987) ABA levels and sensitivity in developing wheat embryos of sprouting resistant and susceptible cultivars. Plant Physiol 84:61–66CrossRefPubMedGoogle Scholar
  48. Walker-Simmons M (1988) Enhancement of ABA responsiveness in wheat embryos by high temperature. Plant Cell Environ 11:769–775CrossRefGoogle Scholar
  49. Wu J, Carver BF, Goad CL (1999) Kernel color variability of hard white and hard red winter wheat. Crop Sci 39:634–638CrossRefGoogle Scholar
  50. Yang J, Zhu J, Williams RW (2007a) Mapping the genetic architecture of complex traits in experimental populations. Bioinformatics 23:1527–1536CrossRefPubMedGoogle Scholar
  51. Yang Y, Zhao XL, Xia LQ, Chen XM, Xia XC, Yu Z et al (2007b) Development and validation of a Viviparous-1 STS marker for pre-harvest sprouting tolerance in Chinese wheats. Theor Appl Genet 115:971–980CrossRefPubMedGoogle Scholar
  52. Yang J, Hu C, Hu H, Yu R, Xia Z, Ye X et al (2008) QTLNetwork:Mapping and visualizing genetic architecture of complex traits in experimental populations. Bioinformatics 24:721–723CrossRefPubMedGoogle Scholar
  53. Yu J, Dake TM, Singh S, Benscher D, Li W, Gill B et al (2004) Development and mapping of EST-derived simple sequence repeat markers for hexaploid wheat. Genome 47:805–818CrossRefPubMedGoogle Scholar
  54. Zanetti S, Winzeler M, Keller M, Keller B, Messmer M (2000) Genetic analysis of pre-harvest sprouting resistance in a wheat × spelt cross. Crop Sci 40:1406–1417Google Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Jesse D. Munkvold
    • 1
    • 2
  • James Tanaka
    • 1
  • David Benscher
    • 1
  • Mark E. Sorrells
    • 1
    • 3
  1. 1.Department of Plant Breeding and GeneticsCornell UniversityIthacaUSA
  2. 2.Boyce Thompson Institute for Plant ResearchIthacaUSA
  3. 3.Department of Plant Breeding and GeneticsCornell UniversityIthacaUSA

Personalised recommendations