Theoretical and Applied Genetics

, Volume 119, Issue 5, pp 795–803 | Cite as

Inheritance and molecular mapping of a downy mildew resistance gene, Pl 13 in cultivated sunflower (Helianthus annuus L.)

  • Sujatha Mulpuri
  • Zhao Liu
  • Jiuhuan Feng
  • Thomas J. Gulya
  • Chao-Chien Jan
Original Paper

Abstract

The inheritance of resistance to sunflower downy mildew (SDM) derived from HA-R5 conferring resistance to nine races of the pathogen has been determined and the new source has been designated as Pl 13 . The F2 individuals and F3 families of the cross HA-R5 (resistant) × HA 821 (susceptible) were screened against the four predominant SDM races 300, 700, 730, and 770 in separate tests which indicated dominant control by a single locus or a cluster of tightly linked genes. Bulked segregant analysis (BSA) was carried out on 116 F2 individuals with 500 SSR primer pairs that resulted in the identification of 10 SSR markers of linkage groups 1 (9 markers) and 10 (1 marker) of the genetic map (Tang et al. in Theor Appl Genet 105:1124–1136, 2002) that distinguished the bulks. Of these, the SSR marker ORS 1008 of linkage group 10 was tightly linked (0.9 cM) to the Pl 13 gene. Genotyping the F2 population and linkage analysis with 20 polymorphic primer pairs located on linkage group 10 failed to show linkage of the markers with downy mildew resistance and the ORS 1008 marker. Nevertheless, validation of polymorphic SSR markers of linkage group 1 along with six RFLP-based STS markers of linkage group 12 of the RFLP map of Jan et al. (Theor Appl Genet 96:15–22, 1998) corresponding to linkage group 1 of the SSR map, mapped seven SSR markers (ORS 965-1, ORS 965-2, ORS 959, ORS 371, ORS 716, and ORS 605) including ORS 1008 and one STS marker (STS10D6) to linkage group 1 covering a genetic distance of 65.0 cM. The Pl 13 gene, as a different source with its location on linkage group 1, was flanked by ORS 1008 on one side at a distance of 0.9 cM and ORS 965-1 on another side at a distance of 5.8 cM. These closely linked markers to the Pl 13 gene provide a valuable basis for marker-assisted selection in sunflower breeding programs.

Notes

Acknowledgments

We thank Lisa Brown for assistance in downy mildew phenotyping and Angelia Hogness for help with gel analysis and for valuable suggestions. We also thank Larry G. Campbell, Gerald J. Seiler, and Brady A. Vick for critical review of the manuscript. The financial support received by Sujatha Mulpuri from the Department of Biotechnology, GOI, India, is duly acknowledged.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Sujatha Mulpuri
    • 1
  • Zhao Liu
    • 2
  • Jiuhuan Feng
    • 2
  • Thomas J. Gulya
    • 3
  • Chao-Chien Jan
    • 3
  1. 1.Directorate of Oilseeds ResearchHyderabadIndia
  2. 2.Department of Plant SciencesNorth Dakota State UniversityFargoUSA
  3. 3.Northern Crop Science Laboratory, Agricultural Research ServiceU.S. Department of AgricultureFargoUSA

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