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SSR and SCAR mapping of a multiple-allele male-sterile gene in Chinese cabbage (Brassica rapa L.)

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Abstract

The genic multiple-allele inherited male-sterile gene Ms in Chinese cabbage (Brassica rapa L.) was identified as a spontaneous mutation. Applying this gene to hybrid seed production, several B. rapa cultivars have been successfully bred in China. A BC1 population (244 plants) was constructed for mapping the Ms gene. Screening 268 simple sequence repeat (SSR) markers which cover the entire genome of Chinese cabbage was performed with bulked segregant analysis (BSA). On the basis of linkage analysis, the Ms gene was located on linkage group R07. In addition, through the amplified fragment length polymorphism (AFLP) and the sequence-characterized amplified region (SCAR) techniques combining BSA, two SCAR markers which were converted from corresponding AFLP markers flanked the Ms gene. Finally, a genetic map of the Ms gene was constructed covering a total interval of 9.0 cM. Two SCAR markers, syau_scr01 and syau_scr04, flanked the Ms gene at distances of 0.8 and 2.5 cM, respectively. All the SSR markers (cnu_m273, cnu_m030, cnu_m295, and syau_m13) were mapped on the same side of the gene as syau_scr04, the nearest one of which, syau_m13, was mapped at a distance of 3.3 cM. These SSR and SCAR markers may be useful in marker-assisted selection and map-based cloning.

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Acknowledgments

This research was supported by grants from the National Natural Science Foundation of China (No. 30671414), the National High Technology Research and Development Program of China (863 Program) (No. 2006AA10Z170), and the Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea (No. 607003-05).

Author information

Correspondence to Hui Feng.

Additional information

Communicated by C. Quiros.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (TIFF 603 kb) S1 Examples of two SSR markers linked to the Ms gene screened in BC1 plants. Polyacrylamide gel electrophoresis of PCR products revealed the SSR markers cnu_m273 (A) and syau_m13 (B) in BC1 plants. Ps: male-sterile parent; Pf: fertile parent; Bf1–Bf15: fertile plants (msms); Bs1–Bs15: genic male-sterile plants (Msms). Recombinants are shown by asterisks

Supplementary material 2 (TIFF 588 kb)

Supplementary material 3 (TIFF 598 kb) S2 Examples of SCAR markers screened in BC1 plants. (A) Agarose gel electrophoresis of PCR products revealed the SCAR marker syau_scr01 in BC1 plants. (B) Polyacrylimade gel electrophoresis of PCR products revealed the SCAR marker syau_scr04 in BC1 plants. Ps: male-sterile parent; Pf: fertile parent; Bf1–Bf15: fertile plants (msms); Bs1–Bs15: genic male-sterile plants (Msms). Recombinants are shown by asterisks

Supplementary material 4 (TIFF 357 kb)

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Feng, H., Wei, P., Piao, Z. et al. SSR and SCAR mapping of a multiple-allele male-sterile gene in Chinese cabbage (Brassica rapa L.). Theor Appl Genet 119, 333–339 (2009). https://doi.org/10.1007/s00122-009-1042-1

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Keywords

  • Amplify Fragment Length Polymorphism
  • Simple Sequence Repeat Marker
  • Chinese Cabbage
  • Amplify Fragment Length Polymorphism Marker
  • Bulk Segregant Analysis