Theoretical and Applied Genetics

, Volume 119, Issue 2, pp 271–280 | Cite as

Genetics and mapping of adult plant rust resistance in soybean PI 587886 and PI 587880A

  • Jeffery D. Ray
  • Wilfrido Morel
  • James R. Smith
  • Reid D. Frederick
  • Monte R. Miles
Original Paper

Abstract

Two soybean accessions, PI 587886 and PI 587880A, previously identified as having resistance to Phakospora pachyrhizi Syd. (soybean rust, SBR) were used to create two populations (POP-1 and POP-2) segregating for SBR resistance. F2-derived F3 (F2:3) families from each population were grown in a naturally SBR-infected field in Paraguay to determine inheritance and map resistance genes. Over 6,000 plants from 178 families in POP-1 and over 5,000 plants from 160 families in POP-2 were evaluated at R5 for lesion type: immune reaction (IR), reddish-brown (RB), or tan (TAN) colored lesions. Based on the lesion type present, each F2:3 family was rated as resistant, segregating or susceptible and this classification was used to infer the F2-phenotype and genotype. For both populations, the F2 segregation ratios fit a 1:2:1 (resistant:segregating:susceptible) ratio expected for a single gene (P > 0.05). The RB lesions occurred almost exclusively in the heterozygous class, indicating incomplete dominance under the conditions of this study. Molecular markers flanking the locations of the known resistance genes were used to map the resistance gene in both populations to the Rpp1 locus. However, evaluation of PI 587886 and PI 587880A against eight P. pachyrhizi isolates indicated that the resistance allele in these two accessions was different from Rpp1. This test also demonstrated that these accessions were resistant to at least one P. pachyrhizi isolate collected in the southern US. This is the first report of using an adult plant field-screen with natural rust pressure to map SBR resistance.

Notes

Acknowledgments

The authors appreciate the dedicated work of the USDA-ARS personnel at Stoneville, MS, including Ms. Angelie Davis, Mr. Hans Hinrichsen, Mr. Philip Handly, Mr. Ronnie Griffin, Mr. Jeffrey Britt and Ms. Loria Courtney. In Paraguay we were ably assisted in the field and laboratory by Ms. Noelia Bogado, Mr. Ismael Morel, Mr. Francisco Saucedo, Mr. Claudio Vigo, Ms. Diana Bado, Ms. Sergia Portillo, and Ms. Nidia Benitez. The Paraguay based portion of this work was critically facilitated by Mr. Luis Enrique Robledo of DIA and Mr. Luis E. Cubilla of CAPECO. Seed production in Puerto Rico by Ricardo Goenaga and Carlos Torres of the USDA-ARS Tropical Agriculture Research Station was critical for the timely completion of this project. We also appreciate the efforts of Ms. Christine Stone for assistance with the inoculations at the FDWSRU. This research could not have been accomplished without these combined efforts.

References

  1. Arelli P, Young LD, Mengistu A (2006) Registration of high yielding and multiple disease resistant germplasm JTN5503. Crop Sci 46:2723–2724CrossRefGoogle Scholar
  2. Bernard RL (1967) The inheritance of pod color in soybeans. J Hered 58:165–168CrossRefGoogle Scholar
  3. Bernard RL, Cremeens CR (1988) Registration of ‘Williams 82’ soybean. Crop Sci 28:1027–1028Google Scholar
  4. Bonde MR, Nester SE, Austin CN, Stone CL, Frederick RD, Hartman GL, Miles MR (2006) Evaluation of virulence of Phakopsora pachyrhizi and P. meibomiae isolates. Plant Dis 90:708–716CrossRefGoogle Scholar
  5. Bromfield KR (1984) Soybean rust. Monograph No. 11, The American Phytopathological Society, St. PaulGoogle Scholar
  6. Calvo ES, Kiihl RAS, Garcia A, Harada A, Hiromoto DM (2008) Two major recessive soybean genes conferring soybean rust resistance. Crop Sci 48:1350–1354CrossRefGoogle Scholar
  7. Chakraborty N, Curley J, Frederick RD, Hyten DL, Nelson RL, Hartman GL, Diers BW (2009) Mapping and confirmation of a new allele at Rpp1 from soybean PI 594538A conferring RB lesion type resistance to soybean rust. Crop Sci (accepted)Google Scholar
  8. Cherry E, Peet C (1966) An efficient device for the rapid collection of fungal spores from infected plants. Phytopatholgy 56:1102–1103Google Scholar
  9. Fehr WR, Caviness CE (1977) Stages of soybean development. Iowa Agric Exp Stn Spec Rep 80. Iowa State University, Ames, IAGoogle Scholar
  10. Garcia A, Calvo ES, Kiihl RS, Harada A, Hiromoto DM, Vieira LGE (2008) Molecular mapping of soybean rust (Phakopsora pachyrhizi) resistance genes: discovery of a novel locus and alleles. Theor Appl Genet 117:545–553PubMedCrossRefGoogle Scholar
  11. Hartwig EE (1986) Identification of a fourth major gene conferring resistance to soybean rust. Crop Sci 26:1135–1136Google Scholar
  12. Hartwig EE, Bromfield KR (1983) Relationships among three genes conferring specific resistance to rust in soybeans. Crop Sci 23:237–239Google Scholar
  13. Hyten DL, Hartman GL, Nelson RL, Frederick RD, Concibido VC, Narvel JM, Cregan PB (2007) Map location of the Rpp1 locus that confers resistance to soybean rust in soybean. Crop Sci 47:837–840CrossRefGoogle Scholar
  14. Hyten DL, Smith JR, Frederick RD, Tuker ML, Song Q, Cregan PB (2009) Bulk segregate analysis using the GoldenGate assay to locate the Rpp3 locus that confers resistance to Phakopsora pachyrhizi (soybean rust) in soybean. Crop Sci 49:265–271CrossRefGoogle Scholar
  15. McLean R, Byth DE (1980) Inheritance of resistance to rust (Phakopsora pachyrhizi) in soybean. Aust J Agric Res 31:951–956CrossRefGoogle Scholar
  16. Melching JS, Bromfield KR, Kingsolver CH (1983) The plant pathogen containment facility at Frederick, Maryland. Plant Dis 67:717–722CrossRefGoogle Scholar
  17. Miles MR, Frederick RD, Hartman GL (2006) Evaluation of soybean germplasm for resistance to Phakopsora pachyrhizi. Plant Health Prog. doi:10.1094.PHP-2006=0104-01-RS
  18. Miles MR, Morel W, Ray JD, Smith JR, Frederick RD, Hartman GL (2008) Adult plant evaluation of soybean accessions for resistance to Phakopsora pachyrhizi in the field and greenhouse in Paraguay. Plant Dis 92:96–105CrossRefGoogle Scholar
  19. Monteros MJ, Missaoui AM, Phillips DV, Walker DR, Boerma HR (2007) Mapping and confirmation of the “Hyuuga” RB lesion resistance gene for Asian soybean rust. Crop Sci 47:829–834Google Scholar
  20. Morel W, Scheid N, Amarilla V, Cubilla LE (2004) Soybean rust in Paraguay, evolution in the past three years. In: Moscardi J, Hoffman-Campo CB, Ferreira Saraiva O, Galerani PR, Krzyzanowski FC, Carrão-Panizzi MC (eds) Proceedings of VII World soybean research conference, IV international soybean processing and utilization conference, III Congresso Mundial de Soja (Brazilian soybean conference). Emprapa Soja, Londrina, Brazil, pp 361–364Google Scholar
  21. Palmer RG, Pfeiffer TW, Buss GR, Kilen TC (2004) Qualitative genetics. In: Boerma HR, Specht JE (eds) Soybeans: improvement, production, and uses, 3rd edn, Agronomy Monography 16. American Society of Agronomy, Madison, pp 137–233Google Scholar
  22. Pham TA, Miles MR, Frederick RD, Hill CB, Hartman GL (2009) Differential responses of resistant soybean entries to isolates of Phakopsora pachyrhizi. Plant Dis 93:224–228CrossRefGoogle Scholar
  23. Schneider RW, Hollier CA, Whitam HK, Palm ME, McKenny JM, Hernandez JR, Levy L, Devries-Paterson R (2005) First report of soybean rust caused by Phakopsora pachyrhizi in the continental United States. Plant Dis 89:774CrossRefGoogle Scholar
  24. Silva DCG, Yamanaka N, Brogin RL, Arias CAA, Nepomuceno AL, Di Mauro AO, Pereira SS, Nogueira LM, Passianotto ALL, Abdelnoor RV (2008) Molecular mapping of two loci that confer resistance to Asian rust in soybean. Theor Appl Genet 117:57–63PubMedCrossRefGoogle Scholar
  25. Sing BB, Thapliyal PN (1977) Breeding for resistance to soybean rust in India. p. 63-65. In: Ford RE, Sinclair JB (eds) Rust of soybean-the problem and research needs. Report of a workshop held in Manila, the Philippines, 28 February–4 March 1977. Inter Agri Pub, INTSOY Series Number 12Google Scholar
  26. Sokal RR, Rohlf FJ (1995) Biometry, 3rd edn. (W.H.) Freeman, New York, p 887Google Scholar
  27. Song QJ, Marek LF, Shoemaker RC, Lark KG, Concibido VC, Delannay X, Specht JE, Cregan PB (2004) A new integrated genetic linkage map of the soybean. Theor Appl Genet 109:122–128PubMedCrossRefGoogle Scholar
  28. Van Ooijen JW (2006) JoinMap 4®, Software for the calculation of genetic linkage maps in experimental populations. Kyazma B.V., Wageningen, The NetherlandsGoogle Scholar
  29. Yates F (1934) Contingency table involving small numbers and the χ2 test. J R Stat Soc (Suppl) 1:217–235CrossRefGoogle Scholar
  30. Yorinori JT, Paiva WM, Frederick RD, Costamilan LM, Bertagnolli PF, Hartman GL, Godoy CV, Nunes J Jr (2005) Epidemics of soybean rust (Phakopsora pachyrhizi) in Brazil and Paraguay from 2001 to 2003. Plant Dis 89:675–677CrossRefGoogle Scholar

Copyright information

© US Government 2009

Authors and Affiliations

  • Jeffery D. Ray
    • 1
  • Wilfrido Morel
    • 2
  • James R. Smith
    • 1
  • Reid D. Frederick
    • 3
  • Monte R. Miles
    • 4
  1. 1.USDA-ARS, Crop Genetics and Production Research UnitStonevilleUSA
  2. 2.Centro Regional de Investigación Agrícola, Capitán MirandaItapúaParaguay
  3. 3.USDA-ARS, Foreign Disease-Weed Science Research UnitFt. DetrickUSA
  4. 4.USDA-ARS, National Soybean Research CenterUniversity of IllinoisUrbanaUSA

Personalised recommendations